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A device for automatic cell purification and freezing

A cryopreservation and cell technology, applied in biochemical cleaning devices, preservation of human or animal bodies, enzymology/microbiology devices, etc. It can solve the problems of frequent manual operation steps, affecting biological effects, and easy damage to cells. , to achieve the effect of saving manpower, saving physical and material resources, and avoiding the loss of cells

Active Publication Date: 2022-05-13
天晴干细胞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to purify stem cells or other cells from liquid biological products and freeze them automatically, so as to overcome the defects of frequent manual operation steps when separating samples, easily lead to cell contamination, easily damaged cells and affect biological effects, etc., and provide A cell automatic purification and freezing device that reduces open operation and avoids sample contamination, so as to improve preparation efficiency, reduce sample contamination and cell viability

Method used

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  • A device for automatic cell purification and freezing
  • A device for automatic cell purification and freezing
  • A device for automatic cell purification and freezing

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specific Embodiment approach 1

[0019] Specific embodiment 1: The automatic cell purification and freezing storage device in this embodiment includes a purification freezing storage box 1, a liquid bag holder 5, a shut-off valve 7, a peristaltic pump 8, a suspension separation device 9 and a push injection device 10, and a purification freezing storage box 1 The left side of the electric control purification area is the electronic control purification area, the right side of the purification cryopreservation box 1 is the freezing storage area, the upper part of the electronic control purification area is a display screen 6, and a plurality of peristaltic pumps 8 and A suspension separation device 9, the lower part of the electronically controlled purification area is provided with a push injection device 10, a plurality of shut-off valves 7 are arranged on the side box wall of the electronically controlled purification area, and there is a connection between the electronically controlled purification area and ...

specific Embodiment approach 2

[0023] Embodiment 2: This embodiment differs from Embodiment 1 in that a weighing sensor is provided on the pre-mixing platform 3 .

[0024] In this embodiment, the weight of the cell fluid in the cryopreservation bag is measured by a load cell, and the heat sealing device is controlled to heat seal the infusion pipeline through a given parameter measurement to separate the pipeline.

specific Embodiment approach 3

[0025] Embodiment 3: This embodiment is different from Embodiment 1 or Embodiment 2 in that a temperature sensor is provided in the freezing storage area.

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Abstract

An automatic cell purification and freezing device, the invention belongs to the field of biotechnology, which solves the defects of frequent manual operation steps when separating samples, which easily leads to cell pollution, easily damaged cells and affects biological effects. The cell automatic purification and freezing device includes a purification freezing box, a liquid bag rack, a stop valve, a peristaltic pump, a suspension separation device, and a push injection device. The right side is the cryopreservation area. Multiple peristaltic pumps and a suspension separation device are installed on the middle wall of the electronically controlled purification area. The lower part of the electronically controlled purification area is equipped with a push injection device. Cells are hung on the liquid bag rack. The storage bag, the infusion tube connected to the bottom of the cell storage bag is clamped in the peristaltic pump through the shut-off valve, and the cryopreservation bag and heat sealing device are placed on the pre-mixing platform in the box of the cryopreservation area. The automatic cell purification and freezing storage device of the present invention realizes multifunctional automation, improves operation accuracy and reduces the risk of biological contamination.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a control device for automatic cell purification and programmed freezing, which is used for cell separation, extraction and programmed freezing. Background technique [0002] With the development of biotherapy technology, cell purification technology is gradually optimized and developed, from the initial traditional centrifuge through the control of the corresponding speed to achieve the purpose of layered separation of cells, to the current development of a complete set of sterile pipelines through the centrifuge cup at high speed Centrifugation separates the cell components to the edge, thereby separating the required components, but the separation speed is slow, the noise is large, the cell loss is high, the human intervention is more and the preparation stability is poor. [0003] Program-controlled cooling technology is to use temperature control technology and liqui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/36C12M1/34A01N1/02
CPCA01N1/0252C12M23/14C12M41/12C12M41/36
Inventor 谢兴琴张宇张怡刘艳青
Owner 天晴干细胞股份有限公司
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