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A modification method of Radix Astragali polysaccharide and application of the modified Radix Radix Polysaccharide

A modification method and technology of heptostilbene polysaccharide are applied in the directions of medical preparations containing active ingredients, pharmaceutical formulas, allergic diseases, etc., which can solve the problems of difficulty in later purification, low solubility, and low extraction rate, and achieve good application and development. Prospects, the effect of improving biological activity, improving immunity

Active Publication Date: 2021-04-30
LANZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems existing in the existing traditional hot water extraction method: low extraction rate, poor solubility, and difficult purification in the later stage, and the obtained natural polysaccharides have large molecular weight and volume, high viscosity, low solubility, and bioavailability. Inferior defects

Method used

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  • A modification method of Radix Astragali polysaccharide and application of the modified Radix Radix Polysaccharide
  • A modification method of Radix Astragali polysaccharide and application of the modified Radix Radix Polysaccharide
  • A modification method of Radix Astragali polysaccharide and application of the modified Radix Radix Polysaccharide

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Experimental program
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Effect test

Embodiment 1

[0065] The modification method of Radix Astragali polysaccharide specifically comprises the following steps:

[0066] (1) Radix Astragali, washed, crushed through an 80-mesh sieve, degreased once with 3 times the amount of ethyl acetate and 85% ethanol, 0.5h each time, and dried.

[0067] (2) Weigh 1.0 g of defatted Radix Radix Radix Dry Powder, add 10 mL of tap water, add citric acid-sodium citrate buffer to adjust pH=5, and perform enzymolysis with a compound enzyme, the amount of which is 1.0% of the mass of Radix Radix Radix %, the enzymatic hydrolysis temperature is 45°C, the enzymatic hydrolysis time is 1h, and no inactivation is required after enzymatic hydrolysis; the compound enzyme is composed of cellulase and papain, and the mass ratio of the two is 2:1.

[0068] (3) After enzymatic hydrolysis with complex enzymes, ultrasonication is performed, ultrasonic temperature is 60°C, ultrasonic power is 40W, ultrasonic time is 30min, ultrasonic treatment is performed once, ...

Embodiment 2

[0069] Example 2 Preparation of Astragalus Polysaccharides by Traditional Hot Water Extraction and Combined Enzyme Combined with Ultrasonic Modification of Astragalus Polysaccharides

[0070] (1) Traditional hot water extraction method: Take 1.0g of defatted medicinal powder, add 10mL of tap water, warm soak at 60°C for 3 times, each time for 1h, filter, combine the filtrate, concentrate the filtrate to 1 / 3 of the volume of the filtrate, add ethanol to make The volume concentration of ethanol is 70%, standing overnight at 4° C., centrifuging to obtain a precipitate, and vacuum freeze-drying the precipitate to obtain hot-water-extracted Heqi polysaccharide (HPS-R). The polysaccharide yield was 6%, and the polysaccharide content was 80%.

[0071] (2) Preparation of Heqi polysaccharide (HPS-MC1) and Heqi polysaccharide (HPS-MC2) by compound enzyme combined with ultrasonic modification of Heqi polysaccharide:

[0072] The preparation method of step (1) and step (2) is identical w...

Embodiment 11

[0114] The modification method of Radix Astragali polysaccharide specifically comprises the following steps:

[0115] (1) Radix Astragali, washed, crushed through a 50-mesh sieve, degreased twice with 5 times the amount of ethyl acetate and 95% ethanol, 2 hours each time, and dried.

[0116] (2) Weigh 1.0 g of defatted Radix Radix Dry Powder, add 30 mL of tap water, add citric acid-sodium citrate buffer solution to adjust pH=3, and carry out enzymolysis with compound enzyme, the amount of compound enzyme added is 6.0% of the mass of Radix Radix Radix %, the enzymatic hydrolysis temperature is 40°C, the enzymatic hydrolysis time is 2 hours, and no inactivation is required after enzymatic hydrolysis; the compound enzyme is composed of cellulase and papain, and the mass ratio of the two is 5:1.

[0117] (3) After enzymatic hydrolysis with complex enzymes, ultrasonication is performed, ultrasonic temperature is 30°C, ultrasonic power is 60W, ultrasonic time is 50min, ultrasonic tr...

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Abstract

The invention provides a method for modifying Radix Astragalus polysaccharides. First, hydrolyze Radix Radix Radix Radix with compound enzyme; then, perform ultrasonic treatment; finally, precipitate with ethanol; the compound enzyme is composed of cellulase and papain; Ethanol precipitation is to add ethanol to make the volume concentration of ethanol reach 30-85%. The invention provides a method for modifying the polysaccharide of Radix Astragalus, through a molecular modification technology combining enzymatic hydrolysis and ultrasound, the yield, purity and water solubility of polysaccharides of Radix Astragali can be effectively improved; Degradation, grafting and cross-linking reactions, thereby reducing the molecular weight of polysaccharides, changing the spatial conformation, and improving the biological activity of polysaccharides, is a modification technology that is simple, convenient, economical, environmentally friendly, high in efficiency, low in energy consumption, and pollution-free. The astragalus polysaccharide modified by the method of the present invention has biological activities such as anti-liver damage, anti-hepatic fibrosis, anti-osteoporosis, anti-gastric ulcer, hypoglycemic, anti-aging, anti-oxidation, and improving immunity.

Description

technical field [0001] The invention relates to a simple, convenient, economical and environment-friendly molecular modification technology, that is, a method for modifying astragali polysaccharides with compound enzymes combined with ultrasound, and also relates to the application of the modified hemorrhoids polysaccharides. Background technique [0002] Nowadays, with the rapid development of sugar technology and sugar science, polysaccharides with low toxicity, no residue, no drug resistance and significant activity have become research hotspots. It has been confirmed that polysaccharides of traditional Chinese medicine have various biological activities such as promoting immune regulation, anti-tumor, anti-virus, anti-oxidation, anti-inflammation, anti-stress, anti-radiation and anti-aging. Studies have found that the biological activity of polysaccharides is closely related to the spatial structure, molecular weight, monosaccharide composition, extraction method and mol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P1/16A61P19/10A61P1/04A61P3/10A61P39/06A61P37/02
CPCA61K31/715A61P1/04A61P1/16A61P3/10A61P19/10A61P37/02A61P39/06C08B37/00
Inventor 赵良功封士兰杨秀艳
Owner LANZHOU UNIVERSITY
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