Polypeptide sequence, kit and method for detecting natural antibody against interleukin-2 receptor subunit a
A technology for detecting reagents and natural antibodies, applied in the field of immunology
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[0066] 1. Sample collection
[0067] The plasma samples of 23 patients with early non-small cell lung cancer (stage I squamous lung cancer and glandular lung cancer) before surgery and 199 healthy people were collected for detection of plasma AIL2RA natural antibody. All plasma samples were stored at minus 80 degrees (-80°C) before operation. It was verified that the storage time did not exceed two years, and there was no repeated freezing and thawing (no more than 3 times).
[0068] 2. Sample detection
[0069] The plasma samples were thawed at 4°C. The three polypeptide antigens (see Table 2) used in this experiment were synthesized by chemical solid phase with a purity of 95%. The specific steps were as follows:
[0070] (1) Before operation, each antigen was dissolved in 67% acetic acid into a 5.7 mg / ml stock solution, then mixed in equal volumes and stored in a -20°C refrigerator.
[0071](2) At the beginning of the operation, at first the three kinds of antigens were d...
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