Human Y chromosome SNP typing method using high-throughput DNA hybrid chip

Abstract

The invention discloses a human Y chromosome SNP typing method using a high-throughput DNA hybrid chip. The human Y chromosome SNP typing method comprises the following steps: performing site homologycomparison based on Y chromosome SNP detection need, and comparing verified sites in a database; producing according to a designed chip; extracting gDNA from saliva or an oral swab sample collected from a subject; according to an Axiom chip detection process, automatically preparing the chip; scanning the prepared gene chip by using a GeneTitan MC instrument; analyzing a gene chip scanning resultby using software, and calculating the genotype of each detected SNP site; based on the detected SNP typing result, calculating a Y-DNA haplogroup according to an ISOGG Y-DNA tree. Application of a hybrid SNP chip to Y chromosome has the advantages of low nucleic acid preparation cost, low detection cost, flexible SNP site customization and high detection rate; uncertainty of a result due to surname change, uxorilocal marriage and the like in original family and surname researches and the like is reduced; the amount of the gDNA required for experimental detection is relatively low, and the detection cost is lower.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a human Y chromosome SNP typing method using a high-throughput DNA hybridization chip. Background technique [0002] At present, there is still a lack of reliable methods and marker databases based on family information for the Chinese population in the study of molecular biological (gene) markers in human immediate male relatives (Wu Fu Nei family). The Y-DNA haplogroup is a common method for genetic markers of male members of the family, but due to historical issues such as surname change, adoption, and matrilineal, the Y-DNA haplogroup may not be single in each family, and the Y chromosome composition is relatively complex. It also brings difficulty to the selection of genetic markers in male family members. [0003] Existing technical solutions: [0004] Hyplotyping experiment and calculation technology summary of the existing Y chromosome (may include commercially...

AI Technical Summary

Problems solved by technology

[0009] The cost of testing the Y chromosome by next-generation sequencing is relatively high, and the requirements for the total amount and quality of individual DNA are high

[0011] Existing analysis models (such as ISOGG Y-DNA tree) are mainly aimed at the global population, and lack background data for Chinese family markers

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