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Application of CIITA gene as biomarker for diagnosis of dengue and dengue hemorrhagic fever

A technology for dengue hemorrhagic fever and dengue fever, which is applied in biochemical equipment and methods, microbial determination/examination, resistance to vector-borne diseases, etc. The effect of large amount, good detection sensitivity and specificity, and accurate results

Inactive Publication Date: 2018-09-14
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The clinical manifestations of dengue virus infection are very complex, and most of the symptoms are indistinguishable from diseases such as malaria, typhoid fever, and chikungunya fever. It is difficult to make a clear diagnosis based solely on the epidemiological data and clinical manifestations of patients, so laboratory tests are required for further diagnosis

Method used

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  • Application of CIITA gene as biomarker for diagnosis of dengue and dengue hemorrhagic fever
  • Application of CIITA gene as biomarker for diagnosis of dengue and dengue hemorrhagic fever
  • Application of CIITA gene as biomarker for diagnosis of dengue and dengue hemorrhagic fever

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Screening of genes differentially expressed in patients with dengue fever and / or dengue hemorrhagic fever and normal people

[0046] 1. Research object:

[0047] Disease group: Collect 5 patients with dengue fever, 5 patients with dengue hemorrhagic fever, all patients according to "WHO / TDR.Dengue-Guidelines for diagnosis, Treatment, prevention and control.2009; http: / / www.who.int / tdr / publications / training-guideline-publications / dengue-diagnosis-treatment / en / index.html.Date of Access: 12 / 05 / 2015.2009" for diagnosis.

[0048] Normal group: 4 healthy volunteers were selected.

[0049] There was no significant difference in age and gender between the two groups (P>0.10), which were comparable.

[0050] All research subjects were informed about this study and signed the informed consent.

[0051] 2. Extraction of total RNA in blood

[0052] (1) Fresh whole blood, erythrocyte lysate (1:1), mix by inverting several times, let stand for 5 minutes. 10000g, 4°C, ...

Embodiment 2

[0069] Example 2 Reverse transcription PCR experiment verifies the genes differentially expressed in patients with dengue fever and / or dengue hemorrhagic fever and normal people

[0070] The differentially expressed gene-CIITA gene screened out in Example 1 was selected for large sample verification.

[0071] 1. Research object:

[0072] Disease group: Collect 30 patients with dengue fever, 25 patients with dengue hemorrhagic fever, all patients according to "WHO / TDR.Dengue-Guidelines for diagnosis, Treatment, prevention and control.2009; http: / / www.who.int / tdr / publications / training-guideline-publications / dengue-diagnosis-treatment / en / index.html.Date of Access: 12 / 05 / 2015.2009" for diagnosis.

[0073] Normal group: select 30 cases of healthy volunteers.

[0074] There was no significant difference in age and gender between the two groups (P>0.10), which were comparable.

[0075] All research subjects were informed about this study and signed the informed consent.

[0076] 2...

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Abstract

The invention discloses application of CIITA gene as a biomarker for diagnosis of dengue and dengue hemorrhagic fever. High-throughput screen is performed on genes having expression differences in normal people and patients with dengue and dengue hemorrhagic fever; large-sample reverse-transcription PCR (polymerase chain reaction) test proves that the content of CIITA gene in the blood of the patients with dengue and dengue hemorrhagic fever is lower than that in normal people. Therefore, the CIITA gene can serve as a biomarker for the diagnosis of dengue and dengue hemorrhagic fever. Researchresults herein provide a noninvasive method for the clinical early diagnosis of dengue and dengue hemorrhagic fever; the method is applicable to clinical popularization.

Description

technical field [0001] The invention belongs to the field of epidemiological diagnosis and prevention, and relates to a gene diagnostic marker for dengue fever and dengue hemorrhagic fever. Background technique [0002] Since the Antonine plague (Shrap R, Lotka A J.A Problem in AgeDistribution[J].Philosophical Magazine, 1911,21:35-438) in the 2nd century AD, infectious diseases have been endangering human health and brought huge impacts on social stability. Impact. In recent years, the incidence of dengue infectious disease has increased rapidly and has become a world public health problem, and it is recorded as a disease in Appendix II of the newly revised International Health Regulations (2005). Dengue fever is an acute infectious disease caused by dengue virus and transmitted by Aedes mosquitoes. At first, people named it joint fever and fracture fever according to its symptoms (Monath, T.P. Dengue: The risk to developed and developing countries: Proc. Nat. Acad. Sci. U...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/6869C12Q1/686C12Q1/6851C12Q1/6841C12Q1/6837G01N33/569
CPCC12Q1/6837C12Q1/6841C12Q1/6851C12Q1/686C12Q1/6869C12Q1/70G01N33/56983C12Q2535/122Y02A50/30
Inventor 贾宏毅向常娟
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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