Vaccination method for peanut stripe virus

A striped virus, peanut technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as multi-infectious virus materials, poor control of emery dosage, etc., to improve inoculation speed and inoculation efficiency, fast and efficient. The effect of identification and acceleration of disease resistance breeding and virus pathogenic mechanism research

Inactive Publication Date: 2018-09-28
SHANDONG PEANUT RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, mechanical friction inoculation requires more susceptible virus materials, and the amount of emery is not easy to control

Method used

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  • Vaccination method for peanut stripe virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Collect fresh peanut leaves infected by peanut streak virus with a hole puncher to obtain round virus leaves with a diameter of 0.9 cm, put them in a 1.5 mL centrifuge tube, and store at -70 °C;

[0029] Sow peanuts and inoculate after two pinnate compound leaves grow;

[0030] Take 0.02g of infected peanut leaves, add 800 μL of phosphate buffer solution with a pH of 7.2, 0.01g of corundum in turn, and use a 7.5cm grinding rod to grind the infected peanut leaves until they are homogenized. Wherein, the addition ratio of virus leaf and phosphate buffer is 1g virus leaf adds 40mL phosphate buffer, and the addition ratio of virus leaf and carborundum is 2:1;

[0031] Take 50 μL of the homogenate and place it on the peanut leaves to be inoculated, apply it gently 3 times in one direction, and spray it with water to rinse.

Embodiment 2

[0033] The inoculation method of peanut stripe virus is the same as that in Example 1, except that the pH value of the phosphate buffer solution is 7.3, and the addition ratio of virus leaves to carborundum is 1:1.

Embodiment 3

[0035] The inoculation method of peanut stripe virus is the same as that of Example 1, except that the pH value of the phosphate buffer solution is 7.4, and the addition ratio of virus leaves to carborundum is 3:1.

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Abstract

The invention provides a vaccination method for a peanut stripe virus and belongs to the technical field of plant virus vaccination. By means of the method, vaccination of the peanut stripe virus canbe simply and efficiently finished. According to the technical scheme, the step of spraying carborundum on a plant before vaccination is omitted, and the carborundum together with virus leaves and phosphate buffer is prepared into homogenate, so that efficient vaccination can be finished with small amount of virus leaves. The method has the advantages that vaccination speed and vaccination efficiency of the peanut stripe virus are improved, the resistance of peanuts to the stripe virus is favorably identified quickly and efficiently, and disease-resisting breeding selection and viral pathogenesis research are accelerated.

Description

technical field [0001] The invention belongs to the technical field of plant virus inoculation, and in particular relates to a peanut stripe virus inoculation method. Background technique [0002] At present, peanut streak virus occurs widely in the peanut production areas north of the Yangtze River in my country. Among virus diseases, peanut streak virus has the most serious impact on my country's peanut industry, and it is an important prevention and control target for peanut production. Efficient inoculation of peanut stripe virus is an important basis for the breeding of peanut stripe virus germplasm and the study of disease resistance mechanism, and is the key to the efficient prevention and control of peanut stripe virus. [0003] Mechanical friction inoculation is the most commonly used inoculation method for peanut streak virus at present. This method is generally completed by spraying emery on the peanut leaves when inoculating. However, mechanical friction inocula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/24
CPCC12Q1/24
Inventor 许曼琳迟玉成张霞董炜博吴菊香
Owner SHANDONG PEANUT RES INST
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