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Preparation method of electrochemical working electrode and detection method of blood sugar

A working electrode and electrochemical technology, which is applied in the field of electrochemical detection of blood sugar, can solve the problems of unstable attachment and inapplicability of nano-silver wires, and achieve the effects of uniform size, convenient detection of blood sugar, and low concentration of hydrogen peroxide

Active Publication Date: 2020-07-03
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also various working electrodes used in the electrochemical detection method of blood sugar, such as ITO / Ag working electrodes, but these methods have limitations, and the silver nanowires cannot be stably attached to the ITO glass sheet, so this method is not applicable

Method used

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  • Preparation method of electrochemical working electrode and detection method of blood sugar
  • Preparation method of electrochemical working electrode and detection method of blood sugar
  • Preparation method of electrochemical working electrode and detection method of blood sugar

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Preparation of conductive nanomaterials:

[0034] Measure 50mL of ethylene glycol solution into a three-necked flask, heat at 165°C for 1.2h, and weigh 1.4mg of CuCl 2 , 1.2740g AgNO 3 , respectively dissolved in 2.6mL and 15mL of ethylene glycol solution, where CuCl 2 At a concentration of 0.004M, AgNO 3 The concentration of polyvinylpyrrolidone is 0.05M, and then 3.7296g of polyvinylpyrrolidone with a molecular weight of 29000 is weighed and dissolved in 48mL of ethylene glycol, wherein the concentration of the ethylene glycol solution of polyvinylpyrrolidone is 0.7M (in terms of polyvinylpyrrolidone monomer), 2.6mL of CuCl 2 solution and 15 mL of AgNO 3 Pour the solution into a three-necked bottle, and finally add 48mL of polyvinylpyrrolidone solution dropwise to the reaction solution at a rate of 48mL / h. After the titration, stop heating, cool to room temperature, settle with acetone, and then wash with acetone. Centrifuge and finally disperse the product in et...

Embodiment 2

[0039] Preparation of conductive nanomaterials:

[0040] Measure 50mL of ethylene glycol solution into a three-necked flask, heat at 165°C for 1.2h, and weigh 0.672mg of CuCl 2 , 0.085g AgNO 3 , were dissolved in 5mL and 5mL of ethylene glycol solution, where CuCl 2 At a concentration of 0.001M, AgNO 3 The concentration of polyvinylpyrrolidone is 0.1M, and then 0.444g of polyvinylpyrrolidone with a molecular weight of 10000 is weighed and dissolved in 40mL of ethylene glycol, wherein the concentration of the ethylene glycol solution of polyvinylpyrrolidone is 0.1M (in terms of polyvinylpyrrolidone monomer), 5mL of CuCl 2 solution and 5 mL of AgNO 3 Pour the solution into a three-necked bottle, and finally add 40mL of polyvinylpyrrolidone solution dropwise to the reaction solution at a rate of 40mL / h. After the titration, stop heating, cool to room temperature, settle with acetone, and then wash with acetone. Centrifuge, and finally disperse the product in ethanol to obtai...

Embodiment 3

[0045] Preparation of conductive nanomaterials:

[0046] Measure 50mL of ethylene glycol solution into a three-necked flask, heat at 170°C for 4h, weigh 1.34mg of CuCl 2 , 1.7g AgNO 3 , respectively dissolved in 1mL and 10mL of ethylene glycol solution, where CuCl 2 At a concentration of 0.01M, AgNO 3 The concentration of polyvinylpyrrolidone is 1M, and then 6.66g of polyvinylpyrrolidone with a molecular weight of 1300000 is weighed and dissolved in 60mL of ethylene glycol, wherein the concentration of the ethylene glycol solution of polyvinylpyrrolidone is 1M (calculated as polyvinylpyrrolidone monomer), and 1mL CuCl 2 solution and 10 mL of AgNO 3 Pour the solution into a three-necked bottle, and finally add 60mL of polyvinylpyrrolidone solution dropwise to the reaction solution at a rate of 60mL / h. After the titration, stop heating, cool to room temperature, settle with acetone, and then wash with acetone. Centrifuge, and finally disperse the product in ethanol to obtai...

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Abstract

The invention discloses a preparation method of an electrochemical working electrode. The preparation method comprises the following steps: cleaning ITO conductive glass and carrying out hydrophilic treatment; then soaking the ITO conductive glass into a mixed solution of a metal salt solution and an alkali solution and drying the ITO conductive glass after being taken out; soaking the ITO conductive glass dried in the step S1 into a mixed solution of the metal salt solution and an organic amine solution, growing at the temperature of 60 to 200 DEG C for 5 to 25 hours until a layer of semiconductor material is attached to the ITO conductive glass; dropwise adding a conductive nano-material on one side on which the semiconductor material grows, of the ITO conductive glass, thus obtaining the electrochemical working electrode. When the working electrode is used in the process of detecting the blood glucose, an electrochemical signal generated by the blood glucose with lower concentrationcan be amplified, which has guiding significance for early diagnosis of diseases such as diabetes in biomedical field and the like.

Description

technical field [0001] The invention relates to the technical field of electrochemical detection of blood sugar, in particular to a preparation method of an electrochemical working electrode and a detection method of blood sugar. Background technique [0002] Inorganic nanomaterials are of great significance in human health and the environment. They have special physical and chemical properties. In addition, their large specific surface area and high active sites make them more and more widely used. They are now mostly used in chemical, biological, and pharmaceutical fields. . Hydrogen peroxide is an important molecule that is fundamental in clinical, industrial and environmental fields. Many reports have given the role of this molecule in some diseases. According to the concentration detection of hydrogen peroxide produced when blood sugar is catalyzed and decomposed, a blood sugar sensor can be made. This technology is used in blood sugar detection to understand blood sug...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/36G01N27/48
CPCG01N27/36G01N27/48
Inventor 郑冰陈欢欢吴琼霍峰蔚孔雪莹肖亚文李盛吴健生
Owner NANJING TECH UNIV