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Preparation and analysis method of soluble ink melanin of marine animal

A technology of ink melanin and analysis method, which is applied in the field of preparation and analysis of soluble ink melanin of marine animals, can solve the problems of inability to comprehensively utilize ink, difficulty in separation and purification, and limited application, so as to shorten preparation time, increase drying yield, and maintain Effects of Activity and Stability

Inactive Publication Date: 2018-11-13
ALPHA FUJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many cephalopod production and processing enterprises such as squid and octopus. Their production produces a large amount of ink that cannot be comprehensively utilized, resulting in a lot of waste. Making full use of cephalopod ink is a technical problem that cephalopod production and processing enterprises need to solve urgently
Ink melanin has biological activities such as enhancing immune function, anti-tumor, anti-oxidation, anti-aging and antibacterial, but also has the disadvantages of being insoluble in water and difficult to separate and purify, which greatly limits its application in medicine, food and other fields

Method used

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  • Preparation and analysis method of soluble ink melanin of marine animal
  • Preparation and analysis method of soluble ink melanin of marine animal

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: selection of protease

[0024] Select pepsin, papain, neutral protease, trypsin, alkaline protease five kinds of proteases, the mass ratio of the first precipitate and protease solution in the hydrolysis condition is 1: 50, and the activity of protease solution is 4200 U / g, The hydrolysis time is 6 hours. Under the most suitable temperature and pH conditions for each protease, the marine cephalopod ink is incubated and enzymolyzed, and the light absorption value (260nm) of the product melanin is used as an index to select the hydrolysis effect on the ink. The best protease. According to Beer's law, the absorbance value is proportional to the concentration of the absorbing substance. The higher the concentration of melanin, the corresponding greater the absorbance value. Therefore, this study uses absorbance as a relative index to measure the level of melanin content. The melanin of marine animals has an obvious absorption peak (206 and 557 nm) in the ul...

Embodiment 2

[0028] Embodiment 2: process condition optimization:

[0029] A single factor experiment was carried out on the four factors of enzymolysis temperature, pH, enzyme dosage and time to determine the relevant factors and the appropriate range of each factor. On the basis of extracting single factor experiments, the process conditions were optimized by orthogonal experiments. Taking enzymatic hydrolysis temperature, pH value, enzyme activity, and hydrolysis time as the investigation objects, three levels were selected for each factor, and experiments were arranged to optimize the process conditions.

[0030]

[0031] At a temperature of 50 ℃, an enzyme dosage of 4200 U / g, and an enzymatic hydrolysis time of 6 hours, different pH values ​​of 6.8, 7.4, 8.0, 8.6, and 9.2 were selected for experiments to explore the effect of pH on the enzymatic hydrolysis process. When the pH of the enzyme solution is between 6.8 and 8.0, the absorbance value of the reaction product has a slow ...

Embodiment 3

[0033] Embodiment 3: process condition verification:

[0034] The optimal process parameters obtained by the orthogonal experiment were verified and compared with the melanin produced under the same conditions without enzymes, the results were compared with the water extraction method without enzymes. The enzymatic extraction optimized by the orthogonal experiment The absorbance value of the melanin was significantly higher, and the absorbance increased from 0.3841 to 0.6265.

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Abstract

The invention relates to a preparation and analysis method of soluble ink melanin of marine animals. The preparation and analysis method of the soluble ink melanin of the marine animals includes the steps of extracting cephalopod ink, soaking the ink with an equal volume of purified water and centrifuging to obtain a first precipitate, adding the first precipitate to a protease solution for hydrolysis, heating and then centrifuging the solution to obtain a second precipitate after the hydrolysis, conducting vacuum drying of the second precipitate to obtain a soluble ink melanin finished product, and extracting the soluble ink melanin finished product to dissolve a NaOH (sodium hydroxide) solution and conduct water-bath heating to then detect absorbance. The preparation and analysis methodof the soluble ink melanin of the marine animals has the advantages that with the above-mentioned technical scheme of extracting the ink melanin from the marine animals by means of oriented thermal enzymatic hydrolysis at 90 DEG C and high-speed centrifugation, proteins and the ink melanin can be directionally separated, and purity of melanin products can be improved; enzymatic extraction conditions are mild, which is conductive to maintaining the stability of the morphological structure of natural ink melanin; with absorbance as a method for determining the concentration of the melanin, the method is high in accuracy, and simple and easy to operate.

Description

technical field [0001] The invention relates to a method for preparing and analyzing melanin, in particular to a method for preparing and analyzing soluble melanin of marine animals. Background technique [0002] The word melanin comes from Greece and was first proposed by the Swiss chemist Berzelius. It is a water-insoluble biomacromolecule polymer and the only known natural endogenous organism that can protect organisms from radiation damage. Polymers, which are widely present in animals and plants. Marine animal ink melanin is mainly distributed in the Mollusca (Mollusca) cephalopod (Cephalopoda), there are about 650 species in existence, such as octopus (octopus), squid (commonly known as cuttlefish), squid commonly known as squid (Squid), nautilus (nautilus) )Wait. From the near shore to the open sea, from the surface to the depth below 4,500 meters. Ink melanin is a synonym for the biomacromolecular polymer contained in the ink of marine cephalopods such as cuttle...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54G01N21/31
CPCC09B61/00C09B67/0096G01N21/31
Inventor 郑春源唐文波戴金玉魏斌
Owner ALPHA FUJIAN BIOTECH
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