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Ginseng cell culture medium and application thereof

A culture medium and basal medium technology, applied in the field of plant tissue culture, can solve the problems of poor genetic stability of cells and low saponin content, and achieve the effects of stable culture quality, high yield, and ecological environment protection

Inactive Publication Date: 2018-09-14
刘汉石
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a ginsenoside cell line and its establishment method for the problems of low saponin content and poor cytogenetic stability in the artificially induced ginseng cell line in the prior art

Method used

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  • Ginseng cell culture medium and application thereof
  • Ginseng cell culture medium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of ginseng callus induction medium

[0035] Prepare MS basal medium (see attached table 1 for the formula), add 0.5mg / LGA (gibberellin), 30g / L sucrose, and 0.6g / L agar to the MS basal medium. After the prepared medium is sterilized at high temperature, it is cooled and poured onto a plate for later use.

Embodiment 2

[0036] Embodiment 2 prepares ginseng cell culture medium

[0037] Prepare MS basal medium (see attached table 1 for formula), add 0.5mg / L 6-BA (6-benzylpurine), 4mg / L NAA (naphthalene acetic acid), 0.8g / L agar and 30g / L sucrose. After the prepared medium is sterilized at high temperature, it is cooled and poured onto a plate for later use.

Embodiment 3

[0038] The induction of embodiment 3 ginseng cells

[0039] (1) Preparation of ginseng callus: take wild ginseng roots as explants, remove the surface epidermis after disinfection, inoculate on the callus induction medium configured in Example 1, and cultivate to obtain ginseng callus,

[0040] (2) Cultivation of ginseng cells: get the ginseng callus in step (1), inoculate in the ginseng cell culture medium configured in Example 2, transfer once every 35 days,

[0041] (3) Subculture and screening of ginseng cells: the cells in step (2) are continuously subcultured, and three kinds of ginseng cell tissue lines are obtained by screening: white series, green series, and yellow series, through which the growth rate, ginsenoside The detection of the content determined that the yellow line was the cell line with better growth and the highest ginsenoside content.

[0042] The screening conditions for the yellow line in step (3) are: select cultures characterized by vigorous growth,...

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Abstract

The invention provides a ginseng cell induction medium and a ginseng cell induction method. The ginseng cell induction medium comprises an MS basic culture medium, 0.2-0.5mg / L of 6-BA (6-benzyladenine), 2-4mg / L of NAA (naphthylacetic acid) and 20-30g / L of sucrose. Ginseng cells prepared by the method are high in ginsenoside content and stable in heredity, influence of environment factors on the ginseng cells is low, the ginseng cells can be used as the ideal model of ginsenoside biological synthesis molecular regulation researches, an effective approach for achieving large-scale industrial production of the ginsenoside using the ginseng cells can be explored, and the method is worthy of popularization and application.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a ginseng adventitious root induction medium and application thereof. Background technique [0002] Medicinal plant ginseng is a traditional precious Chinese medicinal material in my country, and is known as the king of medicines. Its products have a broad market. Ginseng Panax ginseng C.A.Meyer is a plant of the genus Panax of Araliaceae, which is mainly distributed in Northeast China, Korea and Japan. Its secondary metabolite ginsenoside (ginsenoside) is a tetracyclic triterpene dammarane compound composed of isoprene units (five carbons), composed of panaxadiol and panaxatriol saponins, mainly distributed in the roots, followed by for stems and leaves. So far, there are more than 50 kinds of saponins whose structures have been isolated and determined. Modern studies believe that the main medicinal and active ingredients of ginseng are ginsenosides, which not only have p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04
CPCC12N5/0025C12N5/04
Inventor 刘汉石刘禹
Owner 刘汉石
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