Applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation
A technology of tetrodotoxin and lung cancer cells, which is applied in the field of medical drug research and development, can solve the problems of low specificity of drug action, difficult targeted treatment, and large drug side effects, so as to enhance the ability of prevention and cure, and reduce migration and proliferation. ability, the effect of promoting the occurrence of apoptosis
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[0034] In the present invention, the method for preparing the puffer fish liver oil preferably includes the following steps: mixing the puffer fish liver with water, decoction for 2 to 2.5 hours, and collecting the upper layer of puffer fish liver oil. In the present invention, the decocting time is preferably 2.2h.
[0035] In the present invention, the mixing mass ratio of the puffer fish liver and water is (3-5):1, more preferably 4:1. In the present invention, the decoction temperature is 120°C to 130°C, more preferably 125°C. After decocting, the oil yield is about 75%, and the oil is yellow. After collecting the upper layer of puffer fish liver oil, the present invention preferably adopts a mesh screen to remove the oil residue and refrigerates. In the present invention, the size of the mesh screen is preferably 40 mesh. In the present invention, the temperature of the refrigeration is preferably 4°C.
[0036] The present invention also provides a preparation method of the...
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[0043] Example 1
[0044] Preparation of tetrodotoxin mother liquid: weigh 50 μg of tetrodotoxin with a purity of 99% and dissolve it in 1 mL of puffer fish liver oil to prepare a medicine mother liquid.
[0045] Put the puffer fish liver into the pot, add 25% water according to the weight of the liver, simmer for 2 hours on a direct fire at 120°C, the oil yield is about 75%, and the oil is dark yellow. After collection, it is removed with a 40-mesh sieve Put the oil residue in a refrigerator at 4°C for later use.
[0046] Prepare a medicinal solution containing 1μg / mL of tetrodotoxin. First prepare 98mL of basic carrier, weigh 85mL of puffer fish liver oil, then add 3mL of turmeric oil, 2mL of geraniol oil, 2mL of sweet almond oil, 2mL of wheat germ oil, 4mL of Brucea javanica oil, and use a heated magnetic stirrer to heat the basic carrier to 60 After temperature, add 2 mL of mother liquor containing tetrodotoxin, stir for 90 minutes at a constant temperature of 60°C at 100 rpm t...
Example Embodiment
[0047] Example 2
[0048] Preparation of tetrodotoxin mother liquid: weigh 50 μg of tetrodotoxin with a purity of 99% and dissolve it in 1 mL of puffer fish liver oil to prepare a medicine mother liquid.
[0049] Put the puffer fish liver into the pot, add 25% water according to the weight of the liver, simmer for 2 hours on a direct fire at 120°C, the oil yield is about 75%, and the oil is dark yellow. After collection, it is removed with a 40-mesh sieve Put the oil residue in a refrigerator at 4°C for later use.
[0050] Prepare a medicinal solution containing tetrodotoxin 0.5μg / mL. First prepare 99mL basic carrier, weigh 85mL of puffer fish liver oil, then add 2mL turmeric oil, 2mL geraniol oil, 3mL sweet almond oil, 3mL wheat germ oil, 4mL Brucea javanica oil, and heat the basic carrier to 60 with a heating magnetic stirrer. After temperature, add 1mL of mother liquor containing tetrodotoxin, stir for 90 minutes at a constant temperature of 60°C at 100 rpm to make it fully diss...
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