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Method for culturing ginseng stem cells by using biological reaction device

A biological reaction device, stem cell technology, applied in the methods of supporting/fixing microorganisms, bioreactors/fermenters for specific purposes, biochemical cleaning devices, etc. , limited splitting ability and other problems, to achieve the effect of shortening the induction time, uniform and sufficient ventilation, and increasing the content of active ingredients

Active Publication Date: 2021-06-25
SHANDONG ANRAN NANOMETRE IND DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of cell essence comes from differentiated somatic cells, so the ability to divide is limited and the ability to resist stress is not strong
In industrial production, cell line degeneration is prone to occur, and the ability to divide is weak
In addition, the culture period is long, and the content of active ingredients in the obtained stem cells also needs to be improved.

Method used

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  • Method for culturing ginseng stem cells by using biological reaction device
  • Method for culturing ginseng stem cells by using biological reaction device
  • Method for culturing ginseng stem cells by using biological reaction device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0102] In this example, the adventitious root of ginseng is prepared by one-step method and specific liquid medium culture, which specifically includes the following steps:

[0103] (1) Induction of adventitious roots

[0104] After 20-year-old wild ginseng, remove the reed head, stalk, and main root after cleaning, disinfection and sterilization, cut into thin slices with a width of 0.6cm, a length of 0.7cm and a thickness of 0.3mm, and inoculate them into induction medium, at 22±1°C Adventitious roots of wild ginseng were induced by dark culture for 4 to 5 weeks under conditions; wherein, the induction medium included 4mg / L naphthaleneacetic acid, 0.6mg / L gibberellin, 0.4mg / L kinetin, 0.1g / L citric acid, 0.05g / L L ascorbic acid, 30g / L sucrose, 3g / L Phytogel, 1.55g / L B5 medium and 1.21g / L WPM medium, pH value is 5.8.

[0105] (2) Subculture of adventitious roots

[0106] Inoculate the adventitious roots of wild ginseng obtained in step (1) into the same induction medium as in...

Embodiment 2

[0111] In this example, the adventitious root of ginseng is prepared by one-step method and specific liquid medium culture, which specifically includes the following steps:

[0112] (1) Induction of adventitious roots

[0113] After cleaning, disinfecting and degerming the reed heads of 6-year-old garden ginseng, cut them into thin slices with a width of 0.5 cm, a length of 0.6 cm and a thickness of 0.3 mm, inoculate them into induction medium, and culture them in dark at 22±1°C for 4-5 days. Zhou induced adventitious roots; wherein, the induction medium included 6mg / L naphthaleneacetic acid, 0.2mg / L gibberellin, 0.4mg / L kinetin, 1.2g / L citric acid, 0.1g / L ascorbic acid, 20g / L sucrose, 5g / L plant gel, 4g / L B5 medium and 1.8g / L WPM medium, pH value is 5.6.

[0114] (2) Subculture of adventitious roots

[0115] Inoculate the adventitious roots obtained in step (1) into the same induction medium as in step (1), and culture in dark under the same conditions for 4 to 5 weeks;

...

Embodiment 3

[0120] In this example, the adventitious root of ginseng is prepared by one-step method and specific liquid medium culture, which specifically includes the following steps:

[0121] (1) Induction of adventitious roots

[0122] After cleaning, disinfecting and sterilizing the horns of 10-year-old understory ginseng, cut them into thin slices with a width of 0.7 cm, a length of 0.7 cm, and a thickness of 0.5 mm, inoculate them into induction medium, and culture them in dark at 22±1°C for 4~ Adventitious roots were induced in 5 weeks; wherein, the induction medium included 5mg / L naphthaleneacetic acid, 1mg / L gibberellin, 0.1mg / L kinetin, 0.75g / L citric acid, 0.03g / L ascorbic acid, 40g / L sucrose, 4g / L Phytogel, 2g / L B5 medium and 1g / L WPM medium, pH 6.0.

[0123] (2) Subculture of adventitious roots

[0124] Inoculate the adventitious roots obtained in step (1) into the same induction medium as in step (1), and culture in dark under the same conditions for 4 to 5 weeks;

[0125...

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Abstract

The invention discloses a method for culturing ginseng stem cells by using a biological reaction device. The method comprises the following steps: (1) preparing ginseng adventitious roots by adopting a one-step method; (2) taking a root tip part of each ginseng adventitious root prepared in the step (1), and treating the root tip part in a penetrant solution; (3) putting the root tips of the ginseng adventitious roots treated by the penetrant solution in the step (2) into a stem cell induction culture medium for culture, and then transferring into a propagation culture medium for culture; (4) inoculating the ginseng stem cells propagated and cultured in the step (3) into a stem cell liquid culture medium shake flask, and culturing in a dark place under the condition of 100-140 rpm; (5) inoculating the ginseng stem cells subjected to shake-flask culture in the step (4) into a biological reaction device, and culturing in a dark place to obtain the ginseng stem cells; The method for obtaining the ginseng stem cells by obtaining the adventitious roots from the mature ginseng with shorter required time and simpler steps is obtained, an appropriate biological reaction device is adopted for amplification culture, the growth multiple of the stem cells is high, and the content of effective components in the stem cells is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for cultivating ginseng stem cells by using a biological reaction device. Background technique [0002] Ginseng (Panax ginseng C.A.Mey.) is a plant of the genus Panax of Araliaceae, distributed in China, Japan, and Korea. Its rhizome is a valuable Chinese medicinal material, known as the "King of Herbs". Ginseng is sweet, slightly bitter, and slightly warm. It has the functions of invigorating vital energy, restoring pulse and strengthening the spleen, nourishing the spleen and lung, promoting body fluid and nourishing blood, calming the nerves and improving intelligence, etc. Wound thirst, palpitations, insomnia, etc. Ginsenoside is the main active ingredient of ginseng, which has the effects of anti-fatigue, anti-aging, regulating the central nervous system, improving immunity, improving cardiovascular and cerebrovascular insufficiency, and in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04C12M1/00C12M1/12C12M1/04
CPCC12N5/0025C12N5/04C12M23/48C12M23/22C12M23/54C12M29/04C12M29/06
Inventor 刘冰高秀君闫培生张明臣
Owner SHANDONG ANRAN NANOMETRE IND DEV CO LTD
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