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Method for rapidly testing purity of nuciferine

A technology for rapid determination of lotus leaf alkaloid, applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of high measurement cost, high instrument requirements, and many steps for the determination of lotus leaf alkaloid purity by HPLC method, and achieves low measurement cost, The method is simple, and the effect of reducing the labor intensity and number of experiments

Active Publication Date: 2018-10-19
安徽粮食工程职业学院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the current situation that there are many steps for measuring the purity of nucifera, the HPLC method has high measurement costs and high requirements for instruments, the present invention proposes a method for quickly measuring the purity of nucifera, and the method for measuring the purity of nucifera comprises the following step:

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  • Method for rapidly testing purity of nuciferine
  • Method for rapidly testing purity of nuciferine
  • Method for rapidly testing purity of nuciferine

Examples

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Embodiment 1

[0030] The present embodiment provides a method for rapidly measuring the purity of nuciferine, and the specific operation method is as follows:

[0031] 1. Accurately weigh 50.00mg of nuciferine sample, dilute it to 10mL with first-grade pure water, and let it stand for 2~3h to obtain the mixed solution Ⅰ.

[0032] 2. After filtering the mixed solution I, wash with first-grade pure water 2 to 3 times to obtain the washed nuciferine sample, and dry it at 105° C. to obtain the washed nuciferine.

[0033] 3. Dissolve the washed nuciferine with 10mL of methanol, oscillate fully, and filter with a filter cloth with a pore size of 100 μm, take the filtrate, and remove the methanol solvent by rotating the filtrate at 40°C and 0.2MPa to obtain a methanol extract .

[0034] 4. Dissolve the methanol extract with 10mL ethyl acetate, shake fully, and filter with a filter cloth with a pore size of 100μm, take the filtrate, and remove the ethyl acetate solvent by rotary evaporation at 70°...

Embodiment 2

[0043] The present embodiment provides a method for rapidly measuring the purity of nuciferine, and the specific operation method is as follows:

[0044] 1. Accurately weigh 30.00mg of nuciferine sample, dilute to 10mL with first-grade pure water, and let it stand for 2~3h to obtain the mixed solution Ⅰ.

[0045] 2. After filtering the mixed solution I, wash it with first-grade pure water for 2~3 times to obtain the washed nuciferine sample, and place it at 105°C for drying for 10 minutes to obtain the washed nuciferine sample.

[0046] 3. Dissolve the washed nuciferine with 50 mL of methanol, shake fully, and filter with a filter cloth with a pore size of 500 μm, take the filtrate, and remove the methanol solvent by rotating the filtrate at 30 °C and 0.1 MPa to obtain a methanol extract .

[0047]4. Dissolve the methanol extract with 10mL ethyl acetate, shake fully, and filter with a filter cloth with a pore size of 500μm, take the filtrate, and remove the ethyl acetate solv...

Embodiment 3

[0053] The present embodiment provides a method for rapidly measuring the purity of nuciferine, and the specific operation method is as follows:

[0054] 1. Accurately weigh 100.00mg of nuciferine sample, dilute it to 25mL with first-grade pure water, and let it stand for 2~3h to obtain the mixed solution Ⅰ.

[0055] 2. After filtering the mixed liquid I, wash it with first-grade pure water for 2~3 times to obtain the washed nuciferine sample, and dry it at 105±5°C for 10 minutes to obtain the washed nuciferine .

[0056] 3. Dissolve the washed nuciferine with 100mL of methanol, shake fully, and filter with a filter cloth with a pore size of 50 μm, take the filtrate, and remove the methanol solvent by rotating the filtrate at 60°C and 0.6MPa to obtain a methanol extract .

[0057] 4. Dissolve the methanol extract with 10mL ethyl acetate, shake fully, and filter with a filter cloth with a pore size of 500μm, take the filtrate, and remove the ethyl acetate solvent by rotary ev...

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Abstract

The invention provides a method for rapidly testing purity of nuciferine, belongs to the technical field of compound purity testing and in particular relates to a method for testing purity of nuciferine. The method comprises the following steps: acquiring purity of at least two known nuciferine samples; washing nuciferine with pure water; dissolving the washed nuciferines with methanol, dissolvinga methanol extract with ethyl acetate, putting an Frohde reagent into the ethyl acetate extract, carrying out a reaction, testing absorbancy A at a wavelength of 410-440nm, and drawing a standard curve equation of the absorbancy A with the purity; testing the absorbancy A of an unknown nuciferine sample, and substituting into the standard curve equation, thereby obtaining the purity of the unknown nuciferine sample. The method provided by the invention has the characteristics of being high in accuracy, simple in testing method, low in testing cost, and the like.

Description

technical field [0001] The invention belongs to the technical field of measuring the purity of compounds, in particular to a method for measuring the purity of nuciferine. Background technique [0002] Nuciferine is an aporphine-type alkaloid in lotus leaves, which is the main lipid-lowering active ingredient in lotus leaves; the dried and crushed lotus leaves are used as raw materials, pretreated with cellulase, extracted with dilute hydrochloric acid, Ultrasonic-assisted extraction and chloroform extraction are extracted by a series of methods. Among them, the lotus leaf in Shicheng, Jiangxi Province contains the highest content of nuciferin. [0003] Wang Yuxia and others studied the extraction and purification process of lotus leaf alkaloids in order to further develop the pharmacological activity of lotus leaf alkaloids extract and pharmacokinetics in vivo, and lay the foundation for its large-scale industrial production and clinical application. Methods Acid dye colo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 吴升红
Owner 安徽粮食工程职业学院
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