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A kind of purification method of salinomycin sodium

The technology of a salinomycin sodium and a purification method is applied in the purification field of salinomycin sodium and salinomycin sodium, which can solve the problems of inconvenient automatic control, complicated process route, many impurities in finished products, etc., and is beneficial to occupational health, Improve preparation efficiency and high content

Active Publication Date: 2019-04-09
内蒙古拜克生物有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process route in the above invention is complex, labor-intensive, inconvenient for automatic control, the amount of alkali used is too large, and the finished product has many impurities; and the use of methanol will cause harm to the human body.

Method used

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  • A kind of purification method of salinomycin sodium
  • A kind of purification method of salinomycin sodium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A kind of purification method of salinomycin sodium of embodiment 1

[0049] The specific steps are:

[0050] (1) Flocculation: get 100 liters of salinomycin fermentation liquid, after adjusting pH to 5 with hydrochloric acid, add polyacrylamide 200g in the acidified salinomycin fermentation liquid, after stirring at room temperature for 30 minutes, add calcium carbonate 200g, After stirring evenly, let it stand for 2 hours, then remove the supernatant and collect the lower floc;

[0051] (2) Alkali adjustment: adjust the pH value of the lower floor floc obtained in step (1) to 7 with sodium hydroxide, and mix well to obtain mixture A;

[0052] (3) Extraction: Add ethanol to the mixture A obtained in step (2), and extract while stirring. The volume ratio of the added ethanol to mixture A is 0.5:1, and the extraction time is 2 hours. The extraction temperature is 100r / min, and the extraction temperature is 25°C; the obtained extraction solution is concentrated to 40% o...

Embodiment 2

[0056] A kind of purification method of salinomycin sodium of embodiment 2

[0057] The specific steps are:

[0058] (1) Flocculation: Take 100 liters of salinomycin fermentation broth, adjust the pH to 6.5 with sulfuric acid, add 300 g of polyethylene oxide to the acidified salinomycin fermentation broth, stir at room temperature for 30 minutes, and then add 400 g of diatomaceous earth , after stirring evenly, let it stand for 2 hours, then remove the supernatant, and collect the lower floc;

[0059] (2) Alkali adjustment: adjust the pH value of the lower floor floc obtained in step (1) to 9 with sodium hydroxide, and mix well to obtain mixture A;

[0060] (3) Extraction: Add ethanol to the mixture A obtained in step (2), and extract while stirring. The volume ratio of the added ethanol to mixture A is 1.5:1, and the extraction time is 6 hours. The extraction temperature is 400r / min, and the extraction temperature is 40°C; the obtained extraction solution is concentrated to...

Embodiment 3

[0064] A kind of purification method of salinomycin sodium of embodiment 3

[0065] The specific steps are:

[0066] (1) Flocculation: take 100 liters of salinomycin fermentation broth, adjust the pH to 5.5 with hydrochloric acid, add 220 g of lignin to the acidified salinomycin fermentation broth, stir at room temperature for 30 minutes, add 250 g of perlite, and stir After uniformity, let it stand for 2 hours, then remove the supernatant and collect the lower floc;

[0067] (2) Alkali adjustment: adjust the pH value of the lower floor floc obtained in step (1) to 7.5 with sodium hydroxide, and stir to obtain mixture A;

[0068] (3) Extraction: Add ethanol to the mixture A obtained in step (2), and extract while stirring, the volume ratio of the added ethanol and mixture A is 1.1:1, the time of extraction is 3 hours, the speed of stirring The extraction temperature is 300r / min, and the extraction temperature is 38°C; the obtained extraction solution is concentrated to 25% o...

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Abstract

The invention relates to a purification method of salinomycin sodium. The purification method of the salinomycin sodium, disclosed by the invention, takes salinomycin fermentation liquid as a raw material and comprises the following steps: firstly, flocculating; then enabling salinomycin to form sodium salt through an alkali adjusting method; then preparing the salinomycin sodium through a seriesof methods including extraction, de-coloring, crystallization and recrystallization. By adopting the preparation method provided by the invention, the yield and purity of the salinomycin sodium are improved; ethanol is used as an extraction agent and the content of the extracted salinomycin sodium is high; the utilized ethanol has small damages to human bodies; a gradient cooling and crystallization method is adopted and the yield of the product provided by the invention is improved through selecting suitable gradient temperature; the production operation is convenient and the temperature is easy to control.

Description

technical field [0001] The invention relates to a method for purifying salinomycin sodium, in particular to a method for purifying salinomycin sodium from fermentation liquid, and belongs to the technical field of antibiotic production. Background technique [0002] Salinomycin, also known as salinomycin, coccidian powder, and Yousu, is a polyether ionophore-type animal-specific antibiotic produced by Streptomyces albicans. Its chemical structure is C 42 h 70 o 11 , in practical application, commonly used its sodium salt, namely salinomycin sodium, molecular formula is C 42 h 69 o 11 Na. Salinomycin sodium (SAL-Na) is a typical ionophore antibiotic. It is a white crystalline powder, easily soluble in methanol, ethanol, acetone, ether, chloroform, dimethyl sulfoxide, slightly soluble in n-hexane, almost insoluble in Water, stable in neutral or alkaline environment, not deactivated at high temperature of 120°C, easily deactivated in acidic medium. [0003] Salinomycin h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/20
CPCC07D493/20
Inventor 叶朋钟迎东周彦乐杨爱华程兆全
Owner 内蒙古拜克生物有限公司