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Earthworm microalgae small molecule peptide, as well as preparation method and application thereof

A technology of small molecular peptides and microalgae, applied in the field of agricultural fertilizers, can solve the problems of poor active ingredients and single functions, and achieve the effects of convenient operation, simple preparation method, and improved fruit quality

Inactive Publication Date: 2018-11-09
广西宾德利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although traditional seaweed fertilizers are rich in nutrients, their active ingredients are poor, their functions are single, and their application fields still have certain limitations.

Method used

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  • Earthworm microalgae small molecule peptide, as well as preparation method and application thereof
  • Earthworm microalgae small molecule peptide, as well as preparation method and application thereof
  • Earthworm microalgae small molecule peptide, as well as preparation method and application thereof

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preparation example Construction

[0023] In addition, the applicant specially designed a preparation method for the microalgae small molecule peptide of the present invention, including the following steps:

[0024] S101: weighing each raw material component in proportion, and then shearing and stirring the chlorella and / or the spirulina to obtain the chlorella pulp and / or the spirulina pulp.

[0025] S102: Heat the chlorella pulp and / or spirulina pulp obtained in S101 to 35-50°C, add fresh pineapple peel juice, stir for 10-20 minutes, and then keep warm at 40-50°C for 6-15 days.

[0026] S103: Add live earthworms to the product obtained in S102, cut for 5-10 minutes, then add papain and continue the enzymolysis at 40-50°C for 30-50 days at a constant temperature.

[0027] S104: Sampling test the content of amino acids, small molecular peptides, polypeptide gibberellins, gibberellin and gibberellic acid in the S103 product, stop enzymatic hydrolysis after reaching the standard, and then kill the enzyme at 60-8...

Embodiment 1

[0030] This embodiment provides a microalgae small molecular peptide. The raw material components include: 50 parts by weight of chlorella, 5 parts by weight of fresh pineapple peel juice, 2 parts by weight of papain and 20 parts by weight of live earthworms.

[0031] According to the above raw material components, adopt the method provided by the present invention to prepare microalgae small molecular peptides:

[0032] S101: weighing each raw material component in proportion, and then shearing and stirring the chlorella to obtain the chlorella pulp.

[0033] S102: heating the chlorella pulp obtained in S101 to 50° C., adding fresh pineapple peel juice, stirring for 20 minutes, and then keeping the temperature at 50° C. for 6 days.

[0034] S103: Add live earthworms to the product obtained in S102, cut for 5 minutes, then add papain and continue the enzymatic hydrolysis at 40°C for 50 days at a constant temperature.

[0035] S104: Sampling and testing the content of amino ac...

Embodiment 2

[0037] This embodiment provides a microalgae small molecular peptide. The raw material components include 20 parts by weight of spirulina, 10 parts by weight of fresh pineapple peel juice, 0.2 parts by weight of papain and 70 parts by weight of live earthworms.

[0038] According to the above raw material components, adopt the method provided by the present invention to prepare microalgae small molecular peptides:

[0039] S101: weighing each raw material component in proportion, and then shearing and stirring the spirulina to obtain a spirulina pulp.

[0040] S102: The temperature of the spirulina slurry obtained in S101 is raised to 35° C., fresh pineapple peel juice is added, stirred for 10 minutes, and then kept at 40° C. for 15 days.

[0041] S103: Add live earthworms to the product obtained in S102, cut for 10 minutes, then add papain and continue the enzymatic hydrolysis at 50°C for 30 days at a constant temperature.

[0042] S104: Sampling and testing the content of a...

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Abstract

The invention relates to an earthworm microalgae small molecule peptide, as well as a preparation method and application thereof. The earthworm microalgae small molecule peptide is prepared from the raw material components in parts by weight: 10 to 50 parts of chlorella and / or spirulina, 5 to 10 parts of fresh pineapple peel juice, 0.2 to 2 parts of papain, and 20 to 70 parts of living earthworms.The preparation method comprises the steps of shearing and stirring microalgae to obtain a microalgae pulp; after heating, adding the fresh pineapple peel juice, uniformly stirring, and preserving heat; adding the earthworms, shearing, adding the protease, continuously preserving heat, and carrying out enzymolysis; after the content of a bioactive substance reaches the standard, stopping enzymolysis, and then carrying out enzyme deactivation at high temperature so as to obtain the microalgae small molecule peptide. According to the earthworm microalgae small molecule peptide provided by the invention, the crop yield increment, the income increment, the disease resistance and the stress resistance can be effectively promoted, the plant immunity is improved, the fruit quality is improved, the culturing cost is greatly reduced, meanwhile, energy conservation and emission reduction are truly realized, the aim of green agriculture is achieved, and the sustainable agricultural development direction is guided.

Description

technical field [0001] The invention relates to the technical field of agricultural fertilizers, in particular to a small molecule peptide of earthworm microalgae and its preparation method and application. Background technique [0002] In recent years, in order to meet the demand for crops and pursue high yields of agricultural products, the long-term and extensive use of chemical fertilizers, pesticides, hormones and other chemical products in the process of planting agricultural products is a commonly used method in agricultural production. However, due to chemical fertilizers, pesticides, hormones and other chemical products remaining in crops, there is a great potential harm to human health. At present, the foliar fertilizers produced by most manufacturers are hormone-added fertilizers. Although this type of fertilizer has the effect of increasing agricultural production, long-term use will cause soil hardening, salinization, and hardening, including the decline in crop...

Claims

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Application Information

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IPC IPC(8): A01N65/40A01N63/02A01N63/00A01N37/46A01P21/00A01C21/00C05F17/00C05G3/00
CPCA01C21/00A01N37/46A01N63/00A01N63/10A01N65/00A01N65/40C05F1/00C05F17/00C05G3/00C05F11/00C05F17/20C05F11/08Y02W30/40
Inventor 白学宾
Owner 广西宾德利生物科技有限公司
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