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Immunofluorescence tomography detection card for detecting antigen of CPV (canine parvovirus) and preparation method

A canine parvovirus and immunofluorescence technology, applied in the field of immunofluorescence chromatography detection cards, can solve the problems of inability to fully meet the detection requirements, poor repeatability and stability, and poor specificity, and achieve fast detection speed, sufficient release, The effect of reducing inspection costs

Pending Publication Date: 2018-08-17
广州敏捷生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses a self-made polyclonal antibody against canine parvovirus antigen as a coating antibody, which may have cross-reaction with other similar viruses, and the specificity is not good.
In addition, the patent adopts the traditional colloidal gold chromatography technology, and the immune marker relies on electrostatic adsorption, which has defects such as low sensitivity, difficulty in quantification, poor repeatability and stability, and cannot fully meet the clinical detection needs.

Method used

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  • Immunofluorescence tomography detection card for detecting antigen of CPV (canine parvovirus) and preparation method
  • Immunofluorescence tomography detection card for detecting antigen of CPV (canine parvovirus) and preparation method
  • Immunofluorescence tomography detection card for detecting antigen of CPV (canine parvovirus) and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0058] An immunofluorescence chromatography detection card for detecting canine parvovirus antigen provided by the present invention, see Figure 1 to Figure 2 , including a housing 7, the housing 7 is provided with an immunofluorescence chromatography detection card for detecting canine parvovirus antigen, and the immunofluorescence chromatography detection card for detecting canine parvovirus antigen includes a bottom plate 1, A sample pad 2, a binding pad 3, a nitrocellulose membrane 4, and a water-absorbing pad 5 are sequentially connected on the base plate 1, and the binding pad 3 is provided with a fluorescent microsphere-labeled CPV monoclonal antibody 31 and a fluorescent microsphere-labeled goat anti-chicken antibody. IgY32, the nitrocellulose membrane 4 is provided with a quality control C line coated with chicken IgY, and a detection T line coated with CPV monoclonal antibody. The housing 7 is provided with a sample injection hole 72 compatible with the sample pad 2...

Embodiment 2

[0060] Another immunofluorescence chromatography test card for detecting canine parvovirus antigen provided by this application, see Figure 1 to Figure 3 , including a box body 9, a sample diluent bottle 6, a suction pipe 8 and a detection card are arranged in the described body 9, and the detection card includes a housing 7, and the housing 7 is provided with an antigen for detecting canine parvovirus antigen. The immunofluorescence chromatography detection card of the above-mentioned method comprises an immunofluorescence chromatography detection card base plate 1 for detecting canine parvovirus antigen, and a sample pad 2, a binding pad 3, a nitrocellulose membrane 4 and a water-absorbent pad are sequentially connected on the base plate 1 5. The binding pad 3 is provided with fluorescent microsphere-labeled CPV monoclonal antibody 31 and fluorescent microsphere-labeled goat anti-chicken IgY32, and the nitrocellulose membrane 4 is provided with a quality control line C coate...

Embodiment 3

[0062] The preparation method of the immunofluorescence chromatography test card for detecting canine parvovirus antigen provided in Example 1 and Example 2 is assembled at a temperature of (18-26) ° C and an environment with a humidity of ≤ 30%, on the bottom plate 1 The nitrocellulose membrane 4, the water-absorbing pad 5, the binding pad 3 and the sample pad 2 are successively connected; the pasted large plate is cut into 4.0mm wide test strips, and put into a plastic card case, that is, the CPV test card; The test card is placed in an aluminum film bag, add 1 pack of desiccant, and heat seal it for later use.

[0063] The specific preparation method is as follows:

[0064] 1.1 Preparation of conjugation pads

[0065] 1.1.1 Detection of T-line antibody labeling

[0066] 1) Select microspheres: microspheres are polystyrene microspheres containing fluorescent mismatches of rare earth europium elements; density: 1.05g / cm3; refractive index: 1.59@589nm, 25°C; diameter 300nm; ...

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Abstract

The invention discloses an immunofluorescence tomography detection card for detecting antigen of CPV (canine parvovirus) and a preparation method, aims at providing the immunofluorescence tomography detection card for detecting the antigen of the CPV with the advantages of high density and reliable detection results, and belongs to the field of detection of animal epidemic diseases. The immunofluorescence tomography detection card is technically characterized by comprising a bottom board, wherein the bottom board is sequentially connected with a sample pad, a bonding pad, a nitrocellulose membrane and a water absorbing pad; a fluorescent microsphere-marked CPV monoclonal antibody and fluorescent microsphere-marked goat anti-chicken IgY are arranged on the bonding pad; a chicken IgY-coatedquality control line C, and a CPV monoclonal antibody-coated detection line T are arranged on the nitrocellulose membrane.

Description

technical field [0001] The invention discloses an immunofluorescence chromatography detection card, specifically, an immunofluorescence chromatography detection card for detecting canine parvovirus antigen, and the invention also discloses a preparation method of the immunofluorescence chromatography detection card . Background technique [0002] Canine parvovirus (CPV) mainly infects dogs, especially puppies. It is highly contagious and has a high mortality rate. All can fall ill all the year round, with winter, spring frequently. Sudden changes in feeding and management conditions, long-distance transportation, cold, and crowding can all contribute to the occurrence of this disease. Sick dogs are the main source of infection, and there are a lot of viruses in vomit, saliva, and feces. Rehabilitation dogs can still expel toxins through feces for a long time. There is evidence that humans, lice, flies, and cockroaches can be mechanical carriers of CPV. Healthy dogs are ...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/577G01N33/533
CPCG01N33/533G01N33/56983G01N33/577
Inventor 汤永平梁展鹏
Owner 广州敏捷生物技术有限公司
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