Applications of corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait

A technology of maize rough dwarf disease and molecular marker, applied in the directions of application, chemical instruments and methods, botanical equipment and methods, etc., can solve the problems of single source of maize rough dwarf disease resistance, polluting the environment, increasing costs, etc.

Active Publication Date: 2018-08-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the prevention and control of rough dwarf disease mainly depends on two methods: chemical control and adjustment of sowing date: chemical control pollutes the environment and increases costs; adjustment of sowing date has great limitations, which not only wastes light and temperature resources, but also easily suffers from later natural disasters. disaste

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  • Applications of corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait
  • Applications of corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait
  • Applications of corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait

Examples

Experimental program
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Effect test

Embodiment 1

[0116] Example 1, the application of maize molecular markers in the identification of maize rough dwarf resistance traits

[0117] The corn molecular marker provided in this example is a DNA molecule obtained by amplifying primer pair A1; A1 is composed of two single-stranded DNAs (named P1 and P2 respectively) shown in sequence 7 and sequence 8 in the sequence listing primer pair. A1 can amplify the DNA fragment shown in the 7036-10712 position of the sequence 5 or the DNA fragment shown in the 7033-8161 position of the sequence 6 by using the corn genome as a template. Compared with the DNA fragment shown at 7036-10712 of Sequence 5, the DNA fragment at 7033-8161 of Sequence 6 lacks 7418-9965 of Sequence 5.

[0118] 1. Correlation analysis between maize molecular markers and maize rough dwarf resistance traits in existing inbred lines

[0119] 1.1. Corn to be tested

[0120] The test objects were 619 maize inbred lines from different sources (Table 1).

[0121] 1.2. Geno...

Embodiment 2

[0164] Example 2, the application of maize molecular markers in regulating the resistance traits of maize rough dwarf disease

[0165] The genomic DNA of the complete gene containing the DNA fragment shown in the 7036-10712th position of sequence 5 derived from the disease-resistant maize inbred line X178 is named ZmGDI1 gene, the protein encoded by the ZmGDI1 gene is named ZmGDI1, and the ZmGDI1 gene is named ZmGDI1. The CDS sequence of ZmGDI1 is named as the ZmGDI1 coding gene, the sequence of the ZmGDI1 gene is sequence 5 in the sequence listing, the sequence of ZmGDI1 is sequence 3 in the sequence listing, and the sequence of the ZmGDI1 coding gene is 136-1428 of sequence 1 in the sequence listing. From the results of Example 1, it can be seen that the ZmGDI1 gene is a recessive rough dwarf disease resistance gene.

[0166] The genomic DNA of the complete gene containing the DNA fragment shown in the 7033-8161 position of sequence 6 derived from the corn inbred line B73 su...

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Abstract

The invention discloses applications of a corn molecular marker in identification and regulation of maize rough dwarf disease resistance trait. According to the present invention, the corn molecular marker is a DNA molecule obtained by amplifying with a primer pair A1 by using the genomic DNA of corn as a template, wherein the DNA fragment containing the sites 7418-9965 of a sequence 5 can be obtained through amplifying with the A1 by using the genomic DNA of corn as the template; and the experiment results prove that the corn molecular marker is significantly correlated with the maize rough dwarf disease resistance trait, and can be used for identifying the maize rough dwarf disease resistance trait, wherein the ZmGDI2 gene with no the sites 7418-9965 of the sequence 5 can significantly increase the disease susceptibility of corn to rough dwarf disease, and the ZmGDI1 gene containing the sites 7418-9965 of the sequence 5 can significantly increase the disease resistance of corn to rough dwarf disease, such that the disease resistance of corn to rough dwarf disease can be regulated by using the ZmGDI2 gene and the ZmGDI1 gene.

Description

technical field [0001] The invention relates to the application of maize molecular markers in identifying and regulating maize rough dwarf resistance traits in the field of biotechnology. Background technique [0002] Maize (Zea Mays L.) is the largest food crop in my country and plays an important role in China's energy and food security. Maize rough dwarf disease is a worldwide viral disease, which seriously endangers the production of maize. Since it was discovered in my country's Xinjiang and Gansu regions in 1954, maize rough dwarf disease has broken out in a large area in various production areas of my country, especially in the Huanghuaihai region, causing large-scale destruction or extinction of production, posing a serious threat to corn production. In 2007, nearly 53,300 hm in Shandong Province 2 Maize production was extinct due to rough dwarf disease. In 2008, Shandong Province suffered from corn rough dwarf disease, which was rare in history. The occurrence are...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895A01H1/04C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCA01H1/04C07K14/415C12N15/8283C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐明良刘保申刘庆彩邓穗宁陶永富
Owner CHINA AGRI UNIV
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