Novel mercury-free environmentally-friendly hematoxylin dyeing liquid and preparation method thereof

A technology of hematoxylin and dye solution, which is applied in the field of pathology experiments, and can solve the problems of foam formation in dye solution, unclear nuclei and cytochrome staining levels, precipitation, etc.

Active Publication Date: 2018-09-14
HARBIN GREEN SPECIMEN TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that the existing hematoxylin staining solution is easy to produce precipitation, the color of nuclear staining is dark and the coloring layer of nuclei and cytoplasm is not clear, and the staining solution forms foam.

Method used

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  • Novel mercury-free environmentally-friendly hematoxylin dyeing liquid and preparation method thereof
  • Novel mercury-free environmentally-friendly hematoxylin dyeing liquid and preparation method thereof
  • Novel mercury-free environmentally-friendly hematoxylin dyeing liquid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1: Disodium ethylenediaminetetraacetic acid (EDTA) addition amount

[0048] This embodiment provides a hematoxylin dye solution, which is mainly prepared from the following components: hematoxylin, potassium aluminum sulfate dodecahydrate, sodium iodate, acetic acid, disodium edetate, iso Structure tridecyl polyoxyethylene ether, glycerin and water, wherein: the mass ratio of potassium aluminum sulfate dodecahydrate to hematoxylin is 24:4 (g / g); the mass ratio of sodium iodate to hematoxylin is 0.4:4 (g / g); the ratio of acetic acid to hematoxylin is 10:4 (mL / g); the ratio of glycerol to hematoxylin is 120:4 (mL / g), and the ratio of isomerized tridecanyl polyoxyethylene ether to hematoxylin is 5: 4 (mL / g); Adjust the addition of disodium edetate, make the ratio of disodium edetate and hematoxylin mass be respectively 0.1:4 (g / g) (experimental group 1), 0.2: 4(g / g) (experimental group 2), 0.3:4(g / g) (experimental group 3), 0.4:4(g / g) (experimental group 4), 0...

Embodiment 2

[0060] Embodiment 2: isomeric tridecanol polyoxyethylene ether addition amount

[0061] This embodiment provides a hematoxylin dye solution, which is mainly prepared from the following components: hematoxylin, potassium aluminum sulfate dodecahydrate, sodium iodate, acetic acid, disodium edetate, iso Structure tridecyl polyoxyethylene ether, glycerin and water, wherein: the mass ratio of potassium aluminum sulfate dodecahydrate to hematoxylin is 24:4 (g / g); the mass ratio of sodium iodate to hematoxylin is 0.4:4 (g / g); the ratio of acetic acid to hematoxylin is 10:4 (mL / g); the ratio of glycerol to hematoxylin is 120:4 (mL / g); the ratio of disodium edetate to hematoxylin is 0.4:4 (g / g); Adjust the addition amount of isomeric tridecyl alcohol polyoxyethylene ether, make the ratio (mL / g) of isomeric tridecyl alcohol polyoxyethylene ether and hematoxylin be respectively 2:4 (experimental group 1), 3:4 (experimental group 2), 4:4 (experimental group 3), 5:4 (experimental group 4...

Embodiment 3

[0069] Embodiment 3: the determination of mordant test addition amount

[0070] This embodiment provides a hematoxylin dye solution, which is mainly prepared from the following components: hematoxylin, mordant reagent, sodium iodate, acetic acid, disodium ethylenediaminetetraacetic acid, isotridecyl alcohol Polyoxyethylene ether, glycerin and water, wherein: the mass ratio of sodium iodate to hematoxylin is 0.4:4 (g / g); the ratio of acetic acid to hematoxylin is 10:4 (mL / g); the ratio of glycerol to hematoxylin is 120:4(mL / g), the mass ratio of disodium edetate to hematoxylin is 0.4:4(g / g), the ratio of isomeric tridecyl alcohol polyoxyethylene ether to hematoxylin is 5:4(mL / g); the rest is water. The mordant reagent is any one of potassium aluminum sulfate dodecahydrate, aluminum sulfate, ferric chloride and copper sulfate.

[0071] When the mordant reagent is potassium aluminum sulfate dodecahydrate, adjust the amount of potassium aluminum sulfate dodecahydrate so that th...

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Abstract

The invention discloses novel mercury-free environmentally-friendly hematoxylin dyeing liquid and a preparation method thereof and belongs to the technical field of a pathological experiment. The dyeing liquid is mainly prepared by the following raw materials: hematoxylin, a mordant reagent, an oxidizing agent, acid, ethylene diamine tetraacetic acid disodium salt, iso-tridecanol polyoxyethylene ether, glycerin and water; a Mordant is any one of aluminum potassium sulfate dodecahydrate, aluminum sulfate, ferric trichloride and copper sulfate; the oxidizing agent is any one of sodium iodate andpotassium permanganate; the acid is any one of acetic acid, hydrochloric acid and citric acid. The novel mercury-free environmentally-friendly hematoxylin dyeing liquid disclosed by the invention successfully solves the problem of HE dyeing troubled in the medical pathological diagnosis field for a long term, for example, hematoxylin dyeing liquid is poor in stability and short in shelf life, isprovided with a metal film, has dye residues, foams and is unclear in corpuscular contrast, unclear in level and dark in nuclear dyeing color and the like.

Description

technical field [0001] The invention relates to a novel mercury-free environment-friendly hematoxylin dye solution and a preparation method thereof, belonging to the technical field of pathological experiments. Background technique [0002] Hematoxylin stain is a routine nuclear stain in pathology laboratories, and its formulations are also varied. The main differences between the common hematoxylin dyes on the market are: 1. The oxidants are different. There are long-term natural oxidation, rapid oxidation using mercury oxide and potassium permanganate oxidant, and slow oxidation using sodium iodate oxidant; Second, the difference of mordant. Common mordants are aluminum sulfate, ferric chloride, potassium aluminum sulfate dodecahydrate, copper sulfate, etc.; 3. The acids used are different. Commonly used acids are acetic acid, hydrochloric acid, citric acid, etc. [0003] But in general, each formula of hematoxylin dye solution has common problems: 1. Excessive oxidatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N1/30
CPCG01N1/30G01N33/5005G01N2001/302
Inventor 夏东辉王金涛
Owner HARBIN GREEN SPECIMEN TECH DEV
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