A preparation method of microbial bacterial agent and fertilizer prepared by using microbial bacterial agent
A technology of microbial inoculum and fertilizer, which is applied to the field of fertilizer prepared by microbial inoculum and the preparation of microbial inoculum, can solve problems such as adverse effects on plant growth, and achieve the effect of increasing the effect of soil microorganisms
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[0019] The application provides a preparation method of microbial inoculum, comprising:
[0020] (1) Take 1 g of rhizosphere soil, add it to 9 ml of water, and oscillate ultrasonically for 10 to 20 minutes; take 1 ml of the oscillating solution for 10-fold gradient dilution, and take 5 to 10 ml of it and spread it on LB medium for cultivation to obtain a purified bacterial strain; The ingredients of the LB medium include: peptone 9.0-11.0g, yeast powder 4.0-6.0g, NaCl 9.0-11.0g, add distilled water to 1000ml, pH 7.0-7.2;
[0021] (2) Place the purified bacterial strain obtained in step (1) in the PVK medium, and screen out the Roseomonas sp.CH-Si-5 strain; the components of the PVK medium include: glucose 9.0~11.0g, NaCl 0.1~ 0.3g, MgSO 4 ·7H 2 O0.09~0.15g, KCl0.1~0.3g, (NH 4 ) 2 SO 4 0.4~0.6g, Ca 3 (PO 4 ) 2 4.0~6.0g, yeast extract 0.4~0.6g, MnSO 4 ·H 2 O 0.001~0.003g, FeSO 4 ·7H 2 O 0.001~0.003g, bromophenol blue 0.3~0.5g, pH 6.5~7.0, add distilled water to 1000...
Embodiment 1
[0031] A preparation method of microbial bacterial agent, comprising:
[0032] (1) Get 1 g of rhizosphere soil, add it to 9 ml of water, and ultrasonically vibrate for 15 min; get 1 ml of the oscillating solution for 10-fold gradient dilution, get 10 ml and apply it on LB medium to cultivate a purified bacterial strain; the LB medium The ingredients include: peptone 10.0g, yeast powder 5.0g, NaCl 10.0g, add distilled water to 1000ml, pH 7.2;
[0033](2) Place the purified bacterial strain obtained in step (1) in the PVK medium, and screen out the Roseomonas sp.CH-Si-5 strain; the components of the PVK medium include: glucose 9.0~11.0g, NaCl 0.1~ 0.3g, MgSO 4 ·7H 2 O0.09~0.15g, KCl 0.1~0.3g, (NH 4 ) 2 SO 4 0.4~0.6g, Ca 3 (PO 4 ) 2 4.0~6.0g, yeast extract 0.4~0.6g, MnSO 4 ·H 2 O 0.001~0.003g, FeSO 4 ·7H 2 O 0.001~0.003g, bromophenol blue 0.3~0.5g, pH 6.5~7.0, add distilled water to 1000ml;
[0034] Specifically, the Roseomonas sp.CH-Si-5 strain isolated in step (2...
Embodiment 2
[0043] A preparation method of microbial bacterial agent, comprising:
[0044] (1) Get 1 g of rhizosphere soil, add it to 9 ml of water, and ultrasonically vibrate for 15 min; get 1 ml of the oscillating solution for 10-fold gradient dilution, get 10 ml and apply it on LB medium to cultivate a purified bacterial strain; the LB medium The ingredients include: peptone 10.0g, yeast powder 5.0g, NaCl 10.0g, add distilled water to 1000ml, pH 7.2;
[0045] (2) Place the purified bacterial strain obtained in step (1) in the PVK medium, and screen out the Roseomonas sp.CH-Si-5 strain; the components of the PVK medium include: glucose 9.0~11.0g, NaCl 0.1~ 0.3g, MgSO 4 ·7H 2 O0.09~0.15g, KCl 0.1~0.3g, (NH 4 ) 2 SO 4 0.4~0.6g, Ca 3 (PO 4 ) 2 4.0~6.0g, yeast extract 0.4~0.6g, MnSO 4 ·H 2 O 0.001~0.003g, FeSO 4 ·7H 2 O 0.001~0.003g, bromophenol blue 0.3~0.5g, pH 6.5~7.0, add distilled water to 1ml;
[0046] Specifically, the Roseomonas sp.CH-Si-5 strain isolated in step (2) ...
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