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Total bile acid assay kit and assay method thereof

The technology of a total bile acid and kit is applied in the field of total bile acid determination kit and its measurement, which can solve the problems of unsatisfactory anti-interference ability, achieve enhanced anti-interference ability, enhanced enzyme activity stability, and good measurement accuracy Effect

Inactive Publication Date: 2018-11-23
广州市伊川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many types of stabilizers used in existing kits, although the stability can be improved, but the anti-interference ability is often not ideal

Method used

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  • Total bile acid assay kit and assay method thereof
  • Total bile acid assay kit and assay method thereof
  • Total bile acid assay kit and assay method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment one total bile acid assay kit (formula two)

[0026] The total bile acid assay kit includes reagent R1 and reagent R2, and the reagent R1 includes the following components and their concentrations: Tris 12g / L, 6N HCL 20g / L, thiooxidized coenzyme I 1g / L , KCL 10g / L, NaN 3 1g / L; the reagent R2 includes the following components and their concentrations: Tris 12g / L, 6N HCL 20g / L, reduced coenzyme I 5g / L, 3a-hydroxysteroid dehydrogenase 10KU / L, Polyethylene glycol 1200 100g / L, betaine solution 1ml / L, NaN 3 1g / L. The concentration of betaine solution is 2mol / L.

Embodiment 2

[0027] Embodiment two total bile acid assay kit

[0028] The total bile acid assay kit includes reagent R1 and reagent R2, and the reagent R1 includes the following components and their concentrations: Tris 15g / L, 6N HCL 20g / L, thiooxidized coenzyme I 1.2g / L L, KCL 8g / L, NaN 3 1g / L; the reagent R2 includes the following components and their concentrations: Tris 15g / L, 6N HCL 20g / L, reduced coenzyme I 6g / L, 3a-hydroxysteroid dehydrogenase 12KU / L, Polyethylene glycol 1200 80g / L, betaine solution 0.8ml / L, NaN 3 1g / L. The concentration of betaine solution is 2mol / L.

Embodiment 3

[0029] Embodiment three total bile acid assay kit

[0030] The total bile acid determination kit includes reagent R1 and reagent R2, and the reagent R1 includes the following components and their concentrations: Tris 10g / L, 6N HCL 18g / L, thiooxidized coenzyme I 1g / L , KCL 10g / L, NaN 3 1g / L; the reagent R2 includes the following components and their concentrations: Tris 10g / L, 6N HCL 18g / L, reduced coenzyme I 5g / L, 3a-hydroxysteroid dehydrogenase 10KU / L, Polyethylene glycol 1200 100g / L, betaine solution 0.8ml / L, NaN 3 1g / L. The concentration of betaine solution is 2mol / L.

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Abstract

The invention provides a total bile acid assay kit, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from the following components with the concentrations: 10-20g / L trishydroxymethylaminomethane, 16-25g / L 6N HCL, 0.6-2g / L thiooxidized coenzyme I and 6-15g / L KCL; the reagent R2 is prepared from the following components with the concentrations: 10-20g / L trishydroxymethylaminomethane, 16-25g / L 6N HCL, 4-8g / L reduced coenzyme I, 6-15KU / L 3 alpha-hydroxysteroid dehydrogenase, 80-130g / L polyethylene glycol and 0.4-1.1ml / L betaine solution. The invention belongs to the technical field of biological detection. According to the total bile acid assay kit provided by the invention, the anti-interference ability is significantly enhanced while the stability of the reagents is improved, and is good in accuracy when being used for determination of total bile acid.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a total bile acid assay kit and a assay method thereof. Background technique [0002] Bile acids (3α-hydroxysteroids) are an important component of bile, mainly including glycocholic acid, deoxycholic acid, chenodeoxycholic acid, and lithocholic acid. These bile acids are collectively called total bile acids ( TBA). The level of bile acids in serum is an important evidence to reflect liver parenchyma damage, especially in acute hepatitis, chronic active hepatitis, ethanol liver injury and liver cirrhosis, there are more sensitive changes. When liver cells are damaged, the content of bile acids in the blood increases. Existing studies believe that the determination of bile acids in serum has a sensitivity of more than 70% and a specificity of more than 90% for the diagnosis of hepatobiliary diseases. Changes in cholesterol and cholesteryl esters come earlier, so it...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 刘光华杨玉军陈楚华刘秋明
Owner 广州市伊川生物科技有限公司
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