Preparation method of fish scale collagen

A technology of fish scale collagen and collagen powder, which is applied in the field of biological collagen preparation, can solve the problems of little processing and utilization of fish scales and other wastes, and achieves the effect of high hydrolysis efficiency

Inactive Publication Date: 2018-09-14
贝尔特(烟台)海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many freshwater fish processing processes are only limited to the utilization of fish body muscles, while the processing and utilization of wastes such as fish scales are seldom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A preparation method of fish scale collagen, comprising the steps of:

[0025] Step 1, put the fish scales into the water tank, add warm water 10 times the weight of the fish scales, and soak for 1 hour at a water temperature of 40°C to rehydrate the fish scales;

[0026] Step 2, soaking the treated fish scales in 0.5% hydrochloric acid 10 times the weight of the fish scales for 2 hours at 40°C;

[0027] Step 3, using 0.2% sodium hydroxide to neutralize the solution soaked with fish scales obtained in step 2 to neutrality;

[0028] Step 4, washing the fish scales after step 3 until neutral;

[0029] Step 5, adding desalted water 10 times the weight of fish scales to the fish scales, heating and boiling for 0.5 hours;

[0030] Step 6, filter, remove fish scales, and cool the temperature to 45°C;

[0031] Step 7, adding 0.1% mass protease to the cooled solution and incubating for 5 hours;

[0032] Step 8, using a diatomaceous earth filter to filter the hydrolyzed coll...

Embodiment 2

[0037] A preparation method of fish scale collagen, comprising the steps of:

[0038] Step 1, put the fish scales into the water tank, add warm water 10 times the weight of the fish scales, and soak for 1 hour at a water temperature of 40°C to rehydrate the fish scales;

[0039] Step 2, soaking the treated fish scales in 0.5% hydrochloric acid 10 times the weight of the fish scales for 2 hours at 40°C;

[0040] Step 3, using 0.2% sodium hydroxide to neutralize the solution soaked with fish scales obtained in step 2 to neutrality;

[0041] Step 4, washing the fish scales after step 3 until neutral;

[0042] Step 5, adding desalted water 10 times the weight of fish scales to the fish scales, heating and boiling for 0.5 hours;

[0043] Step 6, filter, remove fish scales, and cool the temperature to 45°C;

[0044] Step 7, add 0.1% mass protease to the cooled solution, and keep warm for 5 hours; the protease is a composition of trypsin and papain, and the ratio of the two is 1:1...

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PUM

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Abstract

The invention relates to a preparation method of fish scale collagen. The preparation method takes fish scales as raw materials and the fish scales are treated through steps of removing calcium, dissolving colloid, carrying out solid-liquid separation and filtering, hydrolyzing, carrying out collagen filtering and separation, carrying out macromolecular protein filtering and separation, desalting,dehydrating, drying and the like to prepare collagen powder; the fish scales are changed into valuable things and sufficient utilization of resources is realized. According to the preparation method,macromolecular protein is hydrolyzed into small molecular protein by adopting a combined biological enzymolysis method; biological enzyme has high enzymolysis efficiency, is safe and has no toxicity;the prepared collagen can meet food safety requirements.

Description

technical field [0001] The invention relates to the technical field of preparation of biological collagen, in particular to a preparation method of fish scale collagen. Background technique [0002] In recent years, the freshwater fish processing industry has developed rapidly with the growth of freshwater fish production in my country, and fish processing has become more and more extensive. However, many freshwater fish processing processes are only limited to the utilization of fish body muscles, while the processing and utilization of wastes such as fish scales are seldom. The total amount of waste in the freshwater fish processing industry reaches more than 2 million tons every year, of which fish scales account for about 15%, or 300,000 tons. If it can be used rationally, it can effectively reduce environmental pollution and improve the efficiency of enterprises. Therefore, increasing the utilization of substances contained in fish scales can be applied to many industr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/78C07K1/34C07K1/14C12P21/06
CPCC07K14/78C12P21/06
Inventor 惠成岳周述慧
Owner 贝尔特(烟台)海洋生物科技有限公司
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