Centrifugal force micro fluidic chip used for nucleic acid extraction

A microfluidic chip, centrifugal force technology, applied in specific-purpose bioreactors/fermenters, sterilization methods, enzymology/microbiology devices, etc., can solve the problem of reduced efficiency of extraction steps, high equipment requirements, and sample loss. and other problems, to achieve the effect of saving resources, low energy consumption, and small sample volume

Inactive Publication Date: 2018-08-21
NANJING UNIV +1
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Problems solved by technology

At present, laboratories often use nucleic acid extraction kits to extract nucleic acids. Most of them use the spin column method, which requires multiple centrifuges and requires relatively high external equipment. At the same time, the required sample volume is relatively large, and it is easy to cause sample loss; time-consuming and tedious extraction step greatly reduces the efficiency of the experiment

Method used

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  • Centrifugal force micro fluidic chip used for nucleic acid extraction
  • Centrifugal force micro fluidic chip used for nucleic acid extraction
  • Centrifugal force micro fluidic chip used for nucleic acid extraction

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Embodiment Construction

[0038] A centrifugal force microfluidic chip, which uses PDMS integrated with a wirelessly controlled paraffin valve and other microvalves (preferably capillary valves) and a chip with corresponding circuits to drive the centrifugal platform of the entire chip, and control the following through the wirelessly controlled paraffin valve One or a combination of the contents: introduction of samples, mixing of samples, control of nucleic acid collection and waste collection through a combination of wirelessly controlled paraffin valves and other microvalves (preferably capillary valves). And the unique silica gel membrane structure is used to realize the integrated process from sample to result.

[0039] The centrifugal force microfluidic chip has a four-layer structure, including four parts: a liquid reaction layer, a controlled circuit layer including a heating resistor, a main control circuit layer, and a power supply layer; the controlled circuit layer is below the liquid react...

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Abstract

The invention provides a centrifugal force micro fluidic chip used for nucleic acid extraction, and a nucleic acid extraction method using the centrifugal force micro fluidic chip. The centrifugal force micro fluidic chip is composed of PDMS formed via integration of wirelessly controlled paraffin valves and other micro valves (preferable capillary micro valves) and corresponding circuits, and iscapable of realizing sample extraction integrated process including sample introduction, impurity washing, nucleic acid adsorption, and elution. Efficiency is high, speed is fast, and resource is saved.

Description

technical field [0001] The invention relates to the field of nucleic acid extraction, in particular to a centrifugal force microfluidic chip for nucleic acid extraction. Background technique [0002] The separation and purification technology of biological macromolecular nucleic acid is the basis of molecular biology research and the key technology of life science research and application. The efficiency and quality of nucleic acid acquisition directly affect the results of subsequent experiments. At present, laboratories often use nucleic acid extraction kits to extract nucleic acids. Most of them use the spin column method, which requires multiple centrifuges and requires relatively high external equipment. At the same time, the required sample volume is relatively large, and it is easy to cause sample loss; time-consuming and tedious extraction Steps greatly reduce the efficiency of experiments. Therefore, it has become an urgent need to develop a nucleic acid extractio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/12C12M1/00C12N15/10
CPCC12N15/1017
Inventor 王光辉王夷惠李忠文谭杰其他发明人请求不公开姓名
Owner NANJING UNIV
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