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Method for acquiring specific expressed genes of peanut seeds in whole genome scale and application thereof

A whole-genome, gene-expression technology, applied in biochemical equipment and methods, chemical instruments and methods, microbial measurement/inspection, etc., can solve problems such as peanut seed-specific expression genes, reduce sequencing costs and improve accuracy The effect of reducing the cost of experiments

Inactive Publication Date: 2018-10-26
SHANDONG PEANUT RES INST
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Problems solved by technology

However, only known genes can detect expression differences using this method
[0006] At present, there is no report on genome-scale screening of genes specifically expressed in peanut seeds

Method used

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  • Method for acquiring specific expressed genes of peanut seeds in whole genome scale and application thereof
  • Method for acquiring specific expressed genes of peanut seeds in whole genome scale and application thereof
  • Method for acquiring specific expressed genes of peanut seeds in whole genome scale and application thereof

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Embodiment 1

[0029] 1. Material collection

[0030] In May 2016, the cultivated peanut "Huayu 9610" was planted in the Laixi Experimental Field of the Peanut Research Institute of Shandong Province. From May to September, the roots of the peanuts at the germination and emergence stage, the stems at the seedling stage, the leaves at the flowering and needle stage, and the The flowers at the pod stage and the needles, shells, and needles at the mature stage of full fruit are buried in the soil for 10 days, 20 days, 30 days, 40 days, 50 days, 60 days, and 70 days. Seeds are frozen in liquid nitrogen and stored at -80°C Save for later.

[0031] 2. Transcriptome sequencing and result analysis

[0032]The RNAs of the above-mentioned tissues were extracted respectively, and the RNAs of peanut seeds 10 days, 20 days, 30 days, 40 days, 50 days, 60 days and 70 days were mixed as sample 1; The stems at the seedling stage, the leaves at the flowering and needle stage, the flowers at the pod stage, t...

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Abstract

The invention provides a method for acquiring specific expressed genes of peanut seeds in a whole genome scale and application thereof. According to the method provided by the invention, the specificexpressed genes of the peanut seeds can be screened in the whole genome scale, and the specific expressed genes of the peanut seeds can be arranged according to the magnitude of expression quantities;the method is capable of dividing plant tissues into seed tissues and non-seed tissues, so that the expenditure for transcriptome sequencing is greatly reduced, and the accuracy of acquiring the specific expressed genes of the peanut seeds is improved. The application of the method for acquiring the specific expressed genes of the peanut seeds in the whole genome scale in identifying specific expressed promoters of the peanut seeds is beneficial for screening and acquiring the specific expressed promoters of the peanut seeds in the genome scale. The method and the application, provided by theinvention, have important application value on cloning and identification of specific promoters of high-expression seeds of peanuts and other plants and also have important application value in acquisition of the specific expressed genes of other tissues of the peanut and the specific expressed genes of other plant tissues.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for obtaining genes specifically expressed in peanut seeds on a genome-wide scale and an application thereof. Background technique [0002] Peanut is an important oil crop in the world. The protein content in its kernel is 24%~36%; the oil content is as high as 38%~60%. It is an important source of edible protein and edible vegetable oil. In addition, peanuts are also rich in plant sterols, resveratrol, vitamin E, vitamin C and folic acid and other plant active substances, which are very beneficial for promoting health and preventing diseases. With the development of biotechnology, it has become a very effective way to use genetic engineering technology to genetically improve the protein, oil and other nutrients in peanut kernels or to use peanut kernels as "bioreactors" to synthesize protein vaccines. means. [0003] Peanut seed-specific promoter, as a kind of...

Claims

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Application Information

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IPC IPC(8): C12Q1/6869C12N15/113
CPCC07K14/415C12Q1/6869C12Q2535/122C12Q2531/113
Inventor 孙全喜苑翠玲王传堂王秀贞唐月异吴琪王志伟
Owner SHANDONG PEANUT RES INST
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