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A method for constructing the HPLC characteristic map of Jingqi Shuangshen Capsules

A construction method and characteristic map technology, applied in the field of medicine, can solve the problems of no relevant reports on the construction method, respond to product quality, control product quality, etc., and achieve the effect of scientific reliability, good reproducibility, and good separation effect

Active Publication Date: 2020-11-13
吉林修正药业新药开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the patents related to quality control are only for the content determination and qualitative identification of an active ingredient of a blind drug, and it is difficult to reflect the quality of the product as a whole or to control the quality of the product as a whole
At present, there is no relevant report on the construction method of the HPLC characteristic map of Jingqi Shuangshen Capsules

Method used

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  • A method for constructing the HPLC characteristic map of Jingqi Shuangshen Capsules
  • A method for constructing the HPLC characteristic map of Jingqi Shuangshen Capsules
  • A method for constructing the HPLC characteristic map of Jingqi Shuangshen Capsules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1: A method for constructing the HPLC characteristic spectrum of Jingqi Shuangshen Capsules

[0056] Instruments: UltiMate 3000 HPLC, UV DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.), etc.

[0057] Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0058] Reagent: Ginsenoside Rg 1 , salvianolic acid B, formononetin and tanshinone IIA (all purchased from China National Institutes for Food and Drug Control).

[0059] Method and Results

[0060] (1) Preparation of the test solution: Take about 1 g of the contents of Jingqi Shuangshen Capsules, accurately weighed, accurately add 10 mL of methanol, weigh the weight, ultrasonically treat for 20 minutes, let it cool, and make up the lost weight with methanol, Filtrate, take the continued filtra...

Embodiment 2

[0066] Example 2: A method for constructing the HPLC characteristic spectrum of Jingqi Shuangshen Capsules

[0067] Instruments: UltiMate 3000 HPLC, UV DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.), etc.

[0068] Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0069] Reagent: Ginsenoside Rg 1 , salvianolic acid B, formononetin and tanshinone IIA (all purchased from China National Institutes for Food and Drug Control).

[0070] Method and Results

[0071] (1) Preparation of the test solution: Take about 2 g of the contents of Jingqi Shuangshen Capsules, accurately weighed, accurately add 25 mL of methanol, weigh, ultrasonically treat for 30 minutes, let cool, make up the lost weight with methanol, Filtrate, take the continued filtrate, and obtain;

...

Embodiment 3

[0075] Example 3: A method for constructing the HPLC characteristic map of Jingqi Shuangshen Capsules

[0076] Instruments: UltiMate 3000 HPLC, UV DAD detector; Mettler AE240 1 / 100,000 analytical balance (Shanghai Mettler-Toledo Instrument Co., Ltd.); AB204-E electronic balance (Shanghai Mettler-Toledo Instrument Co., Ltd.), etc.

[0077] Reagents: methanol, etc. are analytically pure, phosphoric acid and acetonitrile are chromatographically pure, and water is ultrapure water.

[0078] Reagent: Ginsenoside Rg 1 , salvianolic acid B, formononetin and tanshinone IIA (all purchased from China National Institutes for Food and Drug Control).

[0079] Method and Results

[0080] (1) Preparation of the test solution: take about 3 g of the contents of Jingqi Shuangshen Capsules, accurately weighed, accurately add 50 mL of methanol, weigh, and ultrasonically treat for 40 minutes, let it cool, and make up the lost weight with methanol, Filtrate, take the continued filtrate, and obta...

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Abstract

The invention relates to the technical field of medicines, in particular to a construction method for an HPLC specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules. The construction method comprises the following steps: taking the content of the fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules, adding methanol, filtering the mixture after ultrasonic treatment, and taking a continuous filtrate to obtain a solution of a sample for test; taking a ginsenoside Rg1 reference substance, a danshinolic acid B reference substance, a formononetin reference substance and a tanshinone IIA reference substance to be mixed, and adding methanol to obtain a reference substance solution; testing the solution of the sample for test and the reference substance solution by means of high performance liquid chromatography separately to obtain the HPLC specific chromatogram of the fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules, wherein the chromatographic condition of the high performance liquid chromatography is as follows: by taking a C18 column as a chromatographic column, acetonitrile as a moving phase A and a 0.1%phosphoric acid solution as a moving phase B, gradient elution is carried out. The specific chromatogram of fine astragalus mongholicus ginseng and salviae miltiorrhizae capsules established by the method is relatively good in separating degree, and meanwhile, 15 active components of traditional Chinese medicines can be separated and detected at the same time.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a method for constructing an HPLC characteristic map of Jingqi Shuangshen Capsules. Background technique [0002] Jingqi Shuangshen Capsule is a traditional Chinese medicine compound composed of 4 flavors of astragalus, ginseng, salvia miltiorrhiza and sealwort. It is suitable for heart palpitations, shortness of breath, dizziness and headache, fatigue, tinnitus, numbness of limbs, loss of eyesight and forgetfulness, etc. due to cardiovascular and cerebrovascular diseases. The current national drug standard number is WS-5136(B-0136)-2002. The standard only contains the characteristics, inspection and identification items and content determination items of individual medicinal materials. Because the traditional Chinese medicine compound preparation has many medicinal flavors and complex components interfere with each other, it is not easy to be effective. Testing and other chara...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 吴萍徐建白冰李亚东崔宪利
Owner 吉林修正药业新药开发有限公司