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Applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation

A technology of tetrodotoxin and lung cancer cells, which is applied in the field of medical drug research and development, can solve the problems of low specificity of drug action, difficult targeted treatment, and large drug side effects, so as to enhance the ability of prevention and cure, and reduce migration and proliferation. ability, the effect of promoting the occurrence of apoptosis

Inactive Publication Date: 2018-09-14
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese herbal medicines in China are widely available and relatively low in price. However, the ingredients of the medicines are complex and the specificity of the medicine effects is not strong. It is difficult to use them for targeted treatment and can only be used for postoperative recovery.
Although chemically synthesized drugs are more targeted, they have relatively large side effects, have a greater impact on normal cells, the patient's body, and diet, and are more resistant to tolerance. At the same time, they are expensive and difficult for ordinary patients long-term use

Method used

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  • Applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation
  • Applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation
  • Applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation

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preparation example Construction

[0034] In the present invention, the preparation method of puffer fish liver oil preferably includes the following steps: mixing puffer fish liver with water, decocting for 2-2.5 hours, and collecting the upper layer of puffer fish liver oil. In the present invention, the decoction time is preferably 2.2h.

[0035] In the present invention, the mixed mass ratio of puffer fish liver and water is (3-5):1, more preferably 4:1. In the present invention, the decocting temperature is 120°C-130°C, more preferably 125°C. After decocting, the oil yield is about 75%, and the oil quality is yellow. After collecting the upper layer puffer liver oil, the present invention preferably adopts a mesh screen to remove the oil residue and then refrigerates. In the present invention, the size of the mesh screen is preferably 40 mesh. In the present invention, the temperature of the cold storage is preferably 4°C.

[0036] The present invention also provides a preparation method of the medicine...

Embodiment 1

[0044] Preparation of tetrodotoxin mother solution: 50 μg of tetrodotoxin with a purity of 99% was weighed and dissolved in 1 mL of tetrodotoxin liver oil to prepare a drug mother solution.

[0045] Put the liver of puffer fish into the pot, add 25% water according to the weight of the liver, and fry it with direct fire at 120°C for 2 hours, the oil yield is about 75%, and the oil is dark yellow. After collection, remove it with a 40-mesh sieve Put the oily residue in a refrigerator at 4°C and refrigerate for later use.

[0046] Prepare a drug solution containing tetrodotoxin 1 μg / mL. First prepare 98mL of basic carrier, weigh 85mL of puffer fish liver oil, then add zedoary oil 3mL, geranium oil 2mL, sweet almond oil 2mL, wheat germ oil 2mL, javanica javanica oil 4mL, use a heating magnetic stirrer to heat the basic carrier to 60 After ℃, add 2 mL of tetrodotoxin-containing mother solution, stir at 100 rpm at 60 ℃ for 90 minutes to fully dissolve, cool at room temperature and...

Embodiment 2

[0048] Preparation of tetrodotoxin mother solution: 50 μg of tetrodotoxin with a purity of 99% was weighed and dissolved in 1 mL of tetrodotoxin liver oil to prepare a drug mother solution.

[0049] Put the liver of puffer fish into the pot, add 25% water according to the weight of the liver, and fry it with direct fire at 120°C for 2 hours, the oil yield is about 75%, and the oil is dark yellow. After collection, remove it with a 40-mesh sieve Put the oily residue in a refrigerator at 4°C and refrigerate for later use.

[0050] Prepare a drug solution containing tetrodotoxin 0.5 μg / mL. First, prepare 99 mL of basic carrier, weigh 85 mL of puffer fish liver oil, then add 2 mL of zedoary oil, 2 mL of geranium oil, 3 mL of sweet almond oil, 3 mL of wheat germ oil, and 4 mL of javanica javanica oil, and heat the basic carrier to 60 After ℃, add 1 mL of tetrodotoxin-containing mother solution, stir at 60 ℃ and 100 rpm for 90 minutes to fully dissolve, cool at room temperature and...

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Abstract

The invention relates to applications of tetrodotoxin in preparation of drugs used for inhibiting lung cancer cell migration and propagation, and belongs to the technical field of medicine science medicine development. The drugs capable of inhibiting lung cancer cell migration and propagation comprise 0.5 to 2<mu>g / ml of tetrodotoxin and a drug carrier, and are capable of reducing lung cancer cellmigration and propagation capacity effectively, and promoting cell apoptosis.

Description

technical field [0001] The invention relates to the technical field of research and development of medical drugs, in particular to the application of tetrodotoxin in the preparation of drugs for inhibiting lung cancer cell migration and proliferation. Background technique [0002] Currently in China, cancer has become the number one cause of death, and the morbidity and mortality are still rising, posing a huge threat to public health. In 2016, more than 2.8 million people died of cancer in China, an average of 7,500 people per day. About 570,000 patients died of lung cancer, and the survival rate of lung cancer patients was only 16.1%. Therefore, lung cancer is known as "the world's number one cancer killer" with its high lethality. Among them, the invasion and metastasis of lung cancer as a highly metastatic malignant tumor to distant tissues and organs is an important cause of death of cancer patients. Therefore, the ability to control invasion and metastasis has becom...

Claims

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Application Information

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IPC IPC(8): A61K31/529A61P35/00A61P35/04
CPCA61K31/529A61P35/00A61P35/04
Inventor 徐跑赵继红蒋火金强俊李红霞赵琦
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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