Application of medical composition in preparation of drug for preventing or treating ischemia retinae
A pharmaceutical composition, retinal ischemia technology, applied in the direction of drug combination, pharmaceutical formula, medical preparations containing active ingredients, etc., can solve problems such as poor visual results
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Embodiment 1
[0032] Example 1. The effect of Xuefu Zhuyu Decoction on the b-wave of retinal electrogram.
[0033] The experimental animals were handled in accordance with the Association for Research in Vision and Ophthalmology Statement for the Use of Animals in Ophthalmology and Vision Research, and all animal experiments were approved by Zhenxing Hospital Animal Care and Use The committee agreed (CHGH; Taipei, Taiwan; approval number: CHIACUC 104-05). Six-week-old male Wistar strain rats (BioLasco, Taipei, Taiwan) were housed in large plastic cages (Shineteh Instrument Co., Ltd. ). Rats are arbitrarily assigned to one group in the following groups, and the experimental animals (the number of samples is 120) are used in various experimental methods, that is, 3 groups of electroretinogram analysis [the number of samples is 16 in total, including: normal group (sample number number is 5); I / R+vehicle group (sample number is 5); I / R+Xuefu Zhuyu Decoction 1.35 group (the number of samples...
Embodiment 2
[0040] Example 2. Effect of Xuefu Zhuyu Decoction on the density of RGCs labeled with retrograde fluorescence.
[0041] After anesthesia, a 2 cm incision was made in the rat scalp and two small holes were drilled in the skull as described in previous techniques. (Peng PH, Chao HM, Juan SH, Chen CF, Liu JH and Ko ML.Pharmacologicalpreconditioning by low dose cobalt protoporphyrin induces heme oxygenase-1overexpression and induces retinal ischemia-reperfusion injury inrats.Curr.Eye Res.36(3):238 -246, 2011). Next, 2 microliters of 5% gold fluoride (Sigma-Aldrich, MO, USA) were injected via a micropipette at depths of 3.8, 4.0 and 4.2 mm below the skull. In all groups, gold fluoride was injected three days before sacrificing the rats. Retina were gently removed, fixed, dissected and processed as described in previous art. (PengPH, Chao HM, Juan SH, Chen CF, Liu JH and Ko ML.Pharmacological preconditioning by low dose cobalt protoporphyrin induces heme oxygenase-1overexpression...
Embodiment 3
[0043] Example 3. The effect of Xuefu Zhuyu Decoction on the thickness of various retinal layers stained with cresyl violet.
[0044] Animals were sacrificed and intracardiacly perfused with saline (weight volume concentration), the eyeballs were removed, fixed with 4% (weight volume concentration) paraformaldehyde and embedded in paraffin (Tissue-Tek TEC 5; Sakura, Alphen aan denRijn , Netherlands) and sliced (5 microns). Retinal sections were stained with cresyl violet and examined under a light microscope (Leica, Heidelberg, Germany). Retinal sections were examined under a microscope and photographed at the same magnification (Ilford Pan-F plus film, 50ASA), and various retinal layer thicknesses were calculated from the photographs.
[0045] Whole-body retinal thickness (from inner limiting membrane to retinal pigment epithelium) and inner retinal thickness (from inner limiting membrane to inner nuclear layer) were measured to quantify retinal ischemic damage. All measu...
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