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Bougainvillea spectabilis willd detoxification tissue culture method

A tissue culture and bougainvillea technology, applied in the field of plant tissue culture, can solve problems such as affecting bougainvillea's expansion of reproduction and market share, limited propagation time, and small proliferation coefficient, and achieves overcoming technical barriers, quality improvement, and germplasm. The effect of resource preservation

Inactive Publication Date: 2018-09-14
山东亚龙生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous deepening of people's research on Bougainvillea and the rapid development of landscaping construction, the traditional propagation methods of Bougainvillea can no longer meet the needs of the market, because the traditional propagation methods have slow reproduction speed, small multiplication coefficient, and long rooting time. Long, limited breeding time and other disadvantages; and with the progress of bougainvillea breeding research, people have bred many new varieties with higher ornamental value, and some new varieties are difficult to reproduce with traditional breeding methods, and it is difficult Provide a large number of seedlings of excellent varieties or strains, thus affecting the expansion and market share of bougainvillea

Method used

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  • Bougainvillea spectabilis willd detoxification tissue culture method
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  • Bougainvillea spectabilis willd detoxification tissue culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1, a kind of Bougainvillea detoxification tissue culture method, comprises:

[0054] (1) Material selection: For pruning, select healthy and healthy Bougainvillea branches that are 1-2 years old and have no rot and no disease;

[0055] (2) Pregermination: insert the selected bougainvillea branches into a bottle with water, place them in a light room, maintain a temperature of 28~30°C, light intensity of 3000Lux, light time of 24h, irradiate for 7 days, and select 3~7cm bougainvillea buds Body segment;

[0056] (3) Surface treatment: Rinse the collected bougainvillea sprouts with running water, wash with 2% Tween-80 for 10-15min, and rinse with running water;

[0057] (4) Disinfection: Soak in 75% alcohol for 55 seconds, disinfect with 0.1% HgCl solution for 5 minutes, and rinse with sterile water;

[0058] (5) Cutting: renew the incision of the sterilized Bougainvillea bud body, and keep 2~3cm;

[0059] (6) Primary culture: inoculate the treated bougainvil...

Embodiment 2

[0064] Embodiment 2 A kind of Bougainvillea detoxification tissue culture method comprises:

[0065] (1) Material selection: For pruning, select healthy and healthy Bougainvillea branches that are 1-2 years old and have no rot and no disease;

[0066] (2) Pregermination: insert the selected bougainvillea branches into a bottle with water, place them in a light room, maintain a temperature of 26~28°C, light intensity of 5000Lux, light time of 24 hours, and irradiate for 5 days, select 3~7cm bougainvillea buds Body segment;

[0067] (3) Surface treatment: Rinse the collected bougainvillea sprouts with running water, wash with 2% Tween-80 for 10-15min, and rinse with running water;

[0068] (4) Disinfection: Soak in 75% alcohol for 55s, disinfect with 0.1% HgCl solution for 10min, rinse with sterile water;

[0069] (5) Cutting: renew the incision of the sterilized Bougainvillea bud body, and keep 2~3cm;

[0070] (6) Primary culture: inoculate the treated bougainvillea segments...

Embodiment 3

[0075] Embodiment 3 A kind of Bougainvillea detoxification tissue culture method, comprises:

[0076] (1) Material selection: For pruning, select healthy and healthy Bougainvillea branches that are 1-2 years old and have no rot and no disease;

[0077] (2) Pregermination: Insert the selected bougainvillea branches into a bottle with water, place them in a light room, maintain a temperature of 25~26°C, light intensity of 4000Lux, light time of 24h, irradiate for 10d, and select 3~7cm bougainvillea buds Body segment;

[0078] (3) Surface treatment: Rinse the collected bougainvillea sprouts with running water, wash with 2% Tween-80 for 10-15min, and rinse with running water;

[0079] (4) Disinfection: Soak in 75% alcohol for 55s, disinfect with 0.1% HgCl solution for 10min, rinse with sterile water;

[0080] (5) Cutting: renew the incision of the sterilized Bougainvillea bud body, and keep 2~3cm;

[0081] (6) Primary culture: inoculate the treated bougainvillea segments on med...

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Abstract

The invention discloses a bougainvillea spectabilis willd detoxification tissue culture method. The method comprises the following steps of (1) selecting materials, pruning, and selecting 1-2-year-old, full-bud, non-rot, non-disease and strong bougainvillea spectabilis willd branches; (2) germinating, and germinating the selected bougainvillea spectabilis willd branches; (3) treating the surfaces;(4) disinfecting; (5) cutting; (6) primarily culturing; (7) conducting proliferation and secondary culture, and inoculating sterile seedlings onto a corresponding medium for the proliferation and secondary culture; (8) conducting rooting culture; (9) domesticating. The method has the advantages that the quality of the bougainvillea spectabilis willd is greatly improved through bougainvillea spectabilis willd tissue culture, the cost is minimized, the coefficient of reproduction is improved, a technical basis is provided for bougainvillea spectabilis willd test-tube seedling industry production, important significance is achieved, the technical barriers in the cultivation process of bougainvillea spectabilis willd tissues are overcome, and good cultivation effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a bougainvillea virus-free tissue culture method. Background technique [0002] Bougainvillea is an evergreen woody vine plant belonging to the bougainvillea of ​​the family Mirabilis. It belongs to the flower-viewing shrubs in the flower-viewing trees. It is an important ornamental flower and an excellent landscaping plant. Its figure is graceful, its branches and leaves are green and soft, and it blooms for half a year from early autumn to late spring of the following year. As a novel and unique flower with extensive cultivation and management, it is deeply loved by people. When Bougainvillea is cultivated as a flower, it is often trimmed into shrubs, small trees or multi-layered pagodas, so it is called "Bougainvillea" in Japan; Red, white, orange, purple, multiple colors and other colors, colorful like Rhododendron, so it is called "Le Rhododendron" ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 金红梅李亚
Owner 山东亚龙生物科技有限公司
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