Tissue culture and regeneration system establishment of ground fruit
A technology of tissue culture and ground fruit, applied in the field of plant tissue culture, can solve the problems of low reproduction coefficient, difficult to meet market requirements, slow growth, etc., achieve the effect of improving reproduction efficiency, solving browning phenomenon, and promoting plant rooting
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Embodiment 1
[0018] A kind of ground fruit tissue culture method, comprises the steps:
[0019] (1) Explant selection and disinfection process: select tender stems in good growth condition, disinfect them with alcohol for 30 seconds, and disinfect them with mercury liter for 12 minutes to obtain sterile terminal buds.
[0020] (2) terminal bud callus induction culture, the terminal bud in (1) is inoculated on the callus induction medium to cultivate; the terminal bud callus induction medium formula: add 6-benzyl in MS medium Aminoadenine (6-BA) 0.4mg·L -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0.
[0021] (3) The subculture proliferation culture of shoots, the terminal buds with callus in (2) are inoculated to the subculture medium, and the subculture medium formula: add 6-benzylaminoadenine ( 6-BA) 1.0mg·L -1 , naphthaleneacetic acid (NAA) 1.0mg·L -1 , phenylthiadiazole (TDZ) 0.5mg·L -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH valu...
Embodiment 2
[0025] A kind of ground fruit tissue culture method, comprises the steps:
[0026] (1) Explant selection and disinfection process: Select tender stems with good growth conditions, disinfect them with alcohol for 30 seconds, and disinfect them with mercury chloride for 12 minutes to obtain sterile stem segments with buds.
[0027] (2) primary culture, inoculate the budding stem section in (1) into the primary medium for cultivation, and the primary medium is to add 0.4mg L of 6-benzylaminoadenine (6-BA) in MS medium -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0;
[0028] (3) callus induction culture, the stem segment in (1) is inoculated in the callus induction medium and cultivated; the stem segment callus induction medium formula: add naphthaleneacetic acid (NAA) 0.5mg in MS medium · L -1 ;20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0.
[0029] (4) callus differentiation culture, the callus induced in (3) is inse...
Embodiment 3
[0034] A kind of ground fruit tissue culture method, comprises the steps:
[0035](1) Explant selection and disinfection process: Select tender stems with good growth conditions, disinfect them with alcohol for 30 seconds, and disinfect them with mercury chloride for 12 minutes to obtain sterile stem segments with buds.
[0036] (2) primary culture, inoculate the budding stem section in (1) into the primary medium for cultivation, and the primary medium is to add 0.4mg L of 6-benzylaminoadenine (6-BA) in MS medium -1 , 20-25g·L -1 Sucrose, 6-7g·L -1 Agar, and adjust the pH value to 5.8-6.0;
[0037] (3) Insert the tissue cultured shoots through primary culture in (2) into the rooting medium to induce rooting culture, and directly obtain the tissue cultured shoots; the rooting medium is to add indole butyric acid in 1 / 2MS medium (NAA)0.1mg·L -1 , carbon powder 1.0g·L -1 , 20-25g·L -1 Sucrose, and adjust the pH value to 5.8-6.0;
[0038] (4) After the rooting culture in (...
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