System for orientationally cultivating ginseng adventitious roots by dynamic balance alimentation
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A technology of dynamic balance and nutrition, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as nutritional imbalance and cultivating adventitious roots of ginseng
Active Publication Date: 2015-04-15
JILIN GREEN FOOD ENG RES INST
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[0011] The purpose of the present invention is to solve the problem of unbalanced nutrition at each stage of cultivating ginseng adventitious roots in a bioreactor, improve the yield
Method used
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Embodiment 1
[0024] Example 1 The acquisition of ginseng adventitious root
[0025] Select 6-year-old garden ginseng from Changbai Mountain (Ji'an County, Tonghua City, Jilin Province), cut into pieces, and use MS medium to induce callus, and use 3\4 SH medium + IBA5.0mg / L + sucrose 30g / L, induced adventitious roots, subcultured.
Embodiment 2
[0026] The preparation of embodiment 2 initial culture medium and nutrient supplement solution
[0027] 1. Preparation of culture medium
[0028] 1) Initial medium
[0029] 3 / 4SH medium + potassium indolebutyrate (K-IBA ) 3mg / L + sucrose 30g / L
[0030] 2) Nutrient additive solution
[0031] Nutrient additive solution (1):
[0032] In every liter of 3 / 4SH medium, add naphthaleneacetic acid (C 12 h 10 o 2 ) 0.5-0.9mg×3 / L, methyl jasmonate (C 13 h 20 o 3 ) 50-80mg×3 / L, salicylic acid 50-80 mg×3 / L, sucrose (C 12 h 22 o 11 ) 30g×3 / L.
[0033] Nutrient additive solution (2):
[0034] In every liter of 3 / 4SH medium, add naphthaleneacetic acid (C 12 h 10 o 2 ) 0.5-0.9g×3 / L, methyl jasmonate (C 13 h 20 o 3 ) 50-80g×3 / L, salicylic acid 50-80, sucrose (C 12 h 22 o 11 ) 40g×3 / L.
Embodiment 3
[0035] Example 3 Bioreactor Dynamic Balanced Nutrition Method Oriented Cultivation of Ginseng Adventitious Roots
[0036] 1) Add 12L of the initial culture medium into the 18L bubbling bioreactor, add 6L of the nutrient supplement into the liquid replenisher, and sterilize the bioreactor and the liquid replenisher at a temperature of 120°C and a pressure of 0.08Mpa1;
[0037] 2) The adventitious roots were cut into 1cm pieces and inoculated into the bioreactor;
[0038] 3) Ventilate and incubate at 23±2°C;
[0039] 4) After culturing until the 10th day, add 6L of nutrient supplement solution (1) dropwise, and finish dropping in 3-5 days;
[0040] 5) Continue culturing for 42 days to harvest adventitious roots.
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Abstract
The invention discloses a bioreactor for cultivating ginseng adventitious roots by dynamic balance alimentation. The bioreactor comprises a primary culture medium reaction tank, an air pressure pump and a nutrition additive solution tank, wherein the primary culture medium reaction tank is a bubbling bioreaction tank; a nutrition additive solution dropping opening is also formed in a reaction tank cover; a material outlet with an adjustment valve is formed in the bottom of the nutrition additive solution tank; a tank cover is arranged at the top of the nutrition additive solution tank, and an air inlet with a filter is formed in the top of the nutrition additive solution tank; the nutrition additive solution dropping opening is connected with the material outlet through a pipeline. The invention further provides a system for producing the ginseng adventitious roots by the bioreactor. The system consists of a nutrition additive solution tank and three primary culture medium reaction tanks. The bioreactor can drop a nutrition additive solution at proper time, so that the saponins content is increased; particularly, a culture medium with high-concentration saccharose in the nutrition additive solution tank can be used for dynamically balancing the saccharose concentration in the reaction tank, so that the adding amount of saccharose can be increased, and the yield of the adventitious roots can be increased; the problem of suppression, which is caused by high saccharose concentration, on the growth of the adventitious roots is solved.
Description
technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a system for directional cultivation of ginseng adventitious roots by a dynamic balanced nutrition method. Background technique [0002] Ginseng ( Panax ginseng C. A. Meyer) is a plant of the genus Panax L. of the family Araliacede. It is one of the famous "Three Treasures of Northeast China", and it is also a traditional Chinese herbal medicine in my country. It is well-known both at home and abroad. Li Shizhen, a great physician and pharmacist in the Ming Dynasty in my country, gave a detailed introduction to the efficacy of ginseng in his epic masterpiece "Compendium of Materia Medica", which established the reputation of ginseng as the "king of all herbs". Ginseng mostly grows in hillsides of coniferous forests or mixed forests with a small temperature difference between day and night and an altitude of 500-1100 meters. In my country, it is mainly distributed in...
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