Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors

An elicitor and saponin technology, applied in the field of saponin accumulation, can solve the problems of low yield, ecological environment damage, long growth cycle, etc., and achieve the effect of high saponin content

Inactive Publication Date: 2015-09-02
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Ginsenosides, as a main active ingredient in ginseng, have low yields and have not yet been artificially synthesized
At present, ginseng mainly relies on field cultivation. However, the cultivated ginseng has complex cultivation techniques, long growth cycle, "old ginseng land" and ecological environment damage. People's needs, people try to improve the content of ginseng

Method used

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  • Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors
  • Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors
  • Method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0018] Example 1

[0019] Preparation of hydrolyzed milk protein aqueous solution mother solution: the hydrolyzed milk protein was dissolved in distilled water to prepare a solution with a concentration of 1 mg / ml, and filtered and sterilized through a 0.22 μm filter membrane.

[0020] Preparation of methyl jasmonate ethanol solution mother solution: methyl jasmonate was prepared into a solution with a concentration of 1 mg / ml in absolute ethanol, and sterilized by filtration through a 0.22 μm filter membrane.

Example Embodiment

[0021] Example 2

[0022] Adopt two kinds of elicitors to promote the method for saponin accumulation in adventitious root of ginseng, comprising the following steps:

[0023] Select well-grown ginseng adventitious roots and inoculate them into 2.5L of transformed 3 / 4MS liquid medium, in aseptic, 23±2°C, adjusted ventilation to 0.3vvm, cultivated for 29 days under natural light conditions, and added as elicitors. The hydrolyzed milk protein aqueous solution mother liquor and the methyl jasmonate ethanol solution mother liquor prepared in Example 1, make the hydrolyzed milk protein final concentration be 98mg / L, make the methyl jasmonate final concentration be 8mg / L, then use aseptic, 23 ± 2 ℃ , adjust the ventilation to 0.3vvm, cultivate for 2 days under natural light conditions, harvest and dry.

[0024] Preparation of the modified 3 / 4MS liquid medium: add sucrose to the 3 / 4MS liquid medium to make the final concentration 35g / L, add indolebutyric acid to make the final conce...

Example Embodiment

[0039] Example 3

[0040] Adopt two kinds of elicitors to promote the method for saponin accumulation in adventitious root of ginseng, comprising the following steps:

[0041] Select well-grown ginseng adventitious roots and inoculate them into 2.5L of transformed 3 / 4MS liquid medium, cultured for 30 days under natural light conditions at sterile, 23±2°C, adjusted ventilation to 0.4vvm, and added as an inducer. The hydrolyzed milk protein aqueous solution mother liquor and the methyl jasmonate ethanol solution mother liquor prepared in Example 1, make the final concentration of hydrolyzed milk protein be 99mg / L, make the final concentration of methyl jasmonate be 9mg / L, and then use aseptic, 23 ± 2 ℃. , adjust the ventilation to 0.4vvm, cultivate for 5 days under natural light conditions, harvest and dry.

[0042] Preparation of the modified 3 / 4MS liquid medium: add sucrose to the 3 / 4MS liquid medium to make the final concentration 30g / L, add indolebutyric acid to make the fi...

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Abstract

The invention discloses a method for accelerating accumulation of saponin in ginseng adventitious roots by adopting two types of inductors. The method comprises the following steps: picking the ginseng adventitious roots which grow well and inoculating into a modified 3/4 MS liquid culture medium; culturing for 28-30 days; adding a lactoalbumin hydrolysate water solution mother solution and a jasmonic acid methyl ester ethanol mother solution which are used as the inductors, wherein the final concentration of lactoalbumin hydrolysate is 98mg/L-100mg/L and the final concentration of jasmonic acid methyl ester is 8mg/L-10mg/L; and continuing to culture for 2-7 days, harvesting and drying. An experiment shows that the two inductors including lactoalbumin hydrolysate and jasmonic acid methyl ester can express the synergistic effect and do not have influences on secondary metabolism of the ginseng adventitious roots; the content of the saponin is high and evidences are provided for the large-scale production of ginsengs; and the industrial production of the ginseng saponin is realized and the day-by-day increasing requirements of people are met.

Description

technical field [0001] The invention belongs to the field of crop biotechnology, and in particular relates to a method for promoting saponin accumulation in adventitious roots of ginseng by using two elicitors. Background technique [0002] Ginseng is a perennial dicotyledonous plant of the family Araliaceae. It has the functions of enhancing immunity, preventing and treating cardiovascular diseases, protecting liver and liver, regulating blood sugar, anti-tumor, delaying aging, and enhancing learning and memory abilities. Commonly used in pharmaceuticals, health care medicines and cosmetics industries. [0003] Ginsenosides, as a class of main active ingredients in ginseng, have low yields and have not yet been artificially synthesized. At present, ginseng mainly relies on field cultivation. However, the cultivated ginseng has complex cultivation techniques, long growth cycle, "old ginseng land" and ecological environment damage. People's needs, people try to improve the ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高文远王娟李金鑫李静李建丽刘书杰
Owner TIANJIN UNIV
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