Method for establishing laser wound animal model

Abstract

The invention discloses a method for establishing a laser wound animal model. The method includes conducting laser radiation on the back of an animal, wherein the laser irradiation conditions are: frequency 1-10P / S, energy 100-2000mJ, irradiation times 20-40 times, irradiation distance 5-25cm and time interval 1-10s. The laser radiation conditions can be adjusted to obtain an animal model with a shallow first degree wound, a shallow second degree wound, a deep second degree wound or a third degree wound. The method is simple in operation, good in repeatability and high in model success rate, shortens the time for preparing the animal model, provides a theoretical reference for related wound researches, and lays a foundation for the wound research of medical staff and medical cosmetology plastic surgery personnel.

Description

technical field [0001] The invention belongs to the field of biomedical wound repair, in particular to a method for establishing an animal model of laser trauma. Background technique [0002] The preparation methods of existing wound models mainly include: 1) full-thickness skin excision wound model; 2) skin linear cut wound model; 3) burn wound healing model; 4) scald wound model; 5) local skin necrosis model; 6) Infected wound animal model; 7) Radiation combined injury animal model; 8) Corrosive chemical reagent induced local skin trauma model. Among the existing trauma models, patents related to trauma models are as follows: CN107016916A invention patent application, using TPE to make a human body model, and simulating scratches by coating red liquid; CN107334559A invention patent application, cutting rat lumbar intervertebral discs to establish rupture Animal model of lumbar intervertebral disc herniation; CN107440812A invention patent application, the optic nerve injur...

AI Technical Summary

Problems solved by technology

[0003] Its modeling method of existing wound animal model mainly has the following deficiencies: (1) most of the models use high-energy objects to scald the animal surface, and it is difficult to control the temperature of the scalded object surface and the grasp of the scalding time; (2) the model Most of the skin epidermis excision is used, which will easily lead to deeper skin trauma, causing the animal epidermis to be directly exposed to the air, increasing the chance of infection; (3) most of the models use highly corrosive chemical substances to act on the animal skin surface for a certain period of time or Local injection of drugs that can cause tissue necrosis, which can easily lead to animal dermis necrosis and ulcer formation

[0004] In the existing trauma model preparation, no laser is used as a trauma instrument to prepare a trauma model. After searching the literature and related patent databases, no research or patent application identical or similar to this study was found. Therefore, a laser irradiation method was used to construct a trauma model. Trauma animal models with simple operation, good repeatability and high model success rate are of great significance in superficial skin wound repair and medical aesthetic plastic surgery

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