Preparation method and application of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres
An immune sensor and molybdenum disulfide technology, which is applied in the field of nanomaterials and electrochemical analysis, can solve the problems of weak dispersion and conductivity, and achieve the effects of short response time, good catalytic effect and improved sensitivity
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Embodiment 1
[0033] Embodiment 1 A preparation method of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, the steps are as follows:
[0034] (1) Preparation of palladium-doped molybdenum disulfide nanospheres
[0035] 1) Add 0.5mmol ammonium molybdate tetrahydrate, 10mmol thiourea and 0.005mmol polyvinylpyrrolidone to 35 mL ultrapure water in sequence, and stir magnetically to form a homogeneous solution A;
[0036] 2) Transfer solution A to a high-temperature reaction kettle, react at 220ºC for 18 hours, centrifuge, wash with ultrapure water and absolute ethanol to remove impurities, and freeze-dry the product in a freeze dryer to obtain Molybdenum disulfide nanospheres;
[0037]3) Dissolve 30 mmol of hexadecyltrimethylammonium bromide in 10 mL of ultrapure water, and sonicate for 10 min to obtain a uniform solution B; then add 1 mg of molybdenum disulfide nanospheres to solution B, and the mixed solution becomes Turn into gray black, continue u...
Embodiment 2
[0045] Embodiment 2 A preparation method of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, the steps are as follows:
[0046] (1) Preparation of palladium-doped molybdenum disulfide nanospheres
[0047] 1) Add 1.0 mmol of ammonium molybdate tetrahydrate, 15 mmol of thiourea and 0.010 mmol of polyvinylpyrrolidone to 35 mL of ultrapure water in sequence, and magnetically stir to form a homogeneous solution A;
[0048] 2) Transfer solution A to a high-temperature reactor, react at 220ºC for 18 hours, centrifuge, wash with ultrapure water and absolute ethanol to remove impurities, and freeze-dry the product in a freeze dryer to obtain Molybdenum disulfide nanospheres;
[0049] 3) Dissolve 50 mmol of cetyltrimethylammonium bromide in 10 mL of ultrapure water and sonicate for 15 min to obtain a uniform solution B; then add 3 mg of molybdenum disulfide nanospheres to solution B, and mix the solution Turn into gray-black, continue ultrason...
Embodiment 3
[0057] Embodiment 3 A preparation method of an electrochemical immunosensor based on palladium-doped molybdenum disulfide nanospheres, the steps are as follows:
[0058] (1) Preparation of palladium-doped molybdenum disulfide nanospheres
[0059] 1) Add 1.5 mmol of ammonium molybdate tetrahydrate, 20 mmol of thiourea and 0.015 mmol of polyvinylpyrrolidone to 35 mL of ultrapure water in sequence, and stir magnetically to form a homogeneous solution A;
[0060] 2) Transfer solution A to a high-temperature reactor, react at 220ºC for 18 hours, centrifuge, wash with ultrapure water and absolute ethanol to remove impurities, and freeze-dry the product in a freeze dryer to obtain Molybdenum disulfide nanospheres;
[0061] 3) Dissolve 70 mmol of cetyltrimethylammonium bromide in 10 mL of ultrapure water and sonicate for 20 min to obtain a uniform solution B; then add 5 mg of molybdenum disulfide nanospheres to solution B, and mix the solution Turn into gray-black, continue ultrason...
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