Lysinibacilus xylanilyticus LX024 for controlling wheat stem rot and head blight and application thereof

A technology of Bacillus lysinosa and Bacillus lysinum, which is applied to the biocontrol of Bacillus xylolysinica LX024 and its application fields, can solve the problems of secondary pollution, reduce residues and save costs Effect

Active Publication Date: 2018-10-19
HUAIYIN INSTITUTE OF TECHNOLOGY
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  • Summary
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  • Application Information

AI Technical Summary

Problems solved by technology

Although chemical control can effectively control the development of disease in the field, it is very easy to cause secondary pollution to the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Screening method: Bacillus xylosinolyticum LX024 (CGMCC No. 14645) was isolated from wheat rhizosphere soil in Wharf Town, Huaian City, Jiangsu Province. Cut the wheat roots into 1cm pieces, soak them in 1% sodium hypochlorite for 5 minutes, soak them in 70% ethanol for 1-2 minutes, and wash them with sterile water for 3 times (take 100 μl of the last cleaning solution and spread it on the R2A solid medium for cultivation. If there is aseptic growth after 48 h, the surface is considered to be clean and sterile). Take 3 g of sterilized samples and place them in a sterile mortar, add 27ml of sterile 0.85% NaCl, macerate the tissue, grind, and dilute with sterile 0.85% NaCl gradients. Take 100 μl each of the three gradient dilutions of 10 -1 , 10 -2 , and 10 -3 and spread them on R2A, and incubate at 28°C for 48 h. Pick the largest single colony and inoculate it into fresh R2A solid medium, and obtain purified strains through repeated transfer. The purified strains were ...

Embodiment 2

[0025] The preparation of embodiment 2 LX024 biocontrol agent

[0026] Inoculate the Bacillus xylosinolyticus LX024 (CGMCC No. 14645) strain into LB culture medium and shake it at 180rpm at 28°C for 16 hours, then take samples every 2 hours in the ultra-clean workbench to measure its OD at 600 nm value, when the OD value was 0.8, the culture was terminated, and this bacterial solution was used as the seed bacterial solution. The seed bacteria solution was inoculated into the LB fermentation medium at a volume ratio of 1:500 for fermentation, cultured at 28°C and 180 rpm for 48 hours, then centrifuged at 6000 rpm for 10 minutes, and the precipitate was collected to obtain the biocontrol agent LX024. Dilute the biocontrol agent LX024 with sterilized water, and the total concentration of viable bacteria in the obtained biocontrol agent is 1×10 8 ~ 1×10 9 CFU / mL.

Embodiment 3

[0027] Example 3 Control effect of biocontrol bacteria LX024 on wheat scab and stem rot in greenhouse and plot test

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Abstract

The invention discloses lysinibacilus xylanilyticus LX024 for controlling wheat stem rot and head blight and application thereof. The strain is lysinibacilus xylanilyticus preserved in the China General Microbiological Culture Collection Center on Sept. 19, 2017 with a preservation number of CGMCC No.14645. A biocontrol inoculant prepared through liquid fermentation culture of LX024 strain has a good effect on controlling wheat stem rot and head blight. The use of the biocontrol inoculant LX024 at seedling and growth stages of wheat has a certain control effect on wheat stem rot and head blight, and the control effect reaches 65.01% and 65.81% respectively.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a biocontrol xylosyllysinyl bacillus LX024 used for preventing and treating wheat stalk rot and scab and its application. Background technique [0002] Wheat scab and stalk rot are global diseases caused by Fusarium graminearum, which seriously affect the yield and quality of wheat. Among them, wheat stalk rot is a kind of complex infectious soil-borne disease that is invaded by a variety of pathogenic bacteria and occurs mixedly. It is also known as sickle root rot and sickle stalk rot. The disease is a worldwide important disease, and more than 10 countries have reported its occurrence. In my country, Jiangsu has reported the harm of wheat stem base brown rot. According to the investigation of the wheat soil-borne disease research group of the Plant Protection College of Henan Agricultural University, the disease generally occurs in the main wheat producing areas of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G7/06A01G13/00C12R1/01
CPCA01G7/06A01G13/00A01N63/00C12N1/205C12R2001/01
Inventor 徐海亮王云鹏王晓莉王纪忠王利国
Owner HUAIYIN INSTITUTE OF TECHNOLOGY
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