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Protein electrophoretic separation and purification method and protein electrophoretic separation and purification device

An electrophoretic separation and purification device technology, which is applied in the field of protein separation, can solve problems such as the inability to separate protein mixtures in batches, and achieve the effects of facilitating batch production, improving separation purity, and concentrating the output liquid

Pending Publication Date: 2018-11-13
HUALAN BIOLOGICAL ENG CHONGQING +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a protein electrophoresis separation and purification method to solve the problem in the prior art that protein mixtures cannot be separated in batches

Method used

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  • Protein electrophoretic separation and purification method and protein electrophoretic separation and purification device
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  • Protein electrophoretic separation and purification method and protein electrophoretic separation and purification device

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Effect test

Embodiment 1

[0025] The components to be separated are: IgG

[0026] A protein electrophoresis separation and purification method, using an electrophoresis separation and purification device to separate the liquid to be separated according to the following steps:

[0027] (1) Adjust the pH of the fused plasma to 7.3, the ionic strength to 0.05-0.10, and control the temperature at 0-6°C;

[0028] (2) Control the electrophoresis power supply voltage in the electrophoresis separation and purification device between 50-300v, and the current is 25-30mA, and the linear flow velocity of the liquid to be separated into the sample feeding slit of the electrophoresis separation and purification device is 0.7-1cm / min;

[0029] (3) Due to the different charges on the surface of different proteins and the different resistance of the molecular size, different proteins will be separated in different bands during the downward movement of the feed liquid, and the IgG will flow out in a straight line in a...

Embodiment 2

[0032] The components to be separated are: IX factor

[0033] A protein electrophoresis separation and purification method, using an electrophoresis separation and purification device to separate the liquid to be separated according to the following steps:

[0034] (1) Adjust the pH of the fused plasma to 4.35, the ionic strength to 0.05-0.10, and control the temperature at 0-6°C;

[0035] (2) Control the electrophoresis power supply voltage in the electrophoresis separation and purification device between 50-300V, and the current is 25-30mA, and the linear flow velocity of the liquid to be separated into the sample feeding slit of the electrophoresis separation and purification device is 0.7-1cm / min;

[0036] (3) In an uncharged state, factor IX is separated at the fastest speed in a straight line in the electrophoretic separation and purification device, while other positively / negatively charged components are separated on both sides of the electrophoretic separation and p...

Embodiment 3

[0039] The components to be separated are: coagulation factor VIII

[0040] A protein electrophoresis separation and purification method, using an electrophoresis separation and purification device to separate the liquid to be separated according to the following steps:

[0041] (1) Adjust the pH of the fused plasma to 7.2, the ionic strength to 0.05-0.10, and control the temperature at 0-6°C;

[0042] (2) Control the electrophoresis power supply voltage in the electrophoresis separation and purification device between 50-300V, and the current is 25-30mA, and the linear flow velocity of the liquid to be separated into the sample feeding slit of the electrophoresis separation and purification device is 0.7-1cm / min;

[0043] (3) Blood coagulation factor VIII is separated at the fastest speed with a straight line trajectory in the electrophoretic separation and purification device in an uncharged state, while other positively / negatively charged components are separated on the t...

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Abstract

The invention discloses a protein electrophoretic separation and purification method. To-be-separated liquid can be separated by the aid of an electrophoretic separation and purification device. The protein electrophoretic separation and purification method includes steps that the PH (potential of hydrogen) value of the to-be-separated liquid is adjusted so that to-be-separated components are electrically neutral under the effects of isoelectric points of the to-be-separated components, the ionic strength of the to-be-separated liquid is adjusted to be 0.05-0.1, and the temperature of the to-be-separated liquid is 0-6 DEG C under the control; voltages of electrophoretic power sources in the electrophoretic separation and purification device range from 50 V to 300 V under the control, currents of the electrophoretic power sources are 25-30 mA, and the linear flow velocity of the to-be-separated liquid which flows into a sample loading liquid inlet slit of the electrophoretic separationand purification device is 0.7-1 cm / min; the to-be-separated liquid downwardly moves in the electrophoretic separation and purification device, and separated different protein components can be collected by isokinetic collecting grooves in the lower portion of the electrophoretic separation and purification device. Compared with the prior art, the protein electrophoretic separation and purification method and the electrophoretic separation and purification device have the advantages that batches of proteins can be separated by the aid of the protein electrophoretic separation and purificationmethod and the electrophoretic separation and purification device, and excellent separation effects can be realized by the protein electrophoretic separation and purification method and the electrophoretic separation and purification device.

Description

technical field [0001] The invention relates to the technical field of protein separation, in particular to a protein electrophoresis separation and purification method and a device thereof. Background technique [0002] The current separation and purification methods for biological macromolecular proteins and peptides are mostly precipitation, ion exchange, affinity chromatography and other technologies. Due to the method, the separation and purification accuracy is not high enough. If you want to obtain high-purity pharmaceutical-grade target , usually requires multiple steps to achieve. Multi-step separation and purification often lead to different degrees of damage to active molecules and the loss of target substances step by step, resulting in low recovery rates and reduced benefits. [0003] The electrophoresis technology of biomolecules uses the surface groups of biomolecules in the solution environment to have different properties and different amounts of charges du...

Claims

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Application Information

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IPC IPC(8): C07K1/26
CPCC07K1/26
Inventor 滕世超张宝献何英福王猛郭心怡李长明张建璀刘余江孟丽丽张学成
Owner HUALAN BIOLOGICAL ENG CHONGQING
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