A quantum dot-labeled antibody probe test strip and its preparation method and application

Abstract

The invention belongs to the field of chemical detection, relates to a visualized and rapid detection method for detecting residual amount of benzothiostrobin in strawberries, and relates to pesticideresidue detection, in particular to a quantum dot-labeled antibody probe test strip as well as a preparation method and application thereof. The quantum dot-labeled antibody probe test strip comprises a bottom plate, a nitrocellulose membrane, a sample pad, a bonding pad and an absorption pad, wherein the nitrocellulose membrane is located on the bottom plate; the sample pad and the bonding pad are sequentially arranged on the bottom plate on the left side of the nitrocellulose membrane, and the absorption pad is arranged on the bottom plate on the right side of the nitrocellulose membrane; quantum dot-labeled antibody probes are distributed on the bonding pad; and benzothiostrobin antigens and goat-anti-mouse antibodies are further distributed on the nitrocellulose membrane. The invention provides the visualized and rapid detection test strip which is suitable for quantitatively detecting the residual amount of the benzothiostrobin in strawberries.

Description

technical field [0001] The invention belongs to the field of chemical detection, and relates to a visual and rapid detection method for detecting benzathystrobin residues in strawberries, to the detection of pesticide residues, and in particular to a quantum dot-labeled antibody probe test strip and its preparation method and application. Background technique [0002] In the past five years, the proportion of fungicides in the total amount of pesticides has increased rapidly, and the potential toxicity risks brought by them have continued to increase. In particular, the research on new methods for on-site rapid detection of fungicide residues in raw fruits has attracted much attention. Methoxyacrylate fungicides (strobilurins) are currently one of the most widely used fungicide varieties, and the sales of its representative variety, azoxystrobin, reached US$1.4 billion in my country in 2014. Benzothiostrobin (test code Y5247, benzothiostrobin) is a candidate methoxyacrylate ...

AI Technical Summary

Problems solved by technology

[0010] The present invention proposes a quantum dot-labeled antibody probe test strip and its preparation method and application, which solves the problem that the conventional detection methods of benzathystrobin in food in the prior art are high performance liquid chromatography, ELISA, etc. Although these instrumental analysis methods are reliable, they require professional technicians and high testing costs. They are not suitable for daily testing of commonly used fruits with short shelf life and scattered sites, and are not conducive to the source supervision and quality safety of fruit pesticide residues.

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