Four-dimensional fluorescence resonance energy transfer efficiency visual microscopic analysis system and method
A technology of fluorescence resonance energy and transfer efficiency, which is applied in the direction of material analysis, analysis of materials, fluorescence/phosphorescence, etc. by optical means, which can solve the problem of not having the effect of real-time detection and observation of microscopic optics, and achieve the simplification of detection methods and Intuitive, easy-to-use, spectrally optimized results
Active Publication Date: 2021-08-13
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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[0004] At present, the detection of fluorescence resonance energy transfer efficiency is based on the calculation and analysis results of microscopic digital images of laser confocal or fluorescence imaging. Image analysis and calculation processing software can be used to obtain relevant two-dimensional (plane), three-dimensional (stereo) and four-dimensional (stereo and time) detection digital pictures, and quantify experimental results, which do not have the effect of microscopic optical real-time detection and observation
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[0046]The present invention is described in further detail now in conjunction with accompanying drawing.
[0047] Fluorescence resonance energy transfer imaging is realized by observing different emission spectra or polarized light intensity of donor and acceptor fluorescent molecules. It belongs to two imaging (or two digital pictures). This imaging can use different spectra or The detection modes of different polarization directions (or anisotropy) of the spectrum are obtained, and the use of a spectroscope can realize one-time imaging. The four-dimensional fluorescence resonance energy transfer efficiency visual microscopic analysis system and method designed by the present invention can realize one-time imaging of fluorescence resonance energy transfer by using spectrum or polarized beam splitter, and can simultaneously obtain the acceptor and donor emission spectra or detect the FRET efficiency Two digital pictures.
[0048] The four-dimensional visible microanalysis sys...
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Four-dimensional fluorescence resonance energy transfer efficiency visual microscopic analysis system and method, composed of microscopic three-dimensional coordinate automatic positioning and time-lapse tools, immobilized spectroscope and analysis imaging output module, to realize fluorescence resonance energy transfer donor fluorescence and acceptor fluorescence The unique one-time digital imaging can analyze and observe the four-dimensional digital changes of fluorescence resonance energy transfer efficiency in cells or tissues in real time, concisely and quickly. The spatial and temporal variation trend of energy transfer, analyze the two-dimensional, three-dimensional or four-dimensional microscopic digital changes related to the efficiency of fluorescence resonance energy transfer, and realize the detection mode of direct visualization of the digital analysis results of microscopic observation according to the principle of fluorescence resonance energy transfer.
Description
technical field [0001] The invention belongs to the field of microscopic image calculation and analysis, and in particular relates to a four-dimensional fluorescence resonance energy transfer efficiency visible microscopic analysis system and method. Background technique [0002] Fluorescence resonance energy transfer is an energy transfer phenomenon between two fluorescent molecules that are very close to each other. When the emission spectrum of the donor fluorescent molecule overlaps with the absorption spectrum of the acceptor fluorescent molecule, and the distance between the two molecules is within 10nm, a non-radioactive energy transfer occurs, that is, the FRET phenomenon, so that the fluorescence of the donor The intensity is much lower than when it exists alone (fluorescence quenching), while the fluorescence emitted by the acceptor is greatly enhanced (sensitized fluorescence). [0003] The efficiency of energy transfer is inversely proportional to the distance o...
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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N21/6486
Inventor 郭军吴辉文张晓龙陈婷婷张家瑞
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE