52results about How to "High in saponins" patented technology

The invention relates to a method for separating and purifying sanguisorba saponins and sanguisorbin I by using alkaline liquid medicine through macroporous resin, comprising the following steps of: carrying out hot reflux extraction or percolation on cut-off sanguisorba, which is a traditional Chinese medicine and used as a raw material, more than one time with water or alcohol/water, and processing extracts by using alkali to regulate pH value to 10-15; adsorbing and separating the filtered extracts by using the macroporous resins, eluting the extracts by using alkaline water or water and about 30% of alcohol to remove impurities, and then desorbing and eluting by using organic solvents; carrying out decompression concentration, concentration, standing and filtration on eluent, and thencollecting sediments to obtain the sanguisorba saponins; or directly sedimentating by using the alkali, regulating the pH of the filtered liquid medicine to 4-6 after filtration, concentrating to recover alcohol; standing to separate out and obtain a sanguisorba saponins mixture, and washing the eluted resins by using the organic solvents for repeated use; and recrystallizing the sanguisorba saponins mixture by using alcohol water to obtain the sanguisorbin I with the content more than 98%. Saponins and other impurities are separated and extracted in the method, and the method has the advantages of low cost, high efficiency, simplicity, convenience and suitability for industrial production and can be used for obtaining high-purity sanguisorba saponins and sanguisorbin I.

The invention relates to a method for processing a notoginseng decoction piece, which mainly comprises the steps of sorting, cleaning, airing, pruning, slicing and drying fresh notoginsengs, wherein the thickness of the slices is between 0.5 and 5 centimeters, the drying method adopts infrared rays, microwaves or steam, and the drying temperature is between 60 DEG C below zero and 120 DEG C. The method can ensure that the saponin content of the notoginseng decoction piece is 0.5 to 2 percent higher than that of the conventional processing method, reduce the processing production cost and improve the production efficiency; besides, the method performs slicing processing on the fresh notoginsengs for the first time in the process of processing the notoginsengs, and introduces devices to perform drying processing on the notoginseng slices for the first time.

The invention discloses a bioreactor for cultivating ginseng adventitious roots by dynamic balance alimentation. The bioreactor comprises a primary culture medium reaction tank, an air pressure pump and a nutrition additive solution tank, wherein the primary culture medium reaction tank is a bubbling bioreaction tank; a nutrition additive solution dropping opening is also formed in a reaction tank cover; a material outlet with an adjustment valve is formed in the bottom of the nutrition additive solution tank; a tank cover is arranged at the top of the nutrition additive solution tank, and an air inlet with a filter is formed in the top of the nutrition additive solution tank; the nutrition additive solution dropping opening is connected with the material outlet through a pipeline. The invention further provides a system for producing the ginseng adventitious roots by the bioreactor. The system consists of a nutrition additive solution tank and three primary culture medium reaction tanks. The bioreactor can drop a nutrition additive solution at proper time, so that the saponins content is increased; particularly, a culture medium with high-concentration saccharose in the nutrition additive solution tank can be used for dynamically balancing the saccharose concentration in the reaction tank, so that the adding amount of saccharose can be increased, and the yield of the adventitious roots can be increased; the problem of suppression, which is caused by high saccharose concentration, on the growth of the adventitious roots is solved.

The invention provides a ginseng cell induction medium and a ginseng cell induction method. The ginseng cell induction medium comprises an MS basic culture medium, 0.2-0.5mg/L of 6-BA (6-benzyladenine), 2-4mg/L of NAA (naphthylacetic acid) and 20-30g/L of sucrose. Ginseng cells prepared by the method are high in ginsenoside content and stable in heredity, influence of environment factors on the ginseng cells is low, the ginseng cells can be used as the ideal model of ginsenoside biological synthesis molecular regulation researches, an effective approach for achieving large-scale industrial production of the ginsenoside using the ginseng cells can be explored, and the method is worthy of popularization and application.

The invention discloses a method for culturing ginseng stem cells by using a biological reaction device. The method comprises the following steps: (1) preparing ginseng adventitious roots by adopting a one-step method; (2) taking a root tip part of each ginseng adventitious root prepared in the step (1), and treating the root tip part in a penetrant solution; (3) putting the root tips of the ginseng adventitious roots treated by the penetrant solution in the step (2) into a stem cell induction culture medium for culture, and then transferring into a propagation culture medium for culture; (4) inoculating the ginseng stem cells propagated and cultured in the step (3) into a stem cell liquid culture medium shake flask, and culturing in a dark place under the condition of 100-140 rpm; (5) inoculating the ginseng stem cells subjected to shake-flask culture in the step (4) into a biological reaction device, and culturing in a dark place to obtain the ginseng stem cells; The method for obtaining the ginseng stem cells by obtaining the adventitious roots from the mature ginseng with shorter required time and simpler steps is obtained, an appropriate biological reaction device is adopted for amplification culture, the growth multiple of the stem cells is high, and the content of effective components in the stem cells is high.

The invention discloses a method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors. The method comprises the following steps: picking the ginseng adventitious roots which grow well and inoculating into a modified 3/4 MS liquid culture medium; culturing for 28-30 days; adding a lactoalbumin hydrolysate water solution mother solution and a jasmonic acid methyl ester ethanol mother solution which are used as the inductors, wherein the final concentration of lactoalbumin hydrolysate is 98mg/L-100mg/L and the final concentration of jasmonic acid methyl ester is 8mg/L-10mg/L; and continuing to culture for 2-7 days, harvesting and drying. An experiment shows that the two inductors including lactoalbumin hydrolysate and jasmonic acid methyl ester can express the synergistic effect and do not have influences on secondary metabolism of the ginseng adventitious roots; the content of the saponin is high and evidences are provided for the large-scale production of ginsengs; and the industrial production of the ginseng saponin is realized and the day-by-day increasing requirements of people are met.

The invention relates to a microwave vacuum-hot air sectional type drying method for pseudo-ginseng, and belongs to the technical field of pseudo-ginseng drying. The method comprises the following steps of: 1) carrying out pseudo-ginseng drying early-stage preparation work: storing, cleaning and classifying; 2) determining the initial wet basis moisture content of pseudo-ginseng materials; (3) setting drying conditions of a microwave vacuum dryer, and drying the pseudo-ginseng similar materials by adopting an intermittent drying method under the conditions, so that the wet basis moisture content of the pseudo-ginseng similar materials is reduced to the converted wet basis moisture content; and 4) drying the pseudo-ginseng similar materials obtained in the step 3) again by adopting a hot air drying method, so that the wet basis moisture content of the pseudo-ginseng similar materials is reduced to the final safe wet basis moisture content; Microwave vacuum-hot air sectional type combined drying is adopted, the pseudo-ginseng drying time is effectively shortened, the drying rate is increased, and the dried pseudo-ginseng finished product is high in saponin content, safe and sanitary.

The invention discloses a walnut-perilla-fish oil capsule for preventing and treating memory decline and insomnia. The capsule comprises the following components: 12-15 parts of walnut oil, 5-6 partsof fish oil, 5-8 parts of wheat germ powder, 5-10 parts of perilla oil, 10-15 parts of fructus alpiniae oxyphyllae, 5-8 parts of corn starch, 8-10 parts of skimmed milk powder, 5-8 parts of rhizoma acori graminei, 5-8 parts of fructus lycii, 5-8 parts of fructus schisandrae chinensis, 5-8 parts of lilii bulbus, 5-8 parts of American ginseng ferment powder, 3-5 parts of ginkgo leaf, 2-3 parts of lysine, 1-2 parts of leucine, 3-5 parts of monoammonium glycyrrhizinate, 2-3 parts of Chinese date and 3-4 parts of xylooligosaccharide. According to the capsule, the American ginseng ferment powder with high saponin content, the monoammonium glycyrrhizinate and other components containing brain-invigorating and intelligence-benefiting components are reasonably compounded, so that the capsule is suitable for teenagers, high-strength mental workers and middle-aged and old-aged people with memory decline, and has effects of invigorating the brain and benefiting intelligence, calming the heart andtranquilizing the mind, and improving the memory.

The invention discloses an isolated culture method of ginseng stem cells. The isolated culture method comprises the following steps: (1) preparing ginseng adventitious roots by adopting a one-step method; (2) taking the root tip part of the ginseng adventitious root prepared in the step (1), and dissecting and separating to obtain a stem cell region; (3) inoculating the stem cells obtained by separation in the step (2) into a stem cell induction medium for dark culture to obtain a stem cell mass, and then transferring the stem cell mass into a subculture medium for dark culture; and (4) inoculating the stem cells subjected to subculture in the step (3) into a stem cell liquid culture medium, and performing dark culture to obtain the ginseng stem cells. By adopting the liquid culture medium and the culture method disclosed by the invention, adventitious roots can be directly generated through induction of each part of mature ginseng, the content of ginsenoside in the adventitious roots is increased while the induction steps are simplified and the induction time is shortened, and finally ginseng stem cells with good indexes are obtained.

The invention provides a method for inducing ardisia crenata to generate hairy roots by efficiently utilizing agrobacterium rhizogenes and belongs to the technical field of plant tissue culture. The method comprises the following steps: (1) activation of the agrobacterium rhizogenes: (2) induction of the hairy roots of sterile leaves in tissue culture of ardisia crenata; (3) hairy root subculture.The method has the advantages that the method is simple in operation, capable of saving time and high in efficiency, the induction rate of the hairy roots of the ardisia crenata is high, the hairy roots of the ardisia crenata with high ardisia crenata saponin content can be obtained, the culture time of the ardisia crenata can be obviously shortened, technical support and a scientific basis are provided for industrial production, and ecological environment protection is facilitated.

The invention discloses a dried pseudo-ginseng finished product, a drying process of the product and a parameter determination method of the drying process. By weight, the content of total saponins inthe dried pseudo-ginseng product is larger than or equal to 6.803%, and the moisture content is smaller than or equal to 10.343%, wherein the total saponins comprise ginsenoside Rg1, ginsenoside Rb1,ginsenoside Re, ginsenoside Rd and notoginsenoside R1, the content of ginsenoside Rg1 is larger than or equal to 2.988%, the content of ginsenoside Rb1 is larger than or equal to 1.695%, the contentof ginsenoside Re is larger than or equal to 0.299%, the content of ginsenoside Rd is larger than or equal to 0.528%, and the content of notoginsenoside R1 is larger than or equal to 0.744%. The drying process comrises the following steps: (1) sorting fresh pseudo-ginseng; (2) removing large roots, fibrous roots and sheared edges; (3) cleaning; (4) refrigerating; (5) slicing; (6) hot air drying; and (7) finished product obtaining. The pseudo-ginseng product is high in saponin content, low in water content and good in antioxidant activity, and the drying process is short in drying time and highin drying efficiency.

The invention relates to a hyacinth bean sauce which is rich in saponin and high protein and a preparation method thereof in the technical field of processing of agricultural products. The hyacinth bean sauce comprises the following components in percentage by weight: 60 percent of fresh hyacinth bean podwall sauce liquid, 6.9 percent of broken peanut, 5-6 percent of soyabean protein powder with protein content of more than 90 percent, 2.0-3.5 percent of guar gum, 1.0-2.5 percent of konjac flour, 0.6-0.8 percent of chitin, 0.3-0.5 percent of bone meal, 2-4 percent of salt, 12-14 percent of white granulated sugar and 5.5-6.5 percent of maltose. The invention solves the problem that a hyacinth bean product scarcely has nutrients and nutrient components, such as high saponin, protein, and the like and has simple and convenient implementation and the effect of greatly improving the yield and the quality of a hyacinth bean processed product.

The invention relates to a method for preparing full-price ginseng by using combined ginseng leaves and ginseng roots, which is characterized in that a concentrated solution which is extracted by mixing sun-dried ginseng with the ginseng leaves and gynostemma pentaphylla is used for continuous soaking and freeze-drying for three times, and finally, drying, sterilizing and packaging are performed, so that the full-price ginseng is obtained. The repeated physical effect provides full conditions for the sun-dried ginseng to fully absorb and change effective components in the ginseng leaves and the gynostemma pentaphylla, so that the febrile dryness of the sun-dried ginseng is gently changed under the frozen physical action and the cold chemical action of the ginseng leaves, the saponin content is obviously increased, the full-price ginseng is suitable for more people to tolerate, and the application value of the ginseng is increased.

The invention relates to the field of traditional Chinese medicinal material planting and discloses an under-forest tending method for pseudo-ginseng seedlings. The method is technically characterized by including the steps of seedling culture, screening, environment selection, forest land cleaning, punch transplanting, later management and the like; pseudo-ginseng seeds are cultured into the pseudo-ginseng seedlings for transplanting, and soil disinfection serves as the key during transplanting; by means of the tending method, a new thought is provided for obtaining higher-quality pseudo-ginseng, the pseudo-ginseng seeds are cultured into the seedlings for under-forest tending, the under-forest tending progress of the pseudo-ginseng is accelerated, one year for developing the seeds into the seedlings is shortened, disinfection is carried out during seed culture, the problem of rust diseases during seedling development can be greatly relieved, the rust disease incidence during seedling development in the prior art is 20% to 30%, and the rust disease incidence at present is reduced to 5% to 10%; the proper altitude, gradient, under-forest environment and soil environment are selected, and the content of panax notoginseng saponins is increased by about 3%. The problems that in the prior art, the survival rate of pseudo-ginseng under-forest tending is low, and the influences of diseases are high are solved.

The invention discloses a processing method for improving a saponin component in paris polyphylla. The method comprises the following steps: digging the paris polyphylla which is beaten down in autumn, cleaning sediments on the surface, cutting into slices, and rapidly drying in a drying oven at 40-80 DEG C; taking out when the moisture reaches the medicinal material standard below 14%; and cooling to a room temperature and bagging to be used as a medicinal material. According to the processing method, the moisture in fresh paris polyphylla can be rapidly evaporated within a short period of time; hydrolysis or degradation of the effective component paris polyphylla saponin in a micro-environment can be avoided; compared with a medicinal material drying method in the paris polyphylla item in the Chinese pharmacopoeia, the saponin content of the medicinal material paris polyphylla can be effectively improved to over 30%, and can be up to over 100%; the content of the effective components of the medicinal material is improved; the content of the effective components of the paris polyphylla is improved; the internal and external quality of the medicinal material is improved; the method is simple to operate and low in cost, and is a completely physical method; and no chemical agent is used, so that the toxic and side effects of the medicinal material are not increased.

The invention provides an aquaculture method capable of improving saponin content of sea cucumbers. The method includes: adding glucose, xylose, galactose, rhamnose, arabinose, glucuronic acid, and galacturonic acid into cultivation sea water; and effectively controlling the cultivation temperature, the pH, the concentration of iron element, and the illumination so as to remarkably promote accumulation of saponin ingredients in the sea cucumbers. Compared with the prior art, the aquaculture method can improve the activity of non-specific immune factors in the obtained sea cucumbers, can enhance the disease resistance of the sea cucumbers, can facilitate healthy growth of the sea cucumbers, and then increases the yield of the sea cucumbers. The aquaculture method can remarkably improve the saponin content of the sea cucumbers through reasonable design of the process, the nutriment value of the obtained sea cucumbers is high than that of the common sea cucumbers, the cost is not increased, and the obtained sea cucumbers have high commercial value.

The invention relates to cladosporium tenuissimum as the fungi with the probiotic effect to panax notoginseng and the capability of increasing the content of notoginsenoside as well as an inoculant ofthe cladosporium tenuissimum and belongs to the technical field of biology. The bacterial strain is separated from stems of healthy biennial panax notoginseng, is authenticated to be the cladosporiumtenuissimum and is preserved in China General Microbiological Culture Collection Center with a preservation number being CGMCC No.14126. Preparation of the inoculant mainly comprises the following steps of pure culture of microorganisms, preparation of a spore suspension, production of the inoculant and application of the inoculant. The inoculant can also be used by being mixed with an organic fertilizer to prepare a bacterial fertilizer, has the probiotic effect to the panax notoginseng, can promote increase of the content of the notoginsenoside to a certain extent and shows a good ecological application prospect.