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Panax ginseng adventitious root inducing culture medium and application thereof

A technology for inducing culture medium and culture medium, applied in the field of ginseng adventitious root induction medium, can solve the problems of low ginseng adventitious root saponin content, poor cell genetic stability, etc., and achieve the effects of solving the shortage of ginseng, shortening the production cycle and high stability

Inactive Publication Date: 2018-11-06
刘汉石
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a method for inducing adventitious roots of ginseng to solve the problems of low saponin content and poor cytogenetic stability in artificially induced ginseng adventitious roots in the prior art

Method used

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  • Panax ginseng adventitious root inducing culture medium and application thereof
  • Panax ginseng adventitious root inducing culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Preparation of ginseng callus induction medium

[0036] MS basal medium was prepared (see Table 1 for the formula), and 0.5 mg / LGA (gibberellin), 30 g / L sucrose, and 0.6 g / L agar were added to the MS basal medium. After the prepared medium was sterilized at high temperature, it was cooled and poured into a plate for use.

Embodiment 2

[0037] Example 2 Preparation of ginseng cell culture medium

[0038] To prepare MS basal medium (see Table 1 for the formula), add 0.5mg / L 6-BA (6-benzylpurine), 4mg / L NAA (naphthalene acetic acid), 0.8g / L agar and 30g to the MS basal medium / L sucrose. After the prepared medium was sterilized at high temperature, it was cooled and poured into a plate for use.

Embodiment 3

[0039] Example 3 Preparation of adventitious root induction medium

[0040] To prepare MS basal medium (see Table 1 for the formula), add 0.4mg / L 6-BA (6-benzylpurine), 3mg / L NAA (naphthalene acetic acid), 0.7g / L agar and 25g to the MS basal medium / L sucrose. After the prepared medium was sterilized at high temperature, it was cooled and poured into a plate for use.

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Abstract

The invention provides a panax ginseng adventitious root inducing culture medium and a method for inducing panax ginseng adventitious root. The panax ginseng adventitious root inducing culture mediumcomprises an MS basic culture medium, 0.2 mg / L-0.5 mg / L of IBA (indolebutyric acid), 2 mg / L-4 mg / L of NAA (naphthaleneacetic acid) and 20 g / L-30 g / L of sucrose. The panax ginseng adventitious root prepared by the method of the invention has a high content of ginsenoside, stable panax ginseng cell heredity and little influence by environmental factors, can be used as an ideal model for ginsenosidebiosynthesis molecule regulation research, and can be used for exploring an effective route for large-scale production of ginsenoside by engineered cells.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a ginseng adventitious root induction medium and application thereof. Background technique [0002] Ginseng, a medicinal plant, is a traditional precious Chinese medicinal material in my country and is known as the king of medicine. Its products have a broad market. Panax ginseng C.A.Meyer is a plant of the Araliaceae family, mainly distributed in Northeast China, Korea and Japan. Its secondary metabolite, ginsenoside, is a tetracyclic triterpene dammarane compound composed of isoprene units (five carbons). for stems and leaves. So far, more than 50 kinds of saponins have been isolated and their structures have been determined. Modern research believes that the main medicinal and active ingredients of ginseng are ginsenosides, which not only have pharmacological effects on the central nervous system, cardiovascular, immune and endocrine systems, but also exhibit various ac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘汉石刘禹
Owner 刘汉石
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