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Sanguisorba saponins and preparation method of sanguisorbin I

A technology of total saponins and Burnet, which is applied in the field of medicine to achieve the effects of cost reduction, high enrichment and low price

Active Publication Date: 2010-10-20
CHENGDU DIAO PHARMA GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no better method for separating and purifying the total saponins of Burnet burnet in production

Method used

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  • Sanguisorba saponins and preparation method of sanguisorbin I
  • Sanguisorba saponins and preparation method of sanguisorbin I

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the preparation of total saponins of Burnet burnet

[0031] Burnet decoction pieces 30kg, add 240L of 30% ethanol and extract under reflux for 1h, filter the residue with 180L of 30% ethanol and extract under reflux for 1h, combine the filtrate, add 600L of water to dilute, add sodium hydroxide solution (3.5kg of sodium hydroxide dissolved in 24L water), adjust the pH value to 14.2, let stand, centrifuge to obtain the filtrate, pass through the D101 type macroporous adsorption resin, the flow rate is 1.5 column volume / h, and use 4 column volumes of 30% ethanol to elute after finishing the drug solution, and then Then eluted with water until the effluent was colorless, and finally eluted with 70% ethanol of 3 times the volume of the column bed, collected the 70% ethanol effluent, concentrated in vacuo until a certain amount of substance was precipitated, then stopped concentrating, stood for more than 2 hours, and reduced Suction pressure filtration, wash w...

Embodiment 2

[0032] Embodiment 2: the preparation of total saponins of Burnet burnet

[0033] Burnet decoction pieces 30kg, add 300L of 60% ethanol and heat under reflux to extract for 1h, filter to obtain the filtrate, add 450L of water to dilute, add potassium hydroxide solution (2.5kg of sodium hydroxide dissolved in 25L of water) under stirring to adjust the pH value to 10.5, static place, centrifuge to obtain the filtrate, pass through the macroporous adsorption resin HPD100, the flow rate is 1.2 column volume / h, and then wash with water until the effluent is colorless, then use 4 times the volume of the column bed for elution, and collect the acetone The eluent was concentrated in vacuo, dried in a vacuum oven, and pulverized to obtain 1.09 kg of total glycosides of Burnet glucosides powder, with a yield of 3.63%. The content of total saponins of Burnet eucalyptus was 71% as detected by colorimetry, and it was detected by high performance liquid chromatography. The content of elm sap...

Embodiment 3

[0034] Embodiment 3: the preparation of total saponins of Burnet burnet

[0035] Burnet decoction pieces 30kg, add 240L of 45% ethanol and extract under reflux for 1h, then filter the residue with 180L of 30% ethanol and extract under reflux for 1h, combine the filtrate, add 600L of water to dilute, add calcium hydroxide suspension (3kg of calcium hydroxide suspended in 30L water) adjust the pH value to 14, let it stand, filter to obtain the filtrate, pass through the macroporous adsorption resin D101, the flow rate is 1.8 column volume / h, and then use 4 column volumes of 1.0% sodium hydroxide solution to elute , then eluted with water until the effluent was colorless, then eluted with 30% ethanol of 3 times the volume of the column bed, and finally eluted with 75% ethanol of 3 times the volume of the column bed, collected the 75% ethanol eluate, and concentrated in vacuo , until there is no alcohol smell, the remaining concentrate is dried in a vacuum oven and pulverized to o...

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Abstract

The invention relates to a method for separating and purifying sanguisorba saponins and sanguisorbin I by using alkaline liquid medicine through macroporous resin, comprising the following steps of: carrying out hot reflux extraction or percolation on cut-off sanguisorba, which is a traditional Chinese medicine and used as a raw material, more than one time with water or alcohol / water, and processing extracts by using alkali to regulate pH value to 10-15; adsorbing and separating the filtered extracts by using the macroporous resins, eluting the extracts by using alkaline water or water and about 30% of alcohol to remove impurities, and then desorbing and eluting by using organic solvents; carrying out decompression concentration, concentration, standing and filtration on eluent, and thencollecting sediments to obtain the sanguisorba saponins; or directly sedimentating by using the alkali, regulating the pH of the filtered liquid medicine to 4-6 after filtration, concentrating to recover alcohol; standing to separate out and obtain a sanguisorba saponins mixture, and washing the eluted resins by using the organic solvents for repeated use; and recrystallizing the sanguisorba saponins mixture by using alcohol water to obtain the sanguisorbin I with the content more than 98%. Saponins and other impurities are separated and extracted in the method, and the method has the advantages of low cost, high efficiency, simplicity, convenience and suitability for industrial production and can be used for obtaining high-purity sanguisorba saponins and sanguisorbin I.

Description

technical field [0001] The invention relates to a method for separating and purifying total saponins of Burnet eucalyptus and Saponin I of Burnet eucalyptus, belonging to the technical field of medicine. Background technique [0002] Burnet is a plant in the family Rosaceae. It contains active ingredients such as tannins, triterpenes, and saponins. It has multiple functions such as clearing heat and detoxifying, antibacterial, and antioxidative. It is a raw material for medicine and cosmetics. At present, there are many extraction methods for Burnet saponins, such as: after degreasing Burnet powder with petroleum ether, extract it with absolute ethanol; collect the extract, and recover it under reduced pressure; dissolve the extract in water, extract the water layer with n-butanol, collect The n-butanol layer was recovered under reduced pressure to obtain the crude Burnet saponin; the crude product was dissolved in water, saturated with sodium chloride, then extracted with n...

Claims

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Application Information

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IPC IPC(8): A61K36/739C07J63/00A61P39/06A61P31/04A61K125/00
Inventor 姬建新刘智勇李启发陈洪超黎秀丽白红艳李伯刚
Owner CHENGDU DIAO PHARMA GROUP
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