Ultrasonic-assisted extraction method of flaxseed protein

A technology for ultrasonic-assisted extraction and flaxseed protein, which is applied in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems that hinder the industrialization of flaxseed protein extraction and the high cost of flaxseed, so as to reduce energy consumption and Cost, low cost, and the effect of reducing production difficulty

Inactive Publication Date: 2018-11-27
广州利众生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings and deficiencies of the prior art, the present invention provides a method for ultrasonically assisted extraction of flaxseed protein isolates, thereby obtaining high-purity proteins; at the same time, the present invention improves the obstacles hindered by the high cost of producing degreased and degummed flaxseeds. Issues related to the industrialization of flaxseed protein extraction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for ultrasonically assisted extraction of flaxseed protein, comprising the following steps:

[0022] 1) Press the linseed to extract oil to obtain linseed meal, fully crush the linseed meal, pass through 30 mesh, 60 mesh, and 100 mesh sieves in turn, and collect the linseed meal powder after passing through the 100 mesh sieve for later use;

[0023] 2) Preparation of protein isolate and determination of protein content: Mix 200 g of the linseed meal powder obtained in step 1) with distilled water at a mass ratio of 1:25, adjust the pH value to 9 with alkali, and soak under the condition of ultrasonic power of 340W. Extract for 40min, centrifuge at 3200r / min for 15min, collect the supernatant, and measure the protein content in the supernatant by Kjeldahl method; adjust the pH value of the supernatant to the isoelectric point of protein 4.4 with 0.1mol / mL hydrochloric acid, 3200r / min centrifuged for 30min to collect the precipitate;

[0024] 3) Using freeze-dr...

Embodiment 2

[0027] A method for ultrasonically assisted extraction of flaxseed protein, comprising the following steps:

[0028] 1) Press the linseed to extract oil to obtain linseed meal, fully crush the linseed meal, pass through 30 mesh, 60 mesh, and 100 mesh sieves in turn, and collect the linseed meal powder after passing through the 100 mesh sieve for later use;

[0029] 2) Preparation of protein isolate and determination of protein content: mix 200 g of the linseed meal powder obtained in step 1) with distilled water at a mass ratio of 1:35, adjust the pH value to 10 with alkali, soak under the condition of ultrasonic power of 380W Extract for 60min, centrifuge at 3500r / min for 20min, collect the supernatant, and measure the protein content in the supernatant by Kjeldahl method; adjust the pH value of the supernatant to the isoelectric point of protein 4.4 with 0.1mol / mL hydrochloric acid, 3500r Centrifuge for 40min at 1 / min to collect the precipitate;

[0030] 3) Using freeze-dry...

Embodiment 3

[0033] A method for ultrasonically assisted extraction of flaxseed protein, comprising the following steps:

[0034] 1) Press the linseed to extract oil to obtain linseed meal, fully crush the linseed meal, pass through 30 mesh, 60 mesh, and 100 mesh sieves in turn, and collect the linseed meal powder after passing through the 100 mesh sieve for later use;

[0035] 2) Preparation of protein isolate and determination of protein content: Mix 200 g of the linseed meal powder obtained in step 1) with distilled water at a mass ratio of 1:30, adjust the pH value to 9.5 with alkali, soak under the condition of ultrasonic power of 360W Extract for 50min, centrifuge at 3300r / min for 18min, collect the supernatant, and measure the protein content in the supernatant by Kjeldahl method; adjust the pH value of the supernatant to the isoelectric point of protein 4.4 with 0.1mol / mL hydrochloric acid, 3300r / min Centrifuge for 35min, collect the precipitate;

[0036] 3) Using freeze-drying t...

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Abstract

The invention belongs to the technical field of food processing and particularly relates to an ultrasonic-assisted extraction method of flaxseed protein. The invention also discloses an ultrasonic-assisted extraction method of flaxseed protein, comprising the steps of 1) fully crushing flaxseed meal, screening, and collecting flaxseed meal powder for use; 2) subjecting the flaxseed meal powder toultrasonic-assisted distillate extraction to obtain flaxseed protein isolate, centrifuging, collecting supernate, and measuring protein content in the supernate; using an acid to adjust pH of the supernate, centrifuging, and collecting precipitate; 3) lyophilizing, and precipitating to obtain flaxseed protein. The method herein helps effectively extract flaxseed protein under high extraction rate;the process steps are simple; operations are convenient; the cost is low. The prepared flaxseed protein has high purity and has a promising application prospect.

Description

technical field [0001] The invention belongs to the technical field of food processing, in particular to a method for ultrasonically assisted extraction of linseed protein. Background technique [0002] Flaxseed is one of the main oil crops in my country. The outermost layer of flaxseed is the seed coat, which contains a large amount of flaxseed gum, fiber and a small amount of protein, accounting for about 30%-40% of the weight of flaxseed; the main part of flaxseed oil production is the embryo and endosperm of the inner layer of flaxseed , contains a lot of protein and fat. The oil content of linseed is generally 40%-50%; it is rich in a variety of unsaturated fatty acids. According to literature reports, the content of unsaturated fatty acids in linseed oil accounts for about 88% of the total fatty acids, which contains 22.3% of linoleic acid; oleic acid 14%; alpha-linolenic acid 50%. In my country, flaxseed is mainly used for oil extraction, and the development of oth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14
CPCC07K1/145
Inventor 汪勇张震葛佳佳邓凤桂
Owner 广州利众生物科技有限公司
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