Tissue culture and regeneration system establishment of ground fruit

A technology of tissue culture and ground fruit, applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient, difficult to meet market requirements, slow growth, etc., achieve the effect of improving reproduction efficiency, solving browning phenomenon, and promoting plant rooting

Active Publication Date: 2021-05-18
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cuttings and layering are usually used in ground fruit production, and the reproduction coefficient is also low, and the growth is slow, which is difficult to meet market requirements.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of ground fruit tissue culture method, comprises the steps:

[0019] (1) Explant selection and disinfection process: select tender stems in good growth condition, disinfect them with alcohol for 30 seconds, and disinfect them with mercury liter for 12 minutes to obtain sterile terminal buds.

[0020] (2) terminal bud callus induction culture, the terminal bud in (1) is inoculated on the callus induction medium to cultivate; the terminal bud callus induction medium formula: add 6-benzyl in MS medium Aminoadenine (6-BA) 0.4mg·L -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0.

[0021] (3) The subculture proliferation culture of shoots, the terminal buds with callus in (2) are inoculated to the subculture medium, and the subculture medium formula: add 6-benzylaminoadenine ( 6-BA) 1.0mg·L -1 , naphthaleneacetic acid (NAA) 1.0mg·L -1 , phenylthiadiazole (TDZ) 0.5mg·L -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH valu...

Embodiment 2

[0025] A kind of ground fruit tissue culture method, comprises the steps:

[0026] (1) Explant selection and disinfection process: Select tender stems with good growth conditions, disinfect them with alcohol for 30 seconds, and disinfect them with mercury chloride for 12 minutes to obtain sterile stem segments with buds.

[0027] (2) primary culture, inoculate the budding stem section in (1) into the primary medium for cultivation, and the primary medium is to add 0.4mg L of 6-benzylaminoadenine (6-BA) in MS medium -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0;

[0028] (3) callus induction culture, the stem segment in (1) is inoculated in the callus induction medium and cultivated; the stem segment callus induction medium formula: add naphthaleneacetic acid (NAA) 0.5mg in MS medium · L -1 ;20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0.

[0029] (4) callus differentiation culture, the callus induced in (3) is inse...

Embodiment 3

[0034] A kind of ground fruit tissue culture method, comprises the steps:

[0035](1) Explant selection and disinfection process: Select tender stems with good growth conditions, disinfect them with alcohol for 30 seconds, and disinfect them with mercury chloride for 12 minutes to obtain sterile stem segments with buds.

[0036] (2) primary culture, inoculate the budding stem section in (1) into the primary medium for cultivation, and the primary medium is to add 0.4mg L of 6-benzylaminoadenine (6-BA) in MS medium -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0;

[0037] (3) Insert the tissue cultured shoots through primary culture in (2) into the rooting medium to induce rooting culture, and directly obtain the tissue cultured shoots; the rooting medium is to add indole butyric acid in 1 / 2MS medium (NAA)0.1mg·L -1 , carbon powder 1.0g·L -1 , 20-25g·L -1 Sucrose, and adjust the pH value to 5.8-6.0;

[0038] (4) After the rooting culture in (...

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PUM

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Abstract

The invention relates to a method for tissue culture and propagation of ground fruit. The method comprises the following steps: (1) disinfection of explants; (2) induction and proliferation culture of stem segments with buds; (3) callus differentiation culture; (4) rooting culture of tissue cultured seedlings (5) hardening and transplanting. The present invention improves the method for inducing and culturing different explants of Digo, solves the problems of low multiplication coefficient, low differentiation rate, long rooting cycle, etc. The multiplication coefficient, differentiation rate and rooting rate are improved, and the survival rate can reach 90%.

Description

Technical field: [0001] The invention belongs to the technical field of plant tissue culture, and relates to a culture method, in particular to a tissue culture method of a novel garden ornamental plant-Digo. Background technique: [0002] Ground fruit (Ficus tikoua Bur.), also known as ground loquat, wild sweet potato, ground pomegranate, etc. It is an evergreen creeping woody vine of the genus Moraceae. The ground fruit has a neat plant shape and is an excellent ground cover plant in the garden plant configuration, suitable for greening in parks or places that are easily trampled by tourists. The ground fruit is semi-shade-tolerant, barren-resistant, moderate in height, and has crisscross stems and vines. It is an excellent plant for windbreak and sand fixation. It can also be used as a three-dimensional greening material in urban garden landscape construction, and can also be used as a hanging bonsai for fruit viewing and leaf viewing. [0003] Cuttings and layering ar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张彦妮李芊夏
Owner NORTHEAST FORESTRY UNIVERSITY
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