Pig 50K liquid phase chip based on targeted capture sequencing, and application thereof

Abstract

The invention relates to a pig 50K liquid phase chip based on targeted capture sequencing, and application thereof. The pig 50K liquid phase chip is composed of pig 50K probe mixed liquor and a hybridization capture reagent which are independently packaged; pig 50K probes are DNA double-stranded probes and are nucleotide sequences designed and synthesized according to screened SNP sites; and the SNP sites are screened by comparing the sequencing results of the whole genome of the pig to a pig reference genome. The pig 50K liquid phase chip prepared by the invention is mixed with a pig DNA high-throughput sequencing library; DNA fragments containing target sites in the pig DNA high-throughput sequencing library are captured for amplification and purification; and, after a product is subjected to high-throughput sequencing, a sequencing result is replied to a pig reference genome for comparison, so that the genotyping of the genome of a to-be-detected pig is obtained. When the 50K liquid phase chip prepared by the invention is used for genotyping pigs; and the problems of high cost, inflexibility and incapability of large-scale use in pig farms in China in the prior art can be solved.

Description

technical field [0001] The invention relates to the technical field of genotyping, in particular to a porcine 50K liquid phase chip based on genotyping by Targeted Sequencing (GBTS) technology and its application to genotyping the porcine genome. Background technique [0002] Single nucleotide polymorphism (SNP) has the characteristics of large number, wide distribution, easy rapid and large-scale screening, and convenient genotyping. Satellites are the third generation of genetic markers after simple tandem repeat markers, which are considered to be the best marker selection at present and have important biological significance. At present, SNP markers have become an important tool for biological population identification, genetic structure analysis, functional gene mapping, and genome selection. With the development of high-throughput SNP genotyping technology, methods based on whole genome or simplified genome sequencing have become the mainstream of SNP genotyping techn...

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Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a porcine 50K liquid phase chip based on targeted capture sequencing and its application, and also provides a special SNP probe design principle and a pig whole genome 50K liquid phase chip, using this method It can solve the problems of high cost, inflexibility and inability to be used on a large scale in pig farms in my country

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