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Sunflower flower disc general flavone and extraction process and application thereof

An extraction process and sunflower technology, applied in the field of biomedicine, can solve the problems associated with adverse reactions, treating the symptoms but not the root cause, etc.

Inactive Publication Date: 2018-08-24
长春市万象鹰豪商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, with the changes in people's lifestyles, a large number of high-purine foods have been ingested, leading to an increase in the incidence of gouty arthritis year by year. , safe and effective anti-gout drugs are getting more and more attention

Method used

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  • Sunflower flower disc general flavone and extraction process and application thereof
  • Sunflower flower disc general flavone and extraction process and application thereof
  • Sunflower flower disc general flavone and extraction process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The extraction of total flavonoids of sunflower disc of embodiment 1

[0053] 1. Drawing of standard curve

[0054] Accurately weigh 5.0 mg of standard rutin that was dried at 105°C to a constant mass, place it in a small beaker for ultrasonic dissolution, and make 10 mL of rutin standard stock solution (0.5 mg / mL). After diluting the rutin standard stock solution into the rutin standard application solution of different mass concentrations, according to the NaNO 2 -Al(NO 3 ) 3 method, adding 5% (mass fraction) NaNO 2 Solution 1.0mL, let stand for 6min, then add 10% (mass fraction) Al(NO 3 ) 3Solution 1.0mL, mix well, let stand for 6min. Add 8.0 mL of 4% (mass fraction) NaOH solution and let stand for 15 min. Add distilled water to make up to volume, and mix thoroughly to obtain rutin standard control solutions with different mass concentrations. Measure the optical density value of each concentration standard substance under the absorption wavelength of 510nm, ...

Embodiment 2

[0075] Example 2 Therapeutic effect of total flavonoids from sunflower discs on gouty arthritis

[0076] 1. Experimental animal grouping method

[0077] The experimental animals were 80 clean-grade male Wister rats, weighing 180±20 g, purchased from Changchun Yisi Experimental Animal Technology Co., Ltd., animal certificate number SCKX (Ji)-2016-0003. Adaptive culture was carried out in the first week, and then they were randomly divided into 4 groups (20 rats in each group), and the treatment was as follows:

[0078] Normal control group: continuous intragastric administration of normal saline from the second week to the third week (once a day, the volume of a single intragastric administration is 12 mL of normal saline per kilogram of body weight). Inject sterile normal saline (injection dose: 0.2mL sterile normal saline per mouse).

[0079] Model group: From the 2nd week to the 3rd week, the normal saline was administered continuously (the method was the same as that of t...

Embodiment 3

[0100] The analgesic effect of embodiment 3 total flavonoids of sunflower disc

[0101] 1. The effect of total flavonoids of sunflower disc on the writhing response of mice induced by acetic acid

[0102] The experimental animals were 80 clean-grade male mice, weighing 20±2g, purchased from Changchun Yisi Experimental Animal Technology Co., Ltd., animal certificate number SCKX (Ji)-2016-0003. Adaptive culture was carried out in the first week, and then they were randomly divided into 4 groups (20 rats in each group), and the treatment was as follows:

[0103] Normal control group: intragastric administration of normal saline for 7 consecutive days in the second week (once a day, the volume of a single intragastric administration was 12 mL of normal saline per kilogram of body weight), and 1 hour after the last administration, the mice were intraperitoneally injected with normal saline ( The injection dose is: 0.1 mL of normal saline per 10 g of body weight).

[0104] Model g...

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Abstract

The invention relates to the field of biological medicine, in particular to sunflower flower disc general flavone and an extraction process and application thereof. The extraction process comprises the steps that sunflower flower disc powder is accurately weighed to achieve the constant mass, an ethanol aqueous solution with the volume fraction of 40% to 70% is adopted, the solid-liquid ratio ranges from 1:10 to 1:40, the extraction time ranges from 1 h to 4 h, and extraction is carried out 1-4 times; extracting solutions are mixed, and a crude product of sunflower flower disc general flavoneis obtained; the crude product of sunflower flower disc general flavone is purified with D101 macroporous resin, and the mass ratio of the maxium sampling amount to the D101 macroporous resin is 7:10,an ethanol aqueous solution with the volume fraction of 50% is adopted as an eluent, and the flow velocity is 2.5 mL / min; the eluant is collected, ethanol is recycled, freeze-drying is carried out after vacuum concentration is carried out, and sunflower flower disc general flavone extract is obtained. Sunflower flower disc general flavone extracted through the optimum extraction process has the functions of treating arthrocele, easing pain, treating gouty arthritis and the like.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a sunflower disc total flavonoids and its extraction process and application. Background technique [0002] Gouty arthritis (GA), also known as uric acid arthritis, is an increase in blood uric acid or a decrease in uric acid excretion in the body caused by purine metabolism disorders, resulting in urate deposition in the joint cavity, synovial bursa, cartilage, bone and Acute rheumatic lesions arising in other tissues. During the onset of gout, the sodium urate crystals released in the synovial tissue and articular cartilage are phagocytized by the leukocytes in the joint fluid, and when the leukocytes are necrotic, they disintegrate and release proteases and inflammatory factors, which promote the increase of the leukocyte content, so there will be more phagocytosis Leukocytes containing urate crystals rupture one after another, releasing enzymes and inflammatory component...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P19/06A61P19/02A61P29/00A23L33/00A61K133/00
CPCA61K36/28A23L33/00A23V2002/00A61K2236/333A61K2236/51A61K2236/55A61P19/02A61P19/06A61P29/00A23V2200/30
Inventor 李田野
Owner 长春市万象鹰豪商贸有限公司
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