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A test strip for detecting onchocerciasis metabolite natog in urine and its preparation method and test card

A technology of onchocerciasis and test strips, which is applied in the biological field, can solve the problems of infectious disease expansion, river blindness, etc., and achieve the effects of simple preparation method, low cost and convenient operation

Active Publication Date: 2021-03-26
江苏维尔生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 2002, the OCP program came to an end, but the problem of river blindness has not been completely solved, so continuous monitoring is still needed in the future to prevent the epidemic from spreading again
[0008] At present, the detection of NATOG in the domestic market is still in a blank period, therefore, it is very important to develop a test strip for detecting NATOG in urine

Method used

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  • A test strip for detecting onchocerciasis metabolite natog in urine and its preparation method and test card
  • A test strip for detecting onchocerciasis metabolite natog in urine and its preparation method and test card
  • A test strip for detecting onchocerciasis metabolite natog in urine and its preparation method and test card

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Embodiment 1

[0059] The test strip of the present embodiment detects onchocerciasis metabolite NATOG in urine, such as figure 1 As shown, including PVC board 5, sample pad 1, gold standard pad 2, NC film 3, absorbent paper 4, sample pad 1, gold standard pad 2, NC film 3, and absorbent paper 4 are pasted on the PVC board in sequence; Pad 2 is coated with NATOG antibody-colloidal gold marker, rabbit IgG colloidal gold marker; NC membrane 3 is coated with NATOG-BSA hapten 6, goat anti-rabbit IgG7, NATOG-BSA hapten is close to the sample pad, sheep The anti-rabbit IgG is close to the absorbent paper; the overlap between the sample pad and the gold standard pad, between the gold standard pad and the NC membrane, and between the NC membrane and the absorbent paper is 2 mm.

[0060] The preparation method of the test strip for detecting the onchocerciasis metabolite NATOG in the urine of the present embodiment comprises the following steps:

[0061] 1) Soak the glass fiber with NATOG antibody-co...

Embodiment 2

[0074] The test strip for detecting the onchocerciasis metabolite NATOG in the urine of this embodiment includes a PVC plate, a sample pad, a gold standard pad, an NC film, and absorbent paper, and the sample pad, the gold standard pad, the NC film, and the absorbent paper are pasted in sequence On PVC plate; NATOG antibody-colloidal gold marker and rabbit IgG colloidal gold marker are coated on the gold label pad; NATOG-BSA hapten and goat anti-rabbit IgG are coated on the NC membrane, and the NATOG-BSA hapten is close to the sample Pad, goat anti-rabbit IgG is close to the absorbent paper; between the sample pad and the gold standard pad, between the gold standard pad and the NC membrane, and between the NC membrane and the absorbent paper are all overlapped by 1mm.

[0075] The preparation method of the test strip for detecting the onchocerciasis metabolite NATOG in the urine of the present embodiment comprises the following steps:

[0076] 1) Soak the glass fiber with NATO...

Embodiment 3

[0089] The test strip for detecting the onchocerciasis metabolite NATOG in the urine of this embodiment includes a PVC plate, a sample pad, a gold standard pad, an NC film, and absorbent paper, and the sample pad, the gold standard pad, the NC film, and the absorbent paper are pasted in sequence On PVC plate; NATOG antibody-colloidal gold marker and rabbit IgG colloidal gold marker are coated on the gold label pad; NATOG-BSA hapten and goat anti-rabbit IgG are coated on the NC membrane, and the NATOG-BSA hapten is close to the sample Pad, goat anti-rabbit IgG is close to the absorbent paper; between the sample pad and the gold standard pad, between the gold standard pad and the NC membrane, and between the NC membrane and the absorbent paper are all overlapped by 1.5mm.

[0090] The preparation method of the test strip for detecting the onchocerciasis metabolite NATOG in the urine of the present embodiment comprises the following steps:

[0091] 1) Soak the glass fiber with NA...

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Abstract

The invention relates to a test strip for detecting an onchocerca volvulus metabolite NATOG (nacetyltyramine-O, beta-glucuronide) in urine and a preparation method and a test card thereof, and belongsto the technical field of biology. The test strip for detecting the onchocerca volvulus metabolite NATOG (nacetyltyramine-O, beta-glucuronide) in the urine comprises a bottom plate, a sample pad, a gold label pad, an NC membrane and absorbent paper, wherein the sample pad, the gold label pad, the NC membrane and the absorbent paper are sequentially adhered to the bottom plate; the gold label padis coated with an NATOG antibody-colloidal gold label and a rabbit IgG colloidal gold label; the NC membrane is coated with an NATOG-BSA hapten and goat anti-rabbit IgG respectively. The test strip for detecting the NATOG in the urine completes urine collection and delivery through the sample pad, and is prepared by a colloidal gold technology and an immunochromatography principle which are combined to achieve one-step rapid detection of the NATOG in the urine.

Description

technical field [0001] The invention relates to a test strip for detecting onchocerciasis metabolite NATOG in urine, a preparation method and a test card, and belongs to the field of biotechnology. Background technique [0002] Onchocerciasis is a parasitic disease characterized by lichenoid dermatitis, subcutaneous nodules and visual impairment caused by Onchocercia volvulus parasitizing on human skin, subcutaneous tissue and eyes, also known as river blindness or blind filariasis. Widely prevalent in Africa and tropical America, it can cause blindness in 5% to 20% of adults in endemic areas. Worldwide more than 37 million people are infected with the disease, which is transmitted by the gnat (black fly). [0003] In 1974, the first Onchocerciasis Control Program (OCP) for river blindness helped 11 countries spend $30 million to spray rivers with the larvicide larvicide to control gnat populations. The program mobilizes the World Health Organization, the World Bank, the U...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/94
CPCG01N33/9406
Inventor 王慧英
Owner 江苏维尔生物科技有限公司
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