Quantitative detection method and device for upconverting fluorescent test strips

Abstract

The invention discloses a quantitative detection method and a quantitative detection device for upconverting fluorescent test strips. The quantitative detection method comprises the following steps: under the excitation of 980nm near-infrared laser, capturing a fluorescent signal emitted by a test strip standard substance by using an image sensor, extracting the brightness values of pixels corresponding to regions, where a T band and a C band are positioned, from the image sensor, and calculating a ratio of the average brightness value of a T-band region to the average brightness value of a C-band region to obtain a linear relationship between the ratio and the correspondingconcentration of an item to be detected; take a plurality of test strips to be detected for detecting different items, extracting the brightness values of the pixels corresponding to the regions, where the T band and the C band are positioned, on each test strip to be detected, and calculating the ratio of the average brightness value of the T-band region to the average brightness value of the C-band region on each test strip to be detected, and acquiring the concentration of each item to be detected according to the linear relationship obtained in the step(1).Through the quantitative detection method and the quantitative detection device, not only simultaneous detection of multiple upconverting fluorescenttest strips can be achieved, but also quantitative detection can be achieved, and the detection accuracy is high.

Description

technical field [0001] The invention relates to the technical field of quantitative detection of up-conversion fluorescent test paper, in particular to a quantitative detection method and device of up-conversion fluorescent test paper. Background technique [0002] Up-conversion luminescence is a kind of fluorescence luminescence mechanism with anti-Stokes effect. It usually emits short-wavelength fluorescence through up-conversion under the irradiation of 980nm near-infrared long-wavelength light, and under the doping of different elements and different doping ratios Fluorescence of different wavelengths is emitted. Since the fluorescence emission of substances in nature conforms to the Stokes effect, different substances will emit a certain amount of fluorescence under excitation light, which results in the appearance of background fluorescence of substances. The use of up-conversion fluorescence avoids the fluorescence excitation of the material itself, and only the targ...

AI Technical Summary

Problems solved by technology

This method has the advantages of high sensitivity and mature technology, but it cannot realize the simultaneous detection of multiple up-conversion fluorescent test strips. For tumor marker detection and other occasions that require simultaneous detection of multiple indicators, the efficiency is far from meeting the requirements of multi-index detection. needs

Images