Rooting culture medium for sea buckthorn tissue culture and tissue culture method

A technology of rooting medium and sea buckthorn group, which is applied in the direction of medium, horticultural methods, botanical equipment and methods, etc., can solve the problems of low root activity, low rooting rate, and small number of roots, and achieves optimized hormone regulation and optimized light. Effect

Active Publication Date: 2018-10-09
新疆阿勒泰地区林业工作管理站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But existing seabuckthorn tissue culture rooting medium has few rooti

Method used

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  • Rooting culture medium for sea buckthorn tissue culture and tissue culture method

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Embodiment 1

[0024] The rooting medium for seabuckthorn tissue culture of the present invention is based on 1 / 2MS medium, and NH is added therein 4 NO 3 240mg / L, MgSO 4 ·7H 2 O 32mg / L, NaH 2 PO 4 ·H 2 O 18mg / L, inositol 3mg / L, glycine 0.1mg / L, indole-3-acetic acid (IAA) 0.1mg / L, sucrose 30g / L, agar 6g / L.

[0025] The preparation method is as follows: mixing all components except agar, heating with slow fire and stirring constantly to dissolve them, adjusting the pH value to 6.8 with acid such as dilute hydrochloric acid, adding agar, and performing damp heat sterilization after stirring.

[0026] Seabuckthorn tissue culture method of the present invention is as follows:

[0027] A single plant of seabuckthorn bushy seedlings was excised and inoculated into rooting medium to induce rooting. The culture conditions are as follows: temperature: 25° C., light intensity: 2400 lx, and photoperiod: 16 h / d to obtain test-tube plantlets. When the roots grow to more than 3.5 cm, the test-tub...

Embodiment 2

[0029] The rooting medium for seabuckthorn tissue culture of the present invention is based on 1 / 2MS medium, and NH is added therein 4 NO 3 230mg / L, MgSO 4 ·7H 2 O 35mg / L, NaH 2 PO 4 ·H 2 O 15mg / L, inositol 4mg / L, glycine 0.1mg / L, indole-3-acetic acid (IAA) 0.1mg / L, sucrose 30g / L, agar 6g / L.

[0030] The preparation method is as follows: mixing all components except agar, heating with slow fire and stirring continuously to dissolve them, adjusting the pH value to 6.6 with acid such as dilute hydrochloric acid, adding agar, and performing damp heat sterilization after stirring.

[0031] Seabuckthorn tissue culture method of the present invention is as follows:

[0032] A single plant of seabuckthorn bushy seedlings was excised and inoculated into rooting medium to induce rooting. The culture conditions are as follows: temperature: 23° C., light intensity: 2600 lx, and photoperiod: 16 h / d to obtain test-tube plantlets. When the roots grow to more than 3.5 cm, the test-t...

Embodiment 3

[0034] The rooting medium for seabuckthorn tissue culture of the present invention is based on 1 / 2MS medium, and NH is added therein 4 NO 3 250mg / L, MgSO 4 ·7H 2 O 30mg / L, NaH 2 PO 4 ·H 2 O 20mg / L, inositol 2mg / L, glycine 0.2mg / L, indole-3-acetic acid (IAA) 0.1mg / L, sucrose 30g / L, agar 6g / L.

[0035] The preparation method is as follows: mix all components except agar, heat slowly and stir constantly to dissolve, adjust the pH value to 7.0 with acid such as dilute hydrochloric acid, add agar, and carry out moist heat sterilization after stirring.

[0036] Seabuckthorn tissue culture method of the present invention is as follows:

[0037]A single plant of seabuckthorn bushy seedlings was excised and inoculated into rooting medium to induce rooting. The culture conditions are as follows: temperature: 27° C., light intensity: 2200 lx, and photoperiod: 16 h / d to obtain test-tube plantlets. When the roots grow to more than 3.5 cm, the test-tube seedlings are moved into the...

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Abstract

The invention provides a rooting culture medium for sea buckthorn tissue culture. The rooting culture medium is characterized by using a 1/2MS culture medium as a basal culture medium into which 230-250mg/L of NH4NO3, 30-35mg/L of MgSO4.7H2O, 15-20mg/L of NaH2PO4.H2O, 2-4mg/L of inositol, 0.1-0.2mg/L of glycine, 0.1mg/L of indole-3-acetic acid, 30g/L of sucrose and 6g/L of agar are added. The invention provides the rooting culture medium for sea buckthorn tissue culture; on the basis of the 1/2MS culture medium, through adjustment of the types and the concentrations of major elements, optimization of hormone regulation, optimization of light irradiation and the like, the rooting culture medium with balanced and reasonable nutrients is provided for tissue-cultured sea buckthorn rooting seedlings, so that fast, efficient and robust growth of the sea buckthorn rooting seedlings can be promoted.

Description

technical field [0001] The invention belongs to the technical field of plant regeneration through tissue culture technology, and in particular relates to a rooting medium and a tissue culture method for seabuckthorn tissue culture. Background technique [0002] Seabuckthorn (Latin scientific name: Hippophae rhamnoides Linn.) is a perennial deciduous fruit tree, shrub or small tree. Its fruit, peel, leaf, bark and its processed products contain more than 280 kinds of physiologically active substances, which have strong special effects of anti-inflammatory, bactericidal, pain-relieving and promoting tissue regeneration. Many of the ingredients have shown miraculous therapeutic effects in killing and inhibiting tumor cells, anti-radiation, anti-coagulation, lowering blood pressure, preventing vascular embolism, anti-aging, anti-fatigue, enhancing body vitality and immunity. [0003] Seabuckthorn has luxuriant branches and leaves, well-developed lateral roots, and strong root t...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G31/00A01G24/15A01G24/28
CPCA01G24/15A01G24/28A01G31/00A01H4/001A01H4/008
Inventor 赵英徐航刘伟郑新国胡茵韩晓燕张志刚
Owner 新疆阿勒泰地区林业工作管理站
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