Medicine box for immunotherapy of malignant tumors and application thereof
A malignant tumor and immunotherapy technology, applied in the field of medicine, can solve the problems of unclear mechanism of action of traditional Chinese medicine, disorder of microbiota, no tumor treatment, etc., and achieve the effect of strengthening recognition and presentation ability, promoting maturation and differentiation, and avoiding functional inhibition
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[0036] Embodiment: pharmacological test
[0037] 1. Materials and methods
[0038] 1.1 Test animals
[0039] Animals: Balb / c mice, male, 6-8 weeks old, 18±2g.
[0040] 1.2 Model establishment
[0041] Take H22 mouse liver cancer cells in good growth state and logarithmic growth phase, digest the cells with 0.25% trypsin to make them into single cells, wash twice with PBS, adjust the cell concentration to 2 with normal saline by cell counting method ×10 7 cell / ml, each healthy Balb / c male mouse was subcutaneously inoculated with 0.1ml (containing 2×10 6 During the feeding process, the mice in each group were given the same diet, and the relative humidity in the animal room was kept at 20-30%.
[0042] 1.3 Grouping and administration
[0043] Control group: intragastric administration of normal saline, once a day, for 22 consecutive days;
[0044] Intervention group 1: The daily oral dose of LY2157299 is 1.5 mg, and the oral administration is continued for 22 days;
[00...
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