Microparticle forward scattered light detection device and detection method thereof

[0031] Example

[0032] This embodiment will be described with reference to the drawings. Such as Figure 1-5 As shown, a microparticle forward scattered light detection and detection device includes a platform 1, a microfluidic chip 3, a driving component 6, a light excitation component 2, a light detection component 4, and a data processing component 5. The platform 1 is a dark room Structure, the light excitation component 2 is embedded on the top of the platform 1, the microfluidic chip 3 and the light detection component 4 are fixed in the platform 1, the light detection component 4 is located under the microfluidic chip 3, and the driving component 6 passes through the platform 1 and the microfluidic control The chip 3 is connected, the light detection component 4 is connected to the data processing component 5, and the PC display terminal 21 is connected to the data processing component 5;

[0033] The microfluidic chip 3 is composed of a glass sheet 7 and a polydimethylsiloxane 8 fixed on the glass sheet. The surface of the polydimethylsiloxane 8 is provided with a sample reservoir 9 and a sheath liquid reservoir. The tank 10 and the waste liquid storage tank 11, the sample storage tank 9 is provided with a sample channel 13 communicating with the sample storage tank 9, and the sheath liquid storage tank 10 is provided with a sheath liquid channel 12 communicating with the sheath liquid storage tank 10. , The waste liquid storage tank 11 is provided with a detection channel 15 communicating with the waste liquid storage tank 11, the sample channel 13 and the sheath liquid channel 12 are vertically intersected and then communicated with the detection channel 15. The detection channel 15 is provided with a detection area 14, and the sample storage The liquid tank 9, the sheath liquid storage tank 10 and the waste liquid storage tank 11 are driven by the electric drive assembly 6;

[0034] The light excitation assembly 2 is composed of an LED 16, a focusing light path 18 and a filter I17, and a focusing light path 18 is provided between the LED 16 and the filter I17;

[0035] The light detection assembly 4 is composed of a filter II 19 and a photosensitive detector 20, and a photosensitive detector 20 is provided under the filter II 19.

[0036] The light emitted by the LED 16 is focused by the focusing light path 18 and then transmitted through the filter I17, irradiating the microparticles in the detection area 14 vertically, causing the microparticles to produce forward scattered light at a small angle, so as to pass through the filter II19 and be photosensitive Detector 20 received.

[0037] The data processing component 5 includes a microprocessor.

[0038] The LED 16 and the filter I17 in the light excitation assembly 2 have the same color. At the same time, the color of the LED 16 and the filter I17 in the light excitation assembly 2 and the filter II19 in the light detection assembly 4 are not the same. The area covered by the light spot of the light excitation component 3 is all over the detection area 14 of the microfluidic chip 2.

[0039] A method for detecting forward scattered light of microparticles includes the following steps:

[0040] Add 10μL of sheath solution to sheath solution reservoir 10, add 1μL of microparticle-containing solution to sample reservoir 9, turn on the light excitation module 2, the light detection module 4, the data processing module 5, and the electric drive module 6. The sheath solution and the sample solution flow along the sheath solution channel 12 and the sample channel 13 through the detection channel 15 to the waste liquid reservoir 11. When the microparticles in the sample solution pass through the detection area 14, they will be excited by the light excitation component to emit forward small-angle scattered light This light intensity will be received by the photosensitive detector in the light detection component 4, and the light signal will be converted into a digital pulse signal by the data processing component 5, and finally displayed on the PC display terminal 21, which can pass the strength and weakness of the pulse signal Quantity, which reflects the size and quantity of microparticles.