A culture medium for high-efficiency bacterial cellulose production
A technology of bacterial cellulose and culture medium, applied in the field of culture medium for high-efficiency bacterial cellulose production, can solve the problems of fermentation medium that have not been seen yet, and achieve the effects of increasing bacterial cellulose production, good application value, and reducing processing costs
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Embodiment 1
[0027] The culture medium for bacterial cellulose preparation provided by the tobacco extract provided in this example is prepared through the following steps.
[0028] (1) To prepare a tobacco extract, take the tobacco raw material, add water with 6 times the mass of the tobacco raw material, extract at 70 °C for 60 min, collect the extract and cool it to room temperature naturally for use;
[0029] (2) Enzymatic hydrolysis treatment of the extract, adding amylase and protease to the extract in step (1) for enzymatic hydrolysis, specifically: in each liter of tobacco extract, the amount of amylase and protease added is equal 100kU, enzymatic hydrolysis for 60 min, boiling for inactivation;
[0030] (3) Adding nutrients, centrifuging the extract after enzymatic hydrolysis treatment in step (2), taking the supernatant, in terms of mass-volume ratio, each milliliter of tobacco extract contains: 0.15% MgSO 4 , 0.01% FeSO 4 , 5.0% peptone, 5.0% yeast extract, add 4% absolute eth...
Embodiment 2
[0038] Compared with Example 1, the bacterial cellulose preparation medium prepared by using tobacco extract provided in this example omits the steps of enzymatic hydrolysis and nutrient addition, and only uses the tobacco extract after extraction. Used as a culture medium.
[0039] When producing bacterial cellulose by fermentation, the specific treatment process is the same as that in Example 1.
[0040] The bacterial cellulose yields prepared in Examples 1 and 2 were compared, and the results were shown in the following table:
[0041] .
[0042] From the comparison of the above data, it can be seen that although the tobacco extract can also be directly used for the production of bacterial cellulose, after further enzymatic hydrolysis treatment and nutritional adjustment, the yield of bacterial cellulose can be greatly increased, showing a good application effect. .
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