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Preparation method of high-content icariin extract

The technology of icariin and extract is applied in the field of preparation of high-content icariin extract, which can solve the problems of large solvent, high cost, complicated operation, etc. simple effect

Inactive Publication Date: 2018-08-24
GANZHOU H L K J BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nowadays, the icariin extract initially obtained by conventional technological means not only has low purity of icariin, but also relatively low yield, so that the overall yield of icariin prepared by Epimedium medicinal material is relatively low. low; and for the production of high-content icariin products, it is necessary to carry out other extraction steps on this basis, and these additional processes are not only complicated to operate, but also consume a large amount of solvent, resulting in higher cost. At the same time, it will pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Take 10kg of Epimedium medicinal material (purchased directly in the corresponding traditional Chinese medicine pharmacy) and grind it to 60 meshes, then extract it with 80kg of water and filter it to obtain an extract, and concentrate the extract to obtain a concentrate 1; Add concentrated solution-2 times the volume of mass concentration of 90% ethanol solution to concentrated solution-1, stir at room temperature for 2 hours, and let stand for 8 hours, then filter to obtain filtrate-1 and filter cake; filtrate-1 Remove the ethanol wherein by distillation, and add the HL-5 enzyme (HL-5 enzyme is the enzyme that cellulase and glucosidase mixes) of filtrate one volume 1% after distillation, and static at 40 ℃ of temperature Stand for 12 hours; extract the standing solution 4 times with a mixed solvent with a mass ratio of n-butanol and ethyl acetate of 3:7 to obtain an extraction supernatant, and remove the mixed solvent in the extraction supernatant by distillation , to...

Embodiment 2

[0030]Take 10kg of Epimedium medicinal material (purchased directly in the corresponding traditional Chinese medicine pharmacy) and grind it to 70 meshes, then extract and filter it with 100kg of water to obtain an extract, and concentrate the extract to obtain a concentrate 1; Add concentrated solution-2.2 times volume of mass concentration of 95% ethanol solution in concentrated solution-1, stir at normal temperature for 0.5 hours, and let stand for 5 hours, then filter again to obtain filtrate-1 and filter cake; filtrate-1 Remove the ethanol wherein by distillation, and add the HL-5 enzyme (HL-5 enzyme is the enzyme that cellulase and glucosidase mixes) of filtrate-volume 1.5% after distillation, and static at 45 ℃ of temperature Stand for 14 hours; extract the standing solution 5 times with a mixed solvent with a mass ratio of n-butanol and ethyl acetate of 3:7 to obtain an extraction supernatant, and remove the mixed solvent in the extraction supernatant by distillation ,...

Embodiment 3

[0032] Take 10kg of Epimedium medicinal material (purchased directly in the corresponding traditional Chinese medicine pharmacy) and grind it to 80 meshes, then extract it with 120kg of water and filter it to obtain an extract, and concentrate the extract to obtain a concentrate 1; Add concentrated solution-1.8 times volume of mass concentration of 85% ethanol solution in concentrated solution-1, stir at normal temperature for 1.2 hours, and let stand for 6.5 hours, then filter again to obtain filtrate-1 and filter cake; filtrate-1 Remove the ethanol wherein by distillation, and add the HL-5 enzyme (HL-5 enzyme is the enzyme that cellulase and glucosidase mixes) of filtrate-volume 2% after distillation, and static at 55 ℃ of temperature Stand for 10 hours; extract the standing solution 3 times with a mixed solvent with a mass ratio of n-butanol and ethyl acetate of 3:7 to obtain an extraction supernatant, and remove the mixed solvent in the extraction supernatant by distillatio...

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PUM

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Abstract

The invention discloses a preparation method of a high-content icariin extract. The preparation method comprises the following specific steps: crushing a herba epimedii medicinal material, extractingwith water, filtering to obtain an extracting solution and concentrating the extracting solution; then adding an ethanol solution in the extracting solution and standing; filtering, distilling to remove ethanol, adding an HL-5 enzyme and standing; extracting with a mixed solvent and removing the mixed solvent through distillation; absorbing through macro-porous resin and resolving after impurity removal; adding activated carbon, filtering after a period of time and standing to precipitate a crystal; and filtering, washing and drying the crystal to obtain an icariin extract finished product. The preparation method disclosed by the invention has the benefits that the extraction, the transformation and the purification of icariin are integrated, so that the high-content icariin extract is obtained through primary processing, and the content of the icariin can reach above 98 percent; and besides, the preparation method is simple to operate, low in cost, completely free of pollution and capable of being used on a large scale.

Description

technical field [0001] The invention belongs to the field of plant component extraction, in particular to a preparation method of a high-content icariin extract. Background technique [0002] Epimedium is a common traditional Chinese medicine in traditional Chinese medicine. Its main medicinal ingredient is icariin. Icariin can increase the blood flow of the cardiovascular and cerebrovascular, promote hematopoietic function, immune function and bone metabolism. It also has the functions of invigorating the kidney and strengthening yang, anti-aging, and anti-tumor. Therefore, icariin is widely used in modern medicine. Therefore, the extraction of ingredients in Epimedium medicinal materials today is mainly the extraction of icariin. [0003] Nowadays, the icariin extract obtained preliminarily by conventional technological means not only has low purity of icariin, but also relatively low yield, so that the overall yield of icariin prepared by Epimedium medicinal material is r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 胡晓雁李伟管兰芳钟祥禄胡君惠陈曙平
Owner GANZHOU H L K J BIOTECH CO LTD