Application of pirna-54265 in the diagnosis, treatment and prognosis evaluation of colorectal cancer

A technology of pirna-54265, 1. pirna-54265, which is applied in the field of diagnosis, treatment and prognosis evaluation, and can solve problems affecting the treatment effect, etc.

Active Publication Date: 2021-01-15
SUN YAT SEN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In addition, the prognosis judgment of colorectal cancer treatment and the selection of individualized precise treatment plan are also crucial, and the differences of different individuals will significantly affect the treatment effect

Method used

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  • Application of pirna-54265 in the diagnosis, treatment and prognosis evaluation of colorectal cancer
  • Application of pirna-54265 in the diagnosis, treatment and prognosis evaluation of colorectal cancer
  • Application of pirna-54265 in the diagnosis, treatment and prognosis evaluation of colorectal cancer

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. Experimental method

[0041] Extracted and analyzed TCGA and GEO expression profile data, obtained the top 20 piRNAs with the highest expression in colorectal cancer, designed specific primers for the top 20 piRNAs, and tested them in the cancer tissues and tumors of 110 colorectal cancer patients from Guangzhou. qPCR expression levels were detected in adjacent tissue samples. The identified piR-54265 was verified again by qPCR in 108 colorectal cancer patients with colorectal cancer and paracancerous tissue samples from Suzhou. Bioinformatics analysis was performed on gene copy number variation and DNA methylation modification data of COAD samples in TCGA.

[0042] 2. Experimental results

[0043] Such as figure 1 As shown in panels a and b, only the expression of piRNA-54265 has a significant statistical difference between colorectal cancer and paracancerous tissues, and piRNA-54265 is highly expressed in cancer tissues, and samples from two clinical centers (Gu...

Embodiment 2

[0051] 1. Experimental method

[0052] Use SPSS (19.0) to analyze the expression level of piR-54265 in cancer tissue and the prognosis of colorectal cancer patients (such as the aforementioned 110 patients with colorectal cancer undergoing chemotherapy in Guangzhou and 108 patients in Suzhou) for survival analysis.

[0053] 2. Experimental results

[0054] The expression level of piR-54265 in cancer tissue is related to the survival and prognosis of colorectal cancer patients. The prognosis of colorectal cancer patients with high expression of piR-54265 is poor, and their 5-year overall survival and 5-year progression-free survival time are shorter. The results of the two clinical sample centers are consistent (such as figure 2 shown).

Embodiment 3

[0056] 1. Experimental method

[0057] Collect the pre-treatment serum samples corresponding to the colorectal cancer cases used before, and randomly select 4 serum samples for Northern Blot test to directly determine whether there is serum piR-54265 expression; and then perform RNA extraction and RT-qPCR on all samples The expression level of piR-54265 was detected, and the correlation analysis was performed between serum piR-54265 and cancer tissue piR-54265 levels. Survival analysis was performed on the serum piR-54265 level and the corresponding patient prognosis. Apply to collect 111 normal human serum samples, perform RNA extraction and RT-qPCR to detect the expression level of piR-54265, and conduct unpaired Student’s t-test statistical analysis with the serum piR-54265 level of colorectal cancer patients.

[0058] 2. Experimental results

[0059] Such as image 3 As shown in panel a, the expression of piRNA-54265 can be detected in the serum of colorectal cancer pat...

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Abstract

The invention discloses the application of piRNA-54265 in the diagnosis, treatment and prognosis evaluation of colorectal cancer. Based on the main research findings of the present invention, piRNA-54265 can be used as a diagnostic marker for colorectal cancer, specifically indicating the chemotherapy sensitivity and prognosis of colorectal cancer patients, and contributes to more accurate individualized selection of clinical treatment plans and improved prognosis ; It can also be used as a therapeutic target for colorectal cancer, and can be used to prepare colorectal cancer therapeutic drugs, especially piRNA-54265 expression inhibitors or knockout reagents can be used as colorectal cancer therapeutic drugs, and can be used as an auxiliary for improving the effect of colorectal cancer chemotherapy Drugs capable of inhibiting the proliferation, invasion, metastasis and / or colonization of colorectal cancer cells. Therefore, piRNA‑54265 is a very potential and promising specific molecular marker for colorectal cancer, which can be easily detected in blood samples and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of piRNA-54265 in the diagnosis, treatment and prognosis evaluation of colorectal cancer. Background technique [0002] Colorectal cancer is a common gastrointestinal tumor in the world. It ranks first among all kinds of malignant tumors in my country and is one of the main causes of death worldwide. Difficulties. The diagnosis of colorectal cancer is of great significance for the treatment. [0003] Chemotherapy regimens for colorectal cancer are also plagued by tumor drug resistance. According to the estimates of the American Cancer Society, more than 90% of tumor patients died of different degrees of drug resistance. The problem of tumor drug resistance has become a key factor for the success of cancer chemotherapy. The chemosensitivity of patients has an important influence on the effect of chemotherapy. [0004] In addition, the progno...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCA61K45/00C12Q1/6886C12Q2600/178C12Q2600/158A61P35/00
Inventor 林东昕郑健麦冬梅谭莉萍
Owner SUN YAT SEN UNIV
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