Method for taking two inducers to promote ginseng adventitious saponin accumulation

Abstract

The present invention discloses a method for taking two inducers to promote ginseng adventitious saponin accumulation, comprising the following steps: selecting well-grown ginseng adventitious and inoculating well-grown ginseng adventitious into 3 / 4MS liquid medium to culture 28 to 30 days, adding the hydrolysates milk protein solution mother liquid and the methyl jasmonate ethanol solution mother liquid as inducers to make the final concentration of hydrolysates milk protein solution become 98 ~ 100mg / L and the final concentration of methyl jasmonate ethanol solution become 8-10mg / L to culture for 2-7 days, harvesting and drying. Experiments show that the two inducers hydrolysates milk protein and the methyl jasmonate ethanol can exert a synergistic effect on the metabolism of ginseng adventitious secondary impact, have high saponin content, provide the basis for large-scale production of ginseng, achieve industrial production of ginseng saponin and meet people growing demand.

Description

technical field [0001] The invention belongs to the field of crop biotechnology, in particular to a method for promoting saponin accumulation in adventitious roots of American ginseng by using two elicitors. Background technique [0002] American ginseng is a perennial herb of the Araliaceae genus Panax, which was successfully introduced in my country in the 1970s. It is sweet, slightly bitter, cool in nature, enters the heart, lung, and kidney channels, and has various medical effects such as nourishing, strengthening the heart, hematopoiesis, invigorating the stomach, calming, lowering blood fat, anti-cancer, and anti-aging. Promising luxury and strength tonic. [0003] American ginseng saponins, as a main active ingredient in American ginseng, have low yields and have not yet been artificially synthesized. At present, American ginseng mainly relies on field cultivation. However, American ginseng has complex cultivation techniques, long growth cycle, "old ginseng land" a...

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Problems solved by technology

[0003] American ginseng saponins, as a main active ingredient in American ginseng, have low yields and have not yet been artificially synthesized

At present, American ginseng mainly relies on field cultivation. However, American ginseng has complex cultivation techniques, long growth cycle, "old ginseng land" and ecological environment damage. People's needs, people try to increase the content of secondary metabolites of American ginseng through various ways, such as tissue culture and reactor expansion culture, fed culture, two-step culture, precursor feeding, induction of hairy roots, elicitors, etc. addition of

[0004] So far, the synergistic effect of two elicitors using hydrolyzed milk protein and methyl jasmonate has not been reported in the adventitious root culture of American ginseng

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