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Microwell plate cleaning device for immunoassay experiment and use method

A technology for immunoassay and cleaning device, which is applied in the direction of using liquid cleaning methods, cleaning methods and utensils, chemical instruments and methods, etc., can solve the problems of cross-contamination, affecting the experimental results, and leaving a lot of lotion, and achieves good use effect. , accurate position, good effect of injection

Pending Publication Date: 2018-11-20
ACCURACY TIANJIN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is: if the aspiration needle is too far from the bottom of the well, a lot of washing solution may remain, and if the aspiration needle is too close to the bottom of the well, it may touch the conjugate on the well wall
This will affect the experimental results. In addition, during the cleaning process, the aspiration needle will come into contact with the reaction liquid, which may also cause cross-contamination.
Another cleaning method is to use the centrifugal force generated by the rotation of the micro-orifice plate to remove the lotion. This device only solves the problem of cleaning the lotion, and the liquid thrown out by the centrifugal force will be scattered around, which is not conducive to cleaning; huge centrifugal force It also makes the problem of load balance prominent, and the effect of using it in specific applications is not ideal

Method used

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  • Microwell plate cleaning device for immunoassay experiment and use method
  • Microwell plate cleaning device for immunoassay experiment and use method
  • Microwell plate cleaning device for immunoassay experiment and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A microplate cleaning device used in immunoassay experiments, which consists of a frame 12, which is connected to a mixing motor 6 and a rocker motor 7 and fixed by screws, and the rocker motor 7 is connected to the rocker arm 2, the rocker arm 2 is connected to the rocker arm synchronization wheel 11, the mixing motor 6 is connected to the mixing tray 5 with an eccentric wheel, and the mixing tray 5 is connected to the magnet frame 4, so The top of the frame 12 is connected with a liquid injection head moving motor 9, and the liquid injection head moving motor 9 is connected with the liquid injection head 3.

Embodiment 2

[0022] A microplate cleaning device used in immunoassay experiments described in Example 1, wherein the frame 12 is connected to the waste liquid bin 8, the top of the frame 12 is connected to the protective cover 1, and the machine The top of the frame 12 is connected to the washing liquid tank 11, and the magnet frame 4 is connected to the magnet base.

Embodiment 3

[0024] A microplate cleaning device used in an immunoassay experiment described in embodiment 2, the magnet base includes a base 13, and the base 13 is connected to the left ear plate 14 and the right ear plate 15, and the base 13 is inserted into the cylindrical magnet of the magnet holder 4, the base 13 is connected to the microporous plate 16, the base 13 and the microporous plate 16 are opened with a set of communicating micro-holes 17, the The micro sockets 17 are parallel to each other laterally and parallel to each other vertically, and the sockets 17 correspond to the liquid injection head 3.

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Abstract

Provided are a microwell plate cleaning device for an immunoassay experiment and a use method. The microwell plate cleaning device is composed of a rack (12), and the rack (12) is connected with an even mixing motor (6) and a rocker arm motor (7) and fixed through a screw. The rocker arm motor (7) is connected with a rocker arm (2), and the rocker arm (2) is connected with a rocker arm synchronizing wheel (11). The even mixing motor (6) is connected with a even mixing tray (5) with an eccentric wheel, and the even mixing tray (5) is connected with a magnetic frame (4), and the top of the rack(12) is connected with a liquid injection head moving motor (9) which is connected with a liquid injection head (3). The microwell plate cleaning device is used for microwell plate cleaning in the immunoassay experiment.

Description

Technical field: [0001] The invention relates to a microwell plate cleaning device used in an immunoassay experiment and a use method. Background technique: [0002] Immunoassay is an analysis method that uses the specific binding reaction of antigen and antibody to detect various substances (drugs, hormones, proteins, microorganisms, etc.); the enzyme-linked immunoassay method, chemiluminescence immunoassay method and polymerase chain reaction are all When it comes to the form of a microplate as a reaction carrier, it is necessary to clean and remove unbound substances during the experiment. The traditional main cleaning method is to soak the liquid injection needle with lotion, and then use the liquid suction needle to remove the lotion. The disadvantage of this method is that if the suction needle is too far from the bottom of the hole, a lot of lotion may remain, and if the suction needle is too close to the bottom of the hole, it may touch the combination of the hole wall. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B08B3/08B08B3/10B03C1/02
CPCB03C1/02B08B3/08B08B3/10
Inventor 程晓宇
Owner ACCURACY TIANJIN BIOTECH CO LTD
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